Recombinant Human His6-UBE2J1 Protein, CF

Catalog # Availability Size / Price Qty
E2-750-100
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Recombinant Human His6-UBE2J1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-UBE2J1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2J1 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2J1 protein
Contains an N-terminal 6-His tag and the C-terminal transmembrane and lumenal amino acid sequences have been removed
Accession #
Predicted Molecular Mass
32 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-750

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2J1

Ubiquitin-conjugating Enzyme E2, J1 (UBE2J1), also known as HsUbc6e, is a 318 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of approximately 35 kDa (1). The human protein shares 92% and 85% aa sequence identity with the mouse and rat orthologs, respectively. UBE2J1 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (2). It localizes to endoplasmic reticulum (ER) membranes where it participates in ER-associated degradation (ERAD) of misfolded proteins (1-3). In response to ER stress, UBE2J1 is phosphorylated on Ser184 in a PERK-dependent manner (2). It has also been shown to function with the HRD1 Ubiquitin ligase (E3) to promote the ubiquitination and degradation of misfolded MHC class I heavy chain via ERAD (3). Additionally, UBE2J1 may have a preventative role in early-onset systemic amyloidosis. It has a redundant role, along with the UBE2G2 E2 enzyme, in promoting the degradation of a mutant form of Transthyretin that is associated with this disease (4). This protein contains an N-terminal 6-His tag.

References
  1. Lenk, U. et al. (2002) J. Cell Sci. 115:3007.
  2. Oh, R.S. et al. (2006) J. Biol. Chem. 281:21480.
  3. Burr, M.L. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2034.
  4. Sato, T. et al. (2012) Mol. Cell 47:99.
  5. Menon M.B., et.al. (2013) Biochem J. 456: 163.
  6. Burr M.L., et al. (2013) Proc Natl Acad Sci. 110: 14290.  
Long Name
Ubiquitin-conjugating Enzyme E2J 1
Entrez Gene IDs
51465 (Human); 56228 (Mouse); 297961 (Rat)
Alternate Names
CGI-76; EC 6.3.2.19; HSPC153; HSPC205; HSU93243; HsUBC6e; MGC12555; NCUBE1; NCUBE-1; NCUBE1HSUBC6e; Non-canonical ubiquitin-conjugating enzyme 1; non-canonical ubquitin conjugating enzyme 1; UBC6e; Ubc6p; UBE2J1; ubiquitin-conjugating enzyme E2 J1; ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast); Yeast ubiquitin-conjugating enzyme UBC6 homolog E

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