Ubiquitin-conjugating Enzyme E2, J2 (UBE2J2) is a 259 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of approximately 29 kDa. The human protein shares 100% aa sequence identity with the mouse and rat orthologs. UBE2J2 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). This enzyme has been reported to be involved in the ER-associated degradation pathway (2). UBE2J2 has also been shown to interact with the mK3 Ubiquitin ligase (E3) and promote the ubiquitination of hydroxylated residues on major histocompatibility complex class I heavy chains (3). This protein contains an N-terminal 6-His tag.
Recombinant Human His6-UBE2J2 Isoform 1 Protein, CF
R&D Systems | Catalog # E2-710
Discontinued Product
E2-710 has been discontinued.
View all UBE2J2 products.
Key Product Details
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human UBE2J2 protein
Contains an N-terminal 6-His tag
Contains an N-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
26 kDa
Activity
Recombinant Human His6-UBE2J2-1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2J2-1 concentration of 0.1-1 μM.
Formulation, Preparation, and Storage
E2-710
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: UBE2J2
References
- Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
- Kostova, Z. et al. (2007) Semin. Cell Dev. Biol. 18:770.
- Wang, X. et al. (2009) J. Cell Biol. 187:655.
Long Name
Ubiquitin-conjugating Enzyme E2, J2
Alternate Names
NCUBE2, PRO2121, Ubc6, Ubc6p
Gene Symbol
UBE2J2
UniProt
Additional UBE2J2 Products
Product Documents for Recombinant Human His6-UBE2J2 Isoform 1 Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human His6-UBE2J2 Isoform 1 Protein, CF
For research use only
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Associated Pathways
