Recombinant Human His6-UBE2J2 Isoform 1 Protein, CF
Recombinant Human His6-UBE2J2 Isoform 1 Protein, CF Summary
Contains an N-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Ubiquitin-conjugating Enzyme E2, J2 (UBE2J2) is a 259 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of approximately 29 kDa. The human protein shares 100% aa sequence identity with the mouse and rat orthologs. UBE2J2 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). This enzyme has been reported to be involved in the ER-associated degradation pathway (2). UBE2J2 has also been shown to interact with the mK3 Ubiquitin ligase (E3) and promote the ubiquitination of hydroxylated residues on major histocompatibility complex class I heavy chains (3). This protein contains an N-terminal 6-His tag.
- Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
- Kostova, Z. et al. (2007) Semin. Cell Dev. Biol. 18:770.
- Wang, X. et al. (2009) J. Cell Biol. 187:655.
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