Recombinant Human His6-UBE2J2 Isoform 1 Protein, CF

Catalog # Availability Size / Price Qty
E2-710-100
Product Details
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Recombinant Human His6-UBE2J2 Isoform 1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-UBE2J2-1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2J2-1 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2J2 protein
Contains an N-terminal 6-His tag
Accession #
Predicted Molecular Mass
26 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-710

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: UBE2J2

Ubiquitin-conjugating Enzyme E2, J2 (UBE2J2) is a 259 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of approximately 29 kDa. The human protein shares 100% aa sequence identity with the mouse and rat orthologs. UBE2J2 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). This enzyme has been reported to be involved in the ER-associated degradation pathway (2). UBE2J2 has also been shown to interact with the mK3 Ubiquitin ligase (E3) and promote the ubiquitination of hydroxylated residues on major histocompatibility complex class I heavy chains (3). This protein contains an N-terminal 6-His tag.

References
  1. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  2. Kostova, Z. et al. (2007) Semin. Cell Dev. Biol. 18:770.
  3. Wang, X. et al. (2009) J. Cell Biol. 187:655.
Long Name
Ubiquitin-conjugating Enzyme E2, J2
Entrez Gene IDs
118424 (Human); 140499 (Mouse); 298689 (Rat)
Alternate Names
EC 6.3.2.19; J2; NCUBE2; PRO2121; Ubc6; Ubc6p; UBE2J2; ubiquitin-conjugating enzyme E2 J2; ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast); yeast UBC6 homolog

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