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Recombinant Human His6-UBE2N/Ubc13 Protein, CF

Catalog #: E2-660
2 Citations Datasheet / COA / SDS

Discontinued Product

E2-660 has been discontinued.
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Recombinant Human His6-UBE2N/Ubc13 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-UBE2N/Ubc13 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2N/Ubc13 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2N/Ubc13 protein
Contains a 6-His tag
Accession #
Q969M7
Predicted Molecular Mass
19 kDa

Product Datasheets

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E2-660

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-660

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2N/Ubc13

Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), is a member of the Ubiquitin-conjugating (E2) enzyme family. UBE2N/Ubc13 has a predicted molecular weight of 17 kDa. The human enzyme shares 100% and 99% amino acid sequence identity with its mouse and rat orthologs, respectively. UBE2N/Ubc13 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). UBE2N/Ubc13 localizes to both the nucleus and cytoplasm (2). UBE2N/Ubc13 forms heterodimeric complexes with Uev1a/UBE2V1 and Mms2, both of which are catalytically inactive E2 enzyme variants. The UBE2N (Ubc13)/Uev1A Complex is found in the cytoplasm and is important for inflammatory responses via Nuclear Factor  kappa B (NF-kappa B) activation (3,4). In contrast, the UBE2N/Ubc13-Mms2 complex functions in the nucleus and is required for an efficient DNA damage response (3,5-7). Pathologically, UBE2N (Ubc13)/Uev1a Complex-mediated NF-kappa B activation is required for the proliferation of diffuse large B-cell lymphoma cells (8). This protein has an N-terminal His6-tag.

References
  1. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  2. Laine, A. et al. (2006) Mol. Cell. Biol. 26:8901.
  3. Andersen, P.L. et al. (2005) J. Cell Biol. 170:745.
  4. Pertel, T. et al. (2011) Nature 472:361.
  5. Zhao, G.Y. et al. (2007) Mol. Cell 25:663.
  6. Stewart, G.S. et al. (2009) Cell 136:420.
  7. Campbell, S.J. et al. (2012) J. Biol. Chem. 287:23900.
  8. Pulvino, M. et al. (2012) Blood 120:1668.
 
Entrez Gene IDs
7334 (Human); 93765 (Mouse); 116725 (Rat)
Alternate Names
bendless-like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; BLU; EC 6.3.2.19; MGC131857; MGC8489; UBC13; UbcH13; UbcH-ben; UBE2N; Ubiquitin carrier protein N; ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); Ubiquitin-protein ligase N; yeast UBC13 homolog

Citations for Recombinant Human His6-UBE2N/Ubc13 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing SWI/SNF
    Authors: Radko-Juettner, S;Yue, H;Myers, JA;Carter, RD;Robertson, AN;Mittal, P;Zhu, Z;Hansen, BS;Donovan, KA;Hunkeler, M;Rosikiewicz, W;Wu, Z;McReynolds, MG;Roy Burman, SS;Schmoker, AM;Mageed, N;Brown, SA;Mobley, RJ;Partridge, JF;Stewart, EA;Pruett-Miller, SM;Nabet, B;Peng, J;Gray, NS;Fischer, ES;Roberts, CWM;
    Nature
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Legionella pneumophila inhibits immune signalling via MavC-mediated transglutaminase-induced ubiquitination of UBE2N
    Authors: N Gan, ES Nakayasu, PJ Hollenbeck, ZQ Luo
    Nat Microbiol, 2018-11-12;4(1):134-143.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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