Recombinant Human His6-UBE2Q1 Isoform 1 Protein, CF

Catalog # Availability Size / Price Qty
E2-755-100
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Recombinant Human His6-UBE2Q1 Isoform 1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-UBE2Q1-1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2Q1-1 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2Q1 protein
Contains an N-terminal 6-His tag
Accession #
Predicted Molecular Mass
47 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-755

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol, 2 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Reconstitution Calculator

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Background: UBE2Q1

Ubiquitin-conjugating Enzyme E2 Q1 (UBE2Q1) is a widely expressed, 422 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of approximately 42.5 kDa (1). The human protein shares 99% aa sequence identity with the mouse and rat orthologs. UBE2Q1 has an RWD domain and an E2 catalytic core domain, the latter of which is required for the formation of a thioester bond with Ubiquitin (2,3). UBE2Q1 has been reported to regulate the internalization of the cell surface protein, GalT1 (4). Female UBE2Q1-deficient mice produce litters with increased embryonic lethality and have decreased implantation capacity of homozygous mutant embryos, suggesting that UBE2Q1 is important for female fertility (1). Additionally, UBE2Q1 mRNA and protein expression levels are increased in breast cancer samples compared to non-cancerous breast tissue samples, suggesting that UBE2Q1 may play a role in cancer (3). This protein contains an N-terminal 6-His tag.

References
  1. Grzmil, P. et al. (2012) Reproduction 145:45.
  2. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  3. Seghatoleslam, A. et al. (2012) Mol. Biol. Rep. 39:5135.
  4. Wassler, M.J. et al. (2008) Stem Cells 26:2006.
 
Long Name
Ubiquitin-conjugating Enzyme E2Q 1
Entrez Gene IDs
55585 (Human); 70093 (Mouse); 295252 (Rat)
Alternate Names
EC 6.3.2.19; galactosyl transferase-associated protein; GTAP; NICE5; NICE-5; PRO3094; Protein NICE-5; UBE2Q; UBE2Q1; Ubiquitin carrier protein Q1; ubiquitin-conjugating enzyme E2 Q1; ubiquitin-conjugating enzyme E2Q (putative) 1; ubiquitin-conjugating enzyme E2Q (putative); ubiquitin-conjugating enzyme E2Q family member 1; Ubiquitin-protein ligase Q1

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