Ubiquitin-conjugating Enzyme E2T (UBE2T), also known as HSPC150 Protein Similar to Ubiquitin-conjugating Enzyme, is a member of the Ubiquitin-conjugating (E2) enzyme family. UBE2T has a predicted molecular weight of 22.5 kDa. The human protein shares 83% and 80% amino acid sequence identity with the mouse and rat orthologs, respectively. UBE2T has an E2 catalytic core domain with an active site cysteine residue and undergoes auto-ubiquitination on Lys91 and either Lys182 or Lys191 (1,2). UBE2T localizes to the nucleus and nucleolus and plays an important role in the Fanconi anemia DNA repair pathway (1,3). More specifically, UBE2T interacts with the RING domain of FANCL to mediate the ubiquitination of FANCD2 on Lys563 following exposure to mitomycin C (2,4,5). UBE2T has also been linked to the nucleotide excision repair pathway in chicken DT40 cells (6). UBE2T expression is downregulated under hypoxic conditions, and it may play a role in the development and/or progression of breast cancer (3,7). This protein has an N-terminal His6-tag.
Recombinant Human His6-UBE2T Protein, CF
R&D Systems | Catalog # E2-695
Discontinued Product
E2-695 has been discontinued.
View all UBE2T products.
Key Product Details
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human UBE2T protein
Contains an N-terminal 6-His tag
Contains an N-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
24 kDa
Activity
Recombinant Human His6-UBE2T is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2T concentration of 0.1-1 μM.
Formulation, Preparation, and Storage
E2-695
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: UBE2T
References
- Machida, Y.J. et al. (2006) Mol. Cell 23:589.
- Alpi, A.F. et al. (2008) Mol. Cell 32:767.
- Ueki, T. et al. (2009) Cancer Res. 69:8752.
- Hodson, C. et al. (2011) J. Biol. Chem. 286:32628.
- Sato, K. et al. (2012) Nucleic Acids Res. 40:4553.
- Kelsall, I.R. et al. (2012) PLoS One 7:e36970.
- Ramaekers, C.H. et al. (2011) Radiother. Oncol. 101:190.
- Alpi A.F., et al. (2007) Mol. Cell Biol. 27: 8421.
- Hao J., et al. (2008) Tumour Biol. 29: 195.
- Longerich S., et al. (2009) J. Biol. Chem. 284: 23182.
- Martinex-Forero I., et al. (2009) Clin. Canc. Res. 15: 6751.
- Zhang J., et al. (2007) Cell. Cycle 7: 407.
- Zhang J., et al. (2007) J. Genet. Genomics. 34: 573.
Long Name
Ubiquitin-conjugating Enzyme E2T
Alternate Names
HSPC150, PIG50
Gene Symbol
UBE2T
UniProt
Additional UBE2T Products
Product Documents for Recombinant Human His6-UBE2T Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human His6-UBE2T Protein, CF
For research use only
Citations for Recombinant Human His6-UBE2T Protein, CF
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Associated Pathways
