Recombinant Human His6-UBE2T Protein, CF

Catalog # Availability Size / Price Qty
E2-695-100
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Recombinant Human His6-UBE2T Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-UBE2T is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2T concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2T protein
Contains an N-terminal 6-His tag
Accession #
Predicted Molecular Mass
24 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-695

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2T

Ubiquitin-conjugating Enzyme E2T (UBE2T), also known as HSPC150 Protein Similar to Ubiquitin-conjugating Enzyme, is a member of the Ubiquitin-conjugating (E2) enzyme family. UBE2T has a predicted molecular weight of 22.5 kDa. The human protein shares 83% and 80% amino acid sequence identity with the mouse and rat orthologs, respectively. UBE2T has an E2 catalytic core domain with an active site cysteine residue and undergoes auto-ubiquitination on Lys91 and either Lys182 or Lys191 (1,2). UBE2T localizes to the nucleus and nucleolus and plays an important role in the Fanconi anemia DNA repair pathway (1,3). More specifically, UBE2T interacts with the RING domain of FANCL to mediate the ubiquitination of FANCD2 on Lys563 following exposure to mitomycin C (2,4,5). UBE2T has also been linked to the nucleotide excision repair pathway in chicken DT40 cells (6). UBE2T expression is downregulated under hypoxic conditions, and it may play a role in the development and/or progression of breast cancer (3,7). This protein has an N-terminal His6-tag.

References
  1. Machida, Y.J. et al. (2006) Mol. Cell 23:589.
  2. Alpi, A.F. et al. (2008) Mol. Cell 32:767.
  3. Ueki, T. et al. (2009) Cancer Res. 69:8752.
  4. Hodson, C. et al. (2011) J. Biol. Chem. 286:32628.
  5. Sato, K. et al. (2012) Nucleic Acids Res. 40:4553.
  6. Kelsall, I.R. et al. (2012) PLoS One 7:e36970.
  7. Ramaekers, C.H. et al. (2011) Radiother. Oncol. 101:190.
  8. Alpi A.F., et al. (2007) Mol. Cell Biol. 27: 8421.
  9. Hao J., et al. (2008) Tumour Biol. 29: 195.
  10. Longerich S., et al. (2009) J. Biol. Chem. 284: 23182.
  11. Martinex-Forero I., et al. (2009) Clin. Canc. Res. 15: 6751.
  12. Zhang J., et al. (2007) Cell. Cycle 7: 407.
  13. Zhang J., et al. (2007) J. Genet. Genomics. 34: 573.          
 
Long Name
Ubiquitin-conjugating Enzyme E2T
Entrez Gene IDs
29089 (Human); 67196 (Mouse); 360847 (Rat)
Alternate Names
Cell proliferation-inducing gene 50 protein; EC 6.3.2.19; HSPC150 protein similar to ubiquitin-conjugating enzyme; HSPC150; PIG50; UBE2T; Ubiquitin carrier protein T; ubiquitin conjugating enzyme; ubiquitin-conjugating enzyme E2 T; ubiquitin-conjugating enzyme E2T (putative); Ubiquitin-protein ligase T

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