Recombinant Human His6-USP1 Protein, CF

Catalog # Availability Size / Price Qty
E-564-050
Product Details
Citations (1)
FAQs
Reviews

Recombinant Human His6-USP1 Protein, CF Summary

Product Specifications

Purity
>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human USP1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1 concentration of 0.01-0.5 μM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP1 protein
Contains substitutions at G670A & G671A and a C-terminal 6-His tag
Accession #
Predicted Molecular Mass
89 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-564

Formulation

X mg/ml (X μM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: USP1

USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway. Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA. USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. By itself, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains. This recombinant protein contains a C-terminal 6-His tag, and glycine-to-alanine substitutions at positions 670 and 671 (UniProt O94782 numbering).

References
  1. Cohn M.A., et al. (2007) Mol. Cell 28:786-797
  2. Huang T.T., et al. (2006) Nat. Cell Biol. 8: 339-347
  3. Nijman S.M.B., et al. (2005) Mol. Cell 17:331-339
  4. Williams S.A, et al. (2011) Cell 146: 918–930
Long Name
Ubiquitin Specific Protease 1
Entrez Gene IDs
7398 (Human); 230484 (Mouse); 313387 (Rat)
Alternate Names
Deubiquitinating Enzyme 1; EC 3.1.2.15; EC 3.4.19.12; hUBP; ubiquitin carboxyl terminal hydrolase 1; Ubiquitin Carboxyl-Terminal Hydrolase 1; ubiquitin specific peptidase 1; ubiquitin specific processing protease 1; ubiquitin specific protease 1; Ubiquitin Thioesterase 1; Ubiquitin thiolesterase 1; Ubiquitin-specific-processing protease 1; UBP; USP1

Citation for Recombinant Human His6-USP1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase
    Authors: M Fottner, AD Brunner, V Bittl, D Horn-Ghetk, A Jussupow, VRI Kaila, A Bremm, K Lang
    Nat. Chem. Biol., 2019;15(3):276-284.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human His6-USP1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human His6-USP1 Protein, CF and earn rewards!

Have you used Recombinant Human His6-USP1 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review