Recombinant Human His6-USP1 Protein, CF

R&D Systems | Catalog # E-564

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human His6-USP1 Protein (E-564)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

O94782.1

Applications

Bioactivity
View all USP1 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP1 protein
Met1 - Leu785 (Gly670Ala & Gly671Ala) with a C-terminal 6-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

89 kDa

Activity

Recombinant Human USP1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1 concentration of 0.01-0.5 μM.

Formulation, Preparation, and Storage

E-564
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: USP1

USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway. Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA. USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. By itself, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains. 

References

  1. Cohn, M.A., et al. (2007) Mol. Cell 28:786.
  2. Huang, T.T., et al. (2006) Nat. Cell Biol. 8:339.
  3. Nijman, S.M.B., et al. (2005) Mol. Cell 17:331.
  4. Williams, S.A, et al. (2011) Cell 146:918.

Long Name

Ubiquitin Specific Protease 1

Alternate Names

Deubiquitinating Enzyme 1, Ubiquitin Carboxyl-Terminal Hydrolase 1, Ubiquitin Thioesterase 1, UBP

Entrez Gene IDs

7398 (Human); 230484 (Mouse); 313387 (Rat)

Gene Symbol

USP1

UniProt

O94782.1

Additional USP1 Products

Product Documents for Recombinant Human His6-USP1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human His6-USP1 Protein, CF

For research use only

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Recombinant Human His6-USP1 Protein, CF

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