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Recombinant Human His6-USP22 Protein, CF

Catalog #: E-608 Datasheet / COA / SDS
 
Catalog # Availability Size / Price Qty
E-608-050
R&D Systems Recombinant Proteins and Enzymes
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-USP22 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant human USP22 is a Ubiquitin-specific deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial USP22 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) substrates.  Note: Recombinant USP22 is not active toward poly-Ubiquitin chains, Ubiquitinated histones and other Ubiquitinated protein substrates in the absence of adapter proteins such as ATXN7L or ENY2.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP22 protein
Contains an N-terminal 6-His tag
Accession #
Q9UPT9
Predicted Molecular Mass
59 kDa

Product Datasheets

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E-608

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-608

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, and 2 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP22

Ubiquitin carboxyl-terminal hydrolase 22 (USP22) is a specialized cysteine protease with a predicted molecular weight of 60 kDa. USP22 is a member of the peptidase C19 family (UBP8 subfamily) and the human protein shares 98% amino acid sequence identity with its mouse ortholog. USP22 is a member of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, a 2 MDa protein machine that mediates the acetylation and deubiquitination of histones as well as non-histone substrates. Within SAGA, USP22 binds directly with ATXN7L3 and provides the activity required to deubiquitinate mono-Ubiquitinated histone H2B. Recent findings suggest USP27X and USP51, which function independently of SAGA, may compete with USP22 for ATXN7L3 and ENY2 binding, and that imbalances in these activities may contribute to human diseases including cancer.

References
  1. Atanassov B.S. et al. (2016) Mol. Cell 62:558.
  2.  Atanassov B.S. et al. (2011) FEBS Lett. 585:2016.
  3. Atanassov B.S. & Dent S.R. (2016) personal communication.
  4. Koutelou E. et al. (2010) Curr Opin Cell Biol. 22:374.
  5. Lang G. et al. (2011) Mol Cell Biol. 31:3734.
  6.  Zhang X.Y. et al. (2008) Mol. Cell 29:102.
  7. Zhao Y. et al. (2008) Mol. Cell 29:92.
Long Name
Ubiquitin Specific Protease 22
Entrez Gene IDs
23326 (Human); 216825 (Mouse); 303201 (Rat)
Alternate Names
Deubiquitinating enzyme 22; EC 3.1.2.15; EC 3.4.19.12; KIAA1063ubiquitin carboxyl-terminal hydrolase 22; KIAA1064; ubiquitin specific peptidase 22; ubiquitin specific peptidase 3-like; ubiquitin specific protease 22; Ubiquitin Thioesterase 22; Ubiquitin thiolesterase 22; ubiquitin-specific processing protease 22; Ubiquitin-specific-processing protease 22; USP22; USP3L

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