Recombinant Human His6-USP22 Protein, CF
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Recombinant Human His6-USP22 Protein, CF Summary
Product Specifications
Val2 - Glu525 with a N-terminal 6-His tag
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-608
| Formulation | Supplied as a solution in HEPES, NaCl, Glycerol and TCEP. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: USP22
Ubiquitin carboxyl-terminal hydrolase 22 (USP22) is a 60 kD specialized Ubiquitin-specific deconjugating cysteine protease. USP22 is a member of the peptidase C19 family (UBP8 subfamily) and the human protein shares 98% amino acid sequence identity with its mouse ortholog. USP22 is a member of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, a 2 MDa protein machine that mediates the acetylation and deubiquitination of histones as well as non-histone substrates. Within SAGA, USP22 binds directly with ATXN7L3 and provides the activity required to deubiquitinate mono-Ubiquitinated histone H2B. Recombinant USP22 is not active toward poly-Ubiquitin chains, Ubiquitinated histones and other Ubiquitinated protein substrates in the absence of adapter proteins such as ATXN7L or ENY2. Recent findings suggest USP27X and USP51, which function independently of SAGA, may compete with USP22 for ATXN7L3 and ENY2 binding, and that imbalances in these activities may contribute to human diseases including cancer.
- Atanassov B.S. et al. (2016) Mol. Cell 62:558.
- Atanassov B.S. et al. (2011) FEBS Lett. 585:2016.
- Atanassov B.S. & Dent S.R. (2016) personal communication.
- Koutelou E. et al. (2010) Curr Opin Cell Biol. 22:374.
- Lang G. et al. (2011) Mol Cell Biol. 31:3734.
- Zhang X.Y. et al. (2008) Mol. Cell 29:102.
- Zhao Y. et al. (2008) Mol. Cell 29:92.
Citation for Recombinant Human His6-USP22 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Accelerating inhibitor discovery for deubiquitinating enzymes
Authors: WC Chan, X Liu, RS Magin, NM Girardi, SB Ficarro, W Hu, MI Tarazona G, CA Starnbach, A Felix, G Adelmant, AC Varca, B Hu, AS Bratt, E DaSilva, NJ Schauer, I Jaen Maiso, EK Dolen, AX Ayala, JA Marto, SJ Buhrlage
Nature Communications, 2023-02-08;14(1):686.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay
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