Recombinant Human His6-USP7 Protein, CF

Catalog # Availability Size / Price Qty
E-519-025
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Recombinant Human His6-USP7 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-USP7 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-USP7 concentration of 1-5 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP7 protein
Contains an N-terminal 6-His tag
Accession #
Predicted Molecular Mass
129 kDa

Product Datasheets

E-519

Formulation

X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: USP7

Ubiquitin Specific Peptidase 7 (USP7), also known as Herpes Virus-associated Ubiquitin Specific Protease (HAUSP), is a widely expressed deubiquitinating enzyme belonging to the peptidase C19 family (1). It has a predicted molecular weight of 130 kDa (2). Human USP7 is 1102 amino acids (aa) in length and shares 99% aa sequence identity with the mouse and rat orthologs (2,3). USP7 consists of a cysteine peptidase core (aa 208-560) that is flanked by an N-terminal TRAF-like domain (aa 50-205) and two C-terminal protease-resistant domains (aa 622-801 and 885-1061) (2,3). USP7 can be phosphorylated at Ser18 and Ser963 and ubiquitinated at Lys869 (2,4). USP7 was initially identified as a p53-interacting protein that deubiquitinates p53, thereby stabilizing the protein and inducing p53-dependent cell growth arrest and apoptosis (5). USP7 also targets the p53 regulatory proteins MDM2, MDMX, and Daxx, the epigenetic regulator Histone 2B, and the transcription factor FoxO4 (4,6-10). Additionally, USP7 interacts with the HSV-1 immediate early protein ICP0, contributing to the stabilization and transactivation capability of ICP0 during HSV-1 infection (1,11,12).

References
  1. Everett, R.D. et al. (1997) EMBO J. 16:1519.
  2. Fernández-Montalván, A. et al. (2007) FEBS J. 274:4256.
  3. Holowaty, M.N. et al. (2003) J. Biol. Chem. 278:47753.
  4. Khoronenkova, S.V. et al. (2012) Mol. Cell 45:801.
  5. Li, M. et al. (2002) Nature 416:648.
  6. Brooks, C.L. & W. Gu (2004) Cell Cycle 3:895.
  7. Meulmeester, E. et al. (2005) Cell Cycle 4:1166.
  8. Tanga, J. et al. (2010) Biochem. Biophys. Res. Commun. 393:542.
  9. van der Knaap, J.A. et al. (2005) Mol. Cell 17:695.
  10. van der Horst, A. et al. (2006) Nat. Cell Biol. 8:1043.
  11. Everett, R.D. et al. (1999) J. Virol. 73:417.
  12. Boutell, C. et al. (2005) J. Virol. 79:12342.
Long Name
Ubiquitin Specific Protease 7
Entrez Gene IDs
7874 (Human); 252870 (Mouse); 360471 (Rat)
Alternate Names
Deubiquitinating enzyme 7; EC 3.1.2.15; EC 3.4.19.12; HAUSP; HAUSPTEF1; Herpes virus-associated ubiquitin-specific protease; Herpesvirus-associated ubiquitin-specific protease; TEF1; ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific peptidase 7 (herpes virus-associated); ubiquitin specific protease 7 (herpes virus-associated); Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7; USP7

Citations for Recombinant Human His6-USP7 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Regulation of transcriptional activators by DNA-binding domain ubiquitination
    Authors: V Landré, B Revi, MG Mir, C Verma, TR Hupp, N Gilbert, KL Ball
    Cell Death Differ., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  2. Discovery and characterization of highly potent and selective allosteric USP7 inhibitors
    Authors: G Gavory, CR O'Dowd, MD Helm, J Flasz, E Arkoudis, A Dossang, C Hughes, E Cassidy, K McClelland, E Odrzywol, N Page, O Barker, H Miel, T Harrison
    Nat. Chem. Biol., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  3. DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization.
    Authors: Papp, Stephani, Huber, Anne-Lau, Jordan, Sabine D, Kriebs, Anna, Nguyen, Madelena, Moresco, James J, Yates, John R, Lamia, Katja A
    Elife, 2015;4(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses.
    Authors: Liang, Qin, Dexheimer, Thomas S, Zhang, Ping, Rosenthal, Andrew S, Villamil, Mark A, You, Changjun, Zhang, Qiuting, Chen, Junjun, Ott, Christin, Sun, Hongmao, Luci, Diane K, Yuan, Bifeng, Simeonov, Anton, Jadhav, Ajit, Xiao, Hui, Wang, Yinsheng, Maloney, David J, Zhuang, Zhihao
    Nat Chem Biol, 2014;10(4):298-304.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay

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Recombinant Human His6-USP7 Protein, CF
By Anonymous on 12/05/2018
Application: Enzymatic activity in vitro