Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF

Catalog # Availability Size / Price Qty
SP-600-100
Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein SDS-PAGE
1 Image
Product Details
Citations (1)
FAQs
Reviews

Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
This protein is useful as a substrate in in vitro assays using recombinant E3 Ubiquitin ligases and small molecule degraders. Reaction conditions will need to be optimized for each specific application.
Source
E. coli-derived human BRD4 protein
Glu49 - Glu460 with N-terminal 8-His and (DYKDDDDK) tags

Accession #
Predicted Molecular Mass
52 kDa

Product Datasheets

You must select a language.

x

SP-600

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

SP-600

Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Scientific Data

SDS-PAGE Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein SDS-PAGE View Larger

1 μg per lane of Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460 (Catalog # SP-600) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: BRD4

Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory.  BRD4 has two N-terminal bromodomains and one NET (N-terminal Extra Terminal) domain.  BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II.  BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.  This recombinant protein contains an N-terminal portion of BRD4 including both bromodomains. This protein is useful as a substrate in in vitro assays using recombinant E3 Ubiquitin ligases and small molecule degraders.

References
  1. Devaiah, B.N. et al. (2012) Proc. Natl. Acad. Sci. 109:6927.
  2. Gadd, M.S. et al. (2017) Nat. Chem. Biol. 13:514.
  3. Wang, R. et al. (2012) J. Biol. Chem. 287:10738.
  4. Zhang, W. et al. (1994) J. Biol. Chem. 287:43137.
Long Name
Bromodomain Containing 4
Entrez Gene IDs
23476 (Human); 57261 (Mouse)
Alternate Names
BRD4; bromodomain containing 4; bromodomain-containing 4; CAPchromosome-associated protein; HUNK1; HUNK1bromodomain-containing protein 4; HUNKI; MCAP; Protein HUNK1

Citation for Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Targeted Protein Degradation through Recruitment of the CUL4A Complex Adaptor Protein DDB1
    Authors: Meyers, M;Cismoski, S;Panidapu, A;Chie-Leon, B;Nomura, DK;
    bioRxiv : the preprint server for biology
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF and earn rewards!

Have you used Recombinant Human His8-(DYKDDDDK)-BRD4 (49-460) Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review