Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Recombinant Human IFN-alpha A (alpha 2a) Protein
R&D Systems | Catalog # 11100-1
New Version Available:
10984-IF
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human IFN-alpha A (alpha 2a) Protein (11100-1)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived human IFN-alpha A protein
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
19.2 kDa
Activity
Interferon was titrated with the use of the cytopathic effect inhibition assay as described:
Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.
Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc.Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml.
The units are determined by use of a Human IFN Alpha A (Hu-IFN- alpha A [2a]) laboratory standard calibrated to the international reference standard for human interferon alpha A (Hu-IFN- alpha A [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257 (1-2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].
Reviewed Applications
Read 1 review rated 4 using 11100-1 in the following applications:
Formulation, Preparation, and Storage
11100-1
| Formulation | Phosphate, buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Background: IFN-alpha 2/IFNA2
Long Name
Interferon alpha 2
Alternate Names
Ifa2, IFNA2, IFNA2a, IFNA2b, IFNA2c, IFNAA, IFNalpha 2
Gene Symbol
IFNA2
UniProt
Additional IFN-alpha 2/IFNA2 Products
Product Documents for Recombinant Human IFN-alpha A (alpha 2a) Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human IFN-alpha A (alpha 2a) Protein
For research use only
Related Research Areas
Citations for Recombinant Human IFN-alpha A (alpha 2a) Protein
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Associated Pathways
NOD-like Receptor Signaling Pathways
Toll-Like Receptor Signaling Pathways
