Recombinant Human IFN-alpha A (alpha 2a) Protein

  (7 citations)   
Product Details
Citations (7)
FAQs
Reviews
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
  • Activity

    Interferon was titrated with the use of the cytopathic effect inhibition assay as described:

    Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.

    Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc.Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml.

    The units are determined by use of a Human IFN Alpha A (Hu-IFN- alpha A [2a]) laboratory standard calibrated to the international reference standard for human interferon alpha A (Hu-IFN- alpha A [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257 (1-2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].

  • Source
    E. coli-derived
  • Accession #
  • Predicted Molecular Mass
    19.2 kDa
11100-1
 
Formulation

Phosphate, buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: IFN-alpha A
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
  • Long Name:
    Interferon alpha A
  • Entrez Gene IDs:
    3440 (Human)
  • Alternate Names:
    IFNA2a; IFNalpha A; IFN-alpha A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Species
Applications
Sample Type
  1. Prevalence and correlation of cytokine-specific autoantibodies with epidemiological factors and C-reactive protein in 8,972 healthy individuals: Results from the Danish Blood Donor Study
    Authors: JH von Steman, AS Rigas, LW Thørner, DGK Rasmussen, OB Pedersen, K Rostgaard, C Erikstrup, H Ullum, MB Hansen
    PLoS ONE, 2017;12(6):e0179981.
    Species: Human
    Sample Type: Plasma
    Application: Ctrl
  2. HIV-1-Mediated BAFF Secretion in Macrophages Does Not Require Endosomal TLRs, Type-I IFN, and Nef, but Depends on the Cellular Phenotype Status
    Authors: AM Gomez, M Ouellet, A Deshiere, Y Breton, MJ Tremblay
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Rabex-5 is a lenalidomide target molecule that negatively regulates TLR-induced type 1 IFN production
    Proc Natl Acad Sci USA, 2016;113(38):10625-10630.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. HIV-1-triggered release of type I IFN by plasmacytoid dendritic cells induces BAFF production in monocytes.
    Authors: Gomez A, Ouellet M, Tremblay M
    J Immunol, 2015;194(5):2300-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Epigenetic reprogramming of the type III interferon response potentiates antiviral activity and suppresses tumor growth.
    Authors: Ding S, Khoury-Hanold W, Iwasaki A, Robek M
    PLoS Biol, 2014;12(1):e1001758.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. IFN-alpha induces transcription of hypoxia-inducible factor-1alpha to inhibit proliferation of human endothelial cells.
    Authors: Gerber SA, Pober JS
    J. Immunol., 2008;181(2):1052-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Have you used Recombinant Human IFN-alpha A (alpha 2a) Protein?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

R&D Systems Guarantee