>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Interferon was titrated with the use of the cytopathic effect inhibition assay as
– performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this
assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.
– performed as described [Budd, et al. (1985) Canc.Chem. Pharm. 12:39]. The EC50 for interferon
in this assay is ~1 U/ml.
The units are determined by use of a Human IFN Alpha A (Hu-IFN-
alpha A [2a]) laboratory standard calibrated to the
international reference standard for human interferon alpha A (Hu-IFN-
alpha A [2a]) provided by the National Institutes of
et al. (2001) J. Immunol Methods. 257 (1-2):17-33]. Please note that IFN assays vary between labs and
assay systems [Meager,
et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].
Phosphate, buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: IFN-alpha A
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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