Recombinant Human IFN-alpha A (alpha 2a) Protein

Catalog # Availability Size / Price Qty
11100-1
Product Details
Citations (8)
FAQs
Reviews

Recombinant Human IFN-alpha A (alpha 2a) Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Interferon was titrated with the use of the cytopathic effect inhibition assay as described:

Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.

Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc.Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml.

The units are determined by use of a Human IFN Alpha A (Hu-IFN- alpha A [2a]) laboratory standard calibrated to the international reference standard for human interferon alpha A (Hu-IFN- alpha A [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257 (1-2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].

Source
E. coli-derived human IFN-alpha A protein
Accession #
Predicted Molecular Mass
19.2 kDa

Product Datasheets

11100-1

Formulation

Phosphate, buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IFN-alpha A

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name
Interferon alpha A
Entrez Gene IDs
3440 (Human)
Alternate Names
IFNA2a; IFNalpha A; IFN-alpha A

Citations for Recombinant Human IFN-alpha A (alpha 2a) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Prevalence and correlation of cytokine-specific autoantibodies with epidemiological factors and C-reactive protein in 8,972 healthy individuals: Results from the Danish Blood Donor Study
    Authors: JH von Steman, AS Rigas, LW Thørner, DGK Rasmussen, OB Pedersen, K Rostgaard, C Erikstrup, H Ullum, MB Hansen
    PLoS ONE, 2017;12(6):e0179981.
    Species: Human
    Sample Types: Plasma
    Applications: Ctrl
  2. Rabex-5 is a lenalidomide target molecule that negatively regulates TLR-induced type 1 IFN production
    Proc Natl Acad Sci USA, 2016;113(38):10625-10630.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. HIV-1-Mediated BAFF Secretion in Macrophages Does Not Require Endosomal TLRs, Type-I IFN, and Nef, but Depends on the Cellular Phenotype Status
    Authors: AM Gomez, M Ouellet, A Deshiere, Y Breton, MJ Tremblay
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. HIV-1-triggered release of type I IFN by plasmacytoid dendritic cells induces BAFF production in monocytes.
    Authors: Gomez A, Ouellet M, Tremblay M
    J Immunol, 2015;194(5):2300-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Epigenetic reprogramming of the type III interferon response potentiates antiviral activity and suppresses tumor growth.
    Authors: Ding S, Khoury-Hanold W, Iwasaki A, Robek M
    PLoS Biol, 2014;12(1):e1001758.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. IFN-alpha induces transcription of hypoxia-inducible factor-1alpha to inhibit proliferation of human endothelial cells.
    Authors: Gerber SA, Pober JS
    J. Immunol., 2008;181(2):1052-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. PD-L1 is induced in hepatocytes by viral infection and by interferon-alpha and -gamma and mediates T cell apoptosis.
    Authors: Muhlbauer M, Fleck M, Schutz C, Weiss T, Froh M, Blank C, Scholmerich J, Hellerbrand C
    J. Hepatol., 2006;45(4):520-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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