Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

R&D Systems | Catalog # 11574-AB

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein (11574-AB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Structure / Form

Disulfide linked homodimer

Applications

Bioactivity
View all IFN-alpha/beta R1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IFN-alpha/beta R1 protein
Human IFNAR1
(Lys28-Lys436)
Accession # AAA52730.1		 GGIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

74 kDa

SDS-PAGE

105-120 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.

Recombinant Human IFN-alpha/beta R1 Fc Chimera (Catalog # 11574-AB) binds Recombinant Human LRPAP (Catalog # 4296-LR) with an ED50 of 0.750-9.00 μg/mL.

Scientific Data Images for Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein Binding Activity.

Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein (Catalog # 11574-AB) binds Recombinant Human LRPAP (4296-LR) with an ED50 of 0.750-9.00 μg/mL.

Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein (Catalog # 11574-AB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-120 kDa and 210-240 kDa, respectively.

Formulation, Preparation, and Storage

11574-AB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha/beta R1

Interferon‑alpha/beta receptor 1 (IFN‑ alpha / beta  R1), also known as IFNAR1, is a 100‑130 kDa member of the class II cytokine receptor family of proteins. These proteins form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1). IFN‑ alpha / beta  R1, in association with IFN‑ alpha / beta  R2, is required for propagating anti‑microbial signal transduction triggered by the type 1 interferons such as IFN‑ alpha and IFN‑ beta (2, 3). Mature human IFN‑ alpha / beta  R1 consists of a 409 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 100 aa cytoplasmic domain (4). The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. Within the ECD, human IFN‑ alpha / beta  R1 shares 47% and 50% aa identity with mouse and rat  IFN‑ alpha / beta  R1, respectively. Alternative splicing generates two additional isoforms that lack the transmembrane segment and either all or a portion of the cytoplasmic domain. IFN‑ alpha / beta  R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN‑ alpha / beta  R2. Ligands preferentially associate with IFN‑ alpha / beta  R2, and this complex subsequently forms a stable ternary assembly with IFN‑ alpha / beta  R1 (5‑7). IFN‑ alpha / beta  R1 also associates with IFN‑ gamma  R2 even in the absence of IFN‑ gamma stimulation (3). IFN‑ alpha / beta  R1 activation depends on tyrosine phoshorylation as well as palmitoylation of its cytoplasmic domain (8, 9). Rapid down‑regulation of the receptor is accomplished by ligand‑dependent or ‑independent pathways (e.g. VEGF R signaling, TLR signaling, or cellular stress) which induce its serine phosphorylation, ubiquitination, and degradation (10‑13).

References

  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Hwang, S.Y. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11284.
  3. Takaoka, A. et al. (2000) Science 288:2357.
  4. Uze, G. et al. (1990) Cell 60:225.
  5. Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
  6. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
  7. Kalie, E. et al. (2008) J. Biol. Chem. 283:32925.
  8. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
  9. Claudinon, J. et al. (2009) J. Biol. Chem. 284:24328.
  10. Zheng, H. et al. (2011) Blood 118:4003.
  11. Qian, J. et al. (2011) PLoS Pathogens 7:e1002065.
  12. Bhattacharya, S. et al. (2010) J. Biol. Chem. 285:2318.
  13. Bhattacharya, S. et al. (2011) J. Biol. Chem. 286:22069.

Long Name

Interferon alpha/beta Receptor 1

Alternate Names

IFN-aR1, IFN-bR1, IFNAR1, IFNbR1

Entrez Gene IDs

3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)

Gene Symbol

IFNAR1

Additional IFN-alpha/beta R1 Products

Product Documents for Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human IFN-alpha/beta R1 Fc Chimera Protein, CF

For research use only

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Associated Pathways

Jak/STAT Signaling Pathway Jak/STAT Signaling Pathway Thumbnail