Recombinant Human LRPAP Protein, CF Summary
Tyr35-Leu357, with an N-terminal Met and a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
LRPAP (LDL receptor-related protein-associated protein 1; also named RAP), is a ubiquitously expressed 39 kDa chaperone for LDL receptor family proteins (1, 2). Mature human LRPAP shares 77% amino acid sequence identity with mouse and rat LRPAP. It is organized into three domains of comparable length. Domains D2 and D3 interact with each other, while D1 is independent (3). The D1 domain contains a low affinity binding site for LRP, and the associated D2 and D3 domains bind LRP with high affinity (4). The majority of LRPAP is localized in the endoplasmic reticulum and Golgi (5). LRPAP prevents the premature interaction of LRP, LRP2/megalin, and VLDLR with their coexpressed ligands, thereby promoting proper receptor folding and export from the ER (6 - 8). Protonation of conserved histidine residues within the D3 domain induces the separation of LRPAP and LRP in the relatively acidic Golgi (9). LRPAP, which contains a C-terminal HNEL motif, can then recycle to the ER (9). A minor amount of LRPAP remains associated with LRP and can modulate receptor activity on the cell surface (5). Exogenously applied LRPAP competitively inhibits LDL receptor family binding and uptake of activated alpha 2-macroglobulin, apoB100- or apoE-enriched LDL and VLDL particles, cholesteryl esters, and complexes of PAI-1 with either tPA or uPA (10 - 14).
- Strickland, D.K. et al. (1991) J. Biol. Chem. 266:13364.
- Bu, G. (2001) Int. Rev. Cytol. 209:79.
- Lazic, A. et al. (2003) Biochemistry 42:14913.
- Lazic, A. et al. (2006) Arch. Biochem. Biophys. 450:167.
- Bu, G. et al. (1994) J. Biol. Chem. 269:29874.
- Willnow, T.E. et al. (1996) EMBO J. 15:2632.
- Bu, G. and S. Rennke (1996) J. Biol. Chem. 271:22218.
- Obermoeller, L.M. et al. (1997) J. Biol. Chem. 272:10761.
- Lee, D. et al. (2006) Mol. Cell 22:423.
- Williams, S.E. et al. (1992) J. Biol. Chem. 267:9035.
- Medh, J.D. et al. (1995) J. Biol. Chem. 270:536.
- Herz, J. et al. (1991) J. Biol. Chem. 266:21232.
- Mokuno, H. et al. (1994) J. Biol. Chem. 269:13238.
- Orth, K. et al. (1992) Proc. Natl. Acad. Sci. 89:7422.
Citations for Recombinant Human LRPAP Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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The amyloid-beta degradation intermediate Abeta34 is pericyte-associated and reduced in brain capillaries of patients with Alzheimer's disease
Authors: T Kirabali, S Rigotti, A Siccoli, F Liebsch, A Shobo, C Hock, RM Nitsch, G Multhaup, L Kulic
Acta Neuropathol Commun, 2019;7(1):194.
Sample Types: Whole Cells
Mapping the binding region on the low density lipoprotein receptor for blood coagulation factor VIII.
Authors: Kurasawa J, Shestopal S, Karnaukhova E, Struble E, Lee T, Sarafanov A
J Biol Chem, 2013;288(30):22033-41.
Sample Types: Protein
Applications: Binding Assay
HIV-1 promotes intake of Leishmania parasites by enhancing phosphatidylserine-mediated, CD91/LRP-1-dependent phagocytosis in human macrophages.
Authors: Lodge R, Ouellet M, Barat C, Andreani G, Kumar P, Tremblay MJ
PLoS ONE, 2012;7(3):e32761.
Sample Types: Whole Cells
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