Recombinant Human IGSF9B Protein, CF

Catalog # Availability Size / Price Qty
9248-S9-050
Recombinant Human IGSF9B Protein, CF Data
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Recombinant Human IGSF9B Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 150 ng on a nitrocellulose-coated microplate.
Source
Human embryonic kidney cell, HEK293-derived human IGSF9B protein
Glu21-Pro722, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu21
Predicted Molecular Mass
79 kDa
SDS-PAGE
83-107 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9248-S9

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

Recombinant Human IGSF9B Inhibits Neurite Outgrowth in E13 Chick Embryonic Dorsal Root Ganglia (DRG) Neurons. A) PBS control coated nitrocellulose microplates showing E13 chick embryonic DRG neurite outgrowth. B) A 3 µl droplet, containing 150 ng of Recombinant Human IGSF9B (Catalog # 9248-S9) coated on nitrocellulose microplates, results in a significant reduction in E13 chick embryonic DRG neurite outgrowth.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGSF9B

IGSF9B, is an approximately 190 kDa transmembrane glycoprotein that plays a role in neuronal synapse maintenance and function (1). Mature human IGSF9B consists of a 702 amino acid (aa) extracellular domain (ECD) with 5 Ig-like domains and 2 fibronectin type-3 domains, a 21 aa transmembrane segment, and a 606 aa cytoplasmic domain. Within the ECD, human IGSF9B shares 97% aa sequence identity with mouse and rat IGSF9B. IGSF9B is widely expressed on interneurons in the brain where it localizes to inhibitory synapses in a ternary complex with Neuroligin-2 and S-SCAM (2). IGSF9B functions as a homophilic adhesion molecule and is required for the maturation of inhibitory synapses on interneurons (2). It shares 37% aa sequence identity with human IGSF9/Dasm1 which is expressed in the brain and dorsal root and trigeminal ganglia (3-6). Dasm1 also functions as a homophilic adhesion protein that supports the maintenance of inhibitory synapses as well as inhibitory neurotransmission (6, 7).

References
  1. Hansen, M. and P.S. Walmod (2013) Neurochem. Res. 38:1236.
  2. Woo, J. et al. (2013) J. Cell Biol. 201:929.
  3. Doudney, K. et al. (2002) Genomics 79:663.
  4. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13341.
  5. Mishra, A. et al. (2008) Mol. Cell. Biol. 28:2782.
  6. Mishra, A. et al. (2014) J. Neurosci. 34:4187.
  7. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13346.
Long Name
Immunoglobulin Superfamily, Member 9B
Entrez Gene IDs
22997 (Human); 235086 (Mouse); 315510 (Rat)
Alternate Names
IGSF9B; immunoglobulin superfamily, member 9B

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