>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 150 ng on a nitrocellulose-coated microplate.
Human embryonic kidney cell, HEK293-derived Glu21-Pro722, with a C-terminal 6-His tag
Recombinant Human IGSF9B Inhibits Neurite Outgrowth in E13Chick Embryonic Dorsal Root Ganglia (DRG) Neurons. A) PBS control coatednitrocellulose microplates showing E13 chick embryonic DRG neurite outgrowth.B) A 3 µl droplet, containing 150 ng of Recombinant Human IGSF9B (Catalog #9248-S9) coated on nitrocellulose microplates, results in a significantreduction in E13 chick embryonic DRG neurite outgrowth.
IGSF9B, is an approximately 190 kDa transmembrane glycoprotein that plays a role in neuronal synapse maintenance and function (1). Mature human IGSF9B consists of a 702 amino acid (aa) extracellular domain (ECD) with 5 Ig-like domains and 2 fibronectin type-3 domains, a 21 aa transmembrane segment, and a 606 aa cytoplasmic domain. Within the ECD, human IGSF9B shares 97% aa sequence identity with mouse and rat IGSF9B. IGSF9B is widely expressed on interneurons in the brain where it localizes to inhibitory synapses in a ternary complex with Neuroligin-2 and S-SCAM (2). IGSF9B functions as a homophilic adhesion molecule and is required for the maturation of inhibitory synapses on interneurons (2). It shares 37% aa sequence identity with human IGSF9/Dasm1 which is expressed in the brain and dorsal root and trigeminal ganglia (3-6). Dasm1 also functions as a homophilic adhesion protein that supports the maintenance of inhibitory synapses as well as inhibitory neurotransmission (6, 7).
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Doudney, K. et al. (2002) Genomics 79:663.
Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13341.
Mishra, A. et al. (2008) Mol. Cell. Biol. 28:2782.
Mishra, A. et al. (2014) J. Neurosci. 34:4187.
Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13346.
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