Recombinant Human IGSF9B Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 150 ng on a nitrocellulose-coated microplate.
  • Source
    Human embryonic kidney cell, HEK293-derived Glu21-Pro722, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Glu21
  • Predicted Molecular Mass
    79 kDa
  • SDS-PAGE
    83-107 kDa, reducing conditions
9248-S9
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human IGSF9B Inhibits Neurite Outgrowth in E13Chick Embryonic Dorsal Root Ganglia (DRG) Neurons. A) PBS control coatednitrocellulose microplates showing E13 chick embryonic DRG neurite outgrowth.B) A 3 µl droplet, containing 150 ng of Recombinant Human IGSF9B (Catalog #9248-S9) coated on nitrocellulose microplates, results in a significantreduction in E13 chick embryonic DRG neurite outgrowth.
Background: IGSF9B
IGSF9B, is an approximately 190 kDa transmembrane glycoprotein that plays a role in neuronal synapse maintenance and function (1). Mature human IGSF9B consists of a 702 amino acid (aa) extracellular domain (ECD) with 5 Ig-like domains and 2 fibronectin type-3 domains, a 21 aa transmembrane segment, and a 606 aa cytoplasmic domain. Within the ECD, human IGSF9B shares 97% aa sequence identity with mouse and rat IGSF9B. IGSF9B is widely expressed on interneurons in the brain where it localizes to inhibitory synapses in a ternary complex with Neuroligin-2 and S-SCAM (2). IGSF9B functions as a homophilic adhesion molecule and is required for the maturation of inhibitory synapses on interneurons (2). It shares 37% aa sequence identity with human IGSF9/Dasm1 which is expressed in the brain and dorsal root and trigeminal ganglia (3-6). Dasm1 also functions as a homophilic adhesion protein that supports the maintenance of inhibitory synapses as well as inhibitory neurotransmission (6, 7).
  • References:
    1. Hansen, M. and P.S. Walmod (2013) Neurochem. Res. 38:1236.
    2. Woo, J. et al. (2013) J. Cell Biol. 201:929.
    3. Doudney, K. et al. (2002) Genomics 79:663.
    4. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13341.
    5. Mishra, A. et al. (2008) Mol. Cell. Biol. 28:2782.
    6. Mishra, A. et al. (2014) J. Neurosci. 34:4187.
    7. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13346.
  • Long Name:
    Immunoglobulin Superfamily, Member 9B
  • Entrez Gene IDs:
    22997 (Human); 235086 (Mouse); 315510 (Rat)
  • Alternate Names:
    IGSF9B; immunoglobulin superfamily, member 9B
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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