Recombinant Human IL-13 R alpha 2 His-tag, CF

R&D Systems | Catalog # 11562-IR

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems HEK293-derived Recombinant Human IL-13 R alpha 2 His-tag (11562-IR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q14627.1

Applications

Bioactivity
View all IL-13 R alpha 2 Proteins and Enzymes »

Product Specifications

N-terminal Sequence Analysis

Pro 43

Predicted Molecular Mass

36 kDa

SDS-PAGE

53-58 kDa, under reducing conditions

Scientific Data Images for Recombinant Human IL-13 R alpha 2 His-tag, CF

Recombinant Human IL-13 R alpha 2 His-tag Protein Bioactivity.

Recombinant Human IL-13 R alpha 2 His-tag Protein (Catalog # 11562-IR) inhibits Recombinant Human IL-13 (213-ILB) induced proliferation in the TF-1 human erythroleukemic cells. The ED50 for this effect is 4.00-60.0 ng/mL.

Recombinant Human IL-13 R alpha 2 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL-13 R alpha 2 His-tag Protein (Catalog # 11562-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-58 kDa, under reducing conditions.

Formulation, Preparation, and Storage

11562-IR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-13 R alpha 2

Interleukin‑13 Receptor alpha 2 (IL‑13 R alpha 2), also known as IL‑13 binding protein, and CD213a2, is a widely expressed 55 kDa cytokine receptor that plays an important role in the Th2‑polarized immune responses characteristic of a variety of pathologies, including parasitic infections and allergic asthma (1, 2). Mature human IL‑13 R alpha 2 consists of a 317 amino acid (aa) extracellular domain with three fibronectin type‑III domains, a WSxWS motif, a 20 aa transmembrane segment, and a 17 aa cytoplasmic domain (3). Within the ECD, human IL‑13 R alpha 2 shares 64% and 62% aa sequence identity with mouse and rat IL‑13 R alpha 2, respectively. In both mouse and human, a 40 kDa‑50 kDa soluble form of IL‑13 R alpha 2 can be generated by MMP‑8 mediated shedding in vitro (4).  Although this is assumed to occur in vivo in mouse, there is no evidence that shedding occurs in human (5‑7).  In mouse, alternative splicing also leads to sIL‑13 R alpha 2, but again, this phenomenon apparently does not occur in human (6‑7).  Thus, the biological effects of human IL‑13 R alpha 2 would appear to be mediated exclusively by membrane IL‑13 R alpha 2 (7). The biological effects of IL‑13 and IL‑4 are closely related in part due to a shared receptor system. IL‑13 binds to IL‑13 R alpha 1 which then forms a signaling complex with IL‑4 R alpha (8, 9). IL‑13 R alpha 2 functions as a decoy receptor by binding and internalizing IL‑13 and preventing it from signaling through the IL‑13 R alpha 1/IL‑4 R alpha complex (3, 10). IL‑13 R alpha 2 can also block IL‑4 induced responses by inhibiting IL‑4 bound IL‑13 R alpha 1/IL‑4 R alpha receptor complexes even though it does not itself bind IL‑4 (11, 12). Aside from its decoy function, IL‑13‑activated IL‑13 R alpha 2 directly promotes the development of tissue fibrosis by inducing the transcription of TGF‑ beta (13). Presumably, any human soluble IL‑13 R alpha 2, if it exists, will retain its ligand binding capability and attenuate responses to IL‑13 but not to IL‑4 (11, 14). The up‑regulation of transmembrane during Th2‑biased immune responses limits the extent of those responses (15‑17).

References

  1. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  2. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  3. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
  4. Chen, W. et al. (2008) J. Allergy Clin. Immunol. 122:625.
  5. O’Toole, M. et al. (2008) Clin. Exp. Allergy 38:594.
  6. Chen, W. et al. (2009) J. Immunol. 183:7870.
  7. Kasaian, M.T. et al. (2011) J. Immunol. 187:561.
  8. Andrews, A.-L. et al. (2006) J. Immunol. 176:7456.
  9. Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.
  10. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
  11. Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  12. Rahaman, S.O. et al. (2002) Cancer Res. 62:1103.
  13. Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99.
  14. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
  15. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  16. Morimoto, M. et al. (2009) J. Immunol. 183:1934.
  17. Zheng, T. et al. (2008) J. Immunol. 180:522.

Long Name

Interleukin 13 Receptor alpha 2

Alternate Names

CD213a2, IL-13Ra2, IL13R alpha 2, IL13RA2

Entrez Gene IDs

3598 (Human); 16165 (Mouse)

Gene Symbol

IL13RA2

UniProt

Q14627.1

Additional IL-13 R alpha 2 Products

Product Documents for Recombinant Human IL-13 R alpha 2 His-tag, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-13 R alpha 2 His-tag, CF

For research use only

Customer Reviews for Recombinant Human IL-13 R alpha 2 His-tag, CF

There are currently no reviews for this product. Be the first to review Recombinant Human IL-13 R alpha 2 His-tag, CF and earn rewards!

Have you used Recombinant Human IL-13 R alpha 2 His-tag, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes