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Recombinant Human IL-13 Protein

R&D Systems | Catalog # 213-ILB

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human IL-13 Protein (213-ILB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

AAK53823

Applications

Bioactivity
View all IL-13 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-13 protein
Gly21-Asn132

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly21

Predicted Molecular Mass

12.3 kDa

SDS-PAGE

9 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.5-2.5 ng/mL.

Reviewed Applications

Read 12 reviews rated 4.9 using 213-ILB in the following applications:

Scientific Data Images for Recombinant Human IL-13 Protein

Recombinant Human IL‑13 Protein SEC-MALS.

Recombinant Human IL-13 Protein (Catalog # 213-ILB) has a molecular weight (MW) of 12.0 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Graph showing dose-dependent bioactivity of Human IL-13 protein

Bioactivity of Human IL-13 Protein

Recombinant human IL-13 (213-ILB) stimulates proliferation in the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.5-2.5 ng/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 213-ILB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 213-ILB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-13

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells (1‑3). Mature human IL-13 shares approximately 58% amino acid sequence identity with mouse and rat IL-13. Despite the low homology, it exhibits cross‑species activity between human, mouse, and rat (4). IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. On B cells, it promotes cellular activation, immunoglobulin class switching to IgE, and the up‑regulation of CD23/Fc epsilon RII (1, 5). IL-13 binds with low affinity to the transmembrane IL-13 R alpha 1 which then forms a signaling complex with the transmembrane IL-4 R alpha (6‑8). This high affinity receptor complex also functions as the type 2 IL-4 receptor (6, 7). IL‑13 R alpha 2 inhibits responses to both IL-13 and IL-4. It binds IL-13 with high affinity (9, 10) and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex (11, 12). It also blocks signaling through IL-4-occupied IL-13 R alpha 1/IL-4 R alpha receptor complexes (12, 13). In addition, IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis (14‑16).

References

  1. Saggini, A. et al. (2011) Int. J. Immunopathol. Pharmacol. 24:305.
  2. Gallo, E. et al. (2012) Eur. J. Immunol. 42:2322.
  3. Liang, H.E. et al. (2012) Nat. Immunol. 13:58.
  4. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  5. McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735.
  6. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  7. Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.
  8. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  9. Chen, W. et al. (2009) J. Immunol. 183:7870.
  10. Daines, M.O. et al. (2007) J. Allergy Clin. Immunol. 119:375.
  11. Kasaian, M.T. et al. (2011) J. Immunol. 187:561.
  12. Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  13. Rahaman, S.O. et al. (2002) Cancer Res. 62:1103.
  14. Fujisawa, T. et al. (2009) Cancer Res. 69:8678.
  15. Fujisawa, T. et al. (2011) Int. J. Cancer 131:344.
  16. Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99.

Long Name

Interleukin 13

Alternate Names

IL13, NC30, P600

Entrez Gene IDs

3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)

Gene Symbol

IL13

UniProt

AAK53823

Additional IL-13 Products

Product Documents for Recombinant Human IL-13 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 213-ILB (with carrier)
Download SDS 213-ILB/CF (carrier free)

Product Specific Notices for Recombinant Human IL-13 Protein

For research use only

Citations for Recombinant Human IL-13 Protein

Customer Reviews for Recombinant Human IL-13 Protein (12)

4.9 out of 5
12 Customer Ratings
5 Stars
92%
4 Stars
8%
3 Stars
0%
2 Stars
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1 Stars
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Showing  1 - 5 of 12 reviews Showing All
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  • Recombinant Human IL-13 Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 08/03/2022
    Downregulation of a given gene after IL-13 exposure detected by qPCR. Three independent experiments.
    Recombinant Human IL-13 Protein 213-ILB
  • Recombinant Human IL-13 Protein
    Name: Anonymous
    Application: Flow Cytometry
    Verified Customer | Posted 08/03/2022
    Control vs. rIL-13 for 6h
    Recombinant Human IL-13 Protein 213-ILB
  • Recombinant Human IL-13 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 08/03/2022
    Detecting target protein expression after 12h 1. Control 2. +10ng/mL IL-13
    Recombinant Human IL-13 Protein 213-ILB
  • Recombinant Human IL-13 Protein
    Name: Anonymous
    Application: Cell adherence junction integrity
    Verified Customer | Posted 12/05/2021
    Cell adherence junction integrity in in vitro cell culture.
    Recombinant Human IL-13 Protein 213-ILB
  • Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 08/30/2019
  • Recombinant Human IL-13 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 08/16/2019
  • Recombinant Human IL-13 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/09/2019
    Recombinant Human IL-13 Protein 213-ILB
  • Recombinant Human IL-13 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/09/2019
    Recombinant Human IL-13 Protein 213-ILB
  • Recombinant Human IL-13 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 11/16/2018
    Recombinant Human IL-13 Protein 213-ILB
  • Recombinant Human IL-13 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 10/07/2018
    Cells were supplied with the cytokinetreatments: 50 ng /ml GM-CSF and 10 ng/ ml1 of IL-13 to differentiate DC cells
    Recombinant Human IL-13 Protein 213-ILB
  • Recombinant Human IL-13 Protein
    Name: Danlee Enzler
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 04/02/2018
  • Recombinant Human IL-13 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/20/2016
    Recombinant Human IL-13 Protein 213-ILB

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