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Recombinant Human IL-17A (Human Cell-expressed) Protein

R&D Systems | Catalog # 7955-IL

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human IL-17A (Human Cell-expressed) Protein (7955-IL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q16552

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all IL-17/IL-17A Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-17/IL-17A protein
Gly24-Ala155

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

15.1 kDa (monomer)

SDS-PAGE

15-23 kDa, reducing conditions
28-38 kDa, non-reducing conditions

Activity

Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells.
The ED50 for this effect is 0.12-1.2 ng/mL.

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811.
The ED50 for this effect is 1.5-7.5 ng/mL.

Reviewed Applications

Read 3 reviews rated 4.3 using 7955-IL in the following applications:

Scientific Data Images for Recombinant Human IL-17A (Human Cell-expressed) Protein

Recombinant Human IL-17A (Human Cell-expressed) Protein Bioactivity

Recombinant Human IL-17A (Human Cell-expressed) Protein Bioactivity

Recombinant Human IL-17 (Catalog # 7955-IL) induces CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 0.12‑1.2 ng/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7955-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100-500 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7955-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17/IL-17A

Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL‑17A shares 60% amino acid sequence identity with mouse and rat IL‑17A (3, 4). IL‑17A is secreted by Th17 cells, gamma / delta  T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide‑linked homodimers as well as disulfide‑linked heterodimers with IL‑17F (5, 6). IL‑17A exerts its effects through the transmembrane IL‑17 RA in complex with IL‑17 RC or IL‑17 RD (7, 8). Both IL‑17 RA and IL‑17 RC are required for responsiveness to heterodimeric IL‑17A/F (7). IL‑17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL‑17A/F likewise induces neutrophil migration, but IL‑17F does not (11). IL‑17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF‑ alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL‑17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15). IL‑17A has been shown to exert either tumorigenic or anti‑tumor effects (16, 17).

References

  1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
  2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
  3. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  4. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
  5. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  6. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  7. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
  8. Rong, Z. et al. (2009) Cell Res. 19:208.
  9. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
  10. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
  11. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
  12. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
  13. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
  14. Hsu, H. et al. (2008) Nat. Immunol. 9:166.
  15. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
  16. Wang, L. et al. (2009) J. Exp. Med. 206:1457.
  17. Kryczek, I. et al. (2009) Blood 114:357.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Gene Symbol

IL17A

UniProt

Q16552

Additional IL-17/IL-17A Products

Product Documents for Recombinant Human IL-17A (Human Cell-expressed) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 7955-IL (with carrier)
Download SDS 7955-IL/CF (carrier free)

Product Specific Notices for Recombinant Human IL-17A (Human Cell-expressed) Protein

For research use only

Citations for Recombinant Human IL-17A (Human Cell-expressed) Protein

Customer Reviews for Recombinant Human IL-17A (Human Cell-expressed) Protein (3)

4.3 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
33%
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FAQs for Recombinant Human IL-17A (Human Cell-expressed) Protein

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  • Q: The specifications section on the website for Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, describes SDS-PAGE as a range of Molecular Weights. Why? 

    A: Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, has one N-linked glycosylation site  and seven O-linked glycosylation sites. The variable glycosylation results in multiple bands for this protein.

  • Q: What are the diferences between Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB) and Recombinant Human IL-17A (Human Cell-expressed) Protein (Catalog # 7955-IL)?

    A: There are multiple differences between Catalog # 317-ILB and 7955-IL.

    • 317-ILB is raised in E. coli, while 7955-IL is raised in Human Embryonic Kidney (HEK293) cells.
    • Human Cell-expressed 7955-IL has a lower endotoxin specification (<0.01 EU per 1 μg of the protein by the LAL method) compared to E. coli-expressed 317-ILB (<0.10 EU per 1 μg of the protein).
    • Human Cell-expressed 7955-IL exhibits greater activity in the CXCL1/GRO alpha secretion assay in HT‑29 human colon adenocarcinoma cells (0.12 - 1.2 ng/mL) compared to E. coli-expressed 317-ILB (0.4-4.0 ng/mL).

  • Q: The specifications section on the website for Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, describes SDS-PAGE as a range of Molecular Weights. Why? 

    A: Recombinant Human IL-17/IL-17A, Catalog # 7955-IL, has one N-linked glycosylation site  and seven O-linked glycosylation sites. The variable glycosylation results in multiple bands for this protein.

  • Q: What are the diferences between Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB) and Recombinant Human IL-17A (Human Cell-expressed) Protein (Catalog # 7955-IL)?

    A: There are multiple differences between Catalog # 317-ILB and 7955-IL.

    • 317-ILB is raised in E. coli, while 7955-IL is raised in Human Embryonic Kidney (HEK293) cells.
    • Human Cell-expressed 7955-IL has a lower endotoxin specification (<0.01 EU per 1 μg of the protein by the LAL method) compared to E. coli-expressed 317-ILB (<0.10 EU per 1 μg of the protein).
    • Human Cell-expressed 7955-IL exhibits greater activity in the CXCL1/GRO alpha secretion assay in HT‑29 human colon adenocarcinoma cells (0.12 - 1.2 ng/mL) compared to E. coli-expressed 317-ILB (0.4-4.0 ng/mL).

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