Recombinant Human IL-17F (NS0-expressed) Protein

Carrier Free

Catalog # Availability Size / Price Qty
1335-INS-025/CF
1335-INS-010/CF
1335-INS-100/CF

With Carrier

Catalog # Availability Size / Price Qty
1335-INS-025
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human IL-17F (NS0-expressed) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2-10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-17F protein
Arg31-Gln163, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Arg31
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
15.7 kDa (monomer)
SDS-PAGE
21 kDa, reducing conditions

Product Datasheets

1335-INS (with carrier)

1335-INS/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1335-INS

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1335-INS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: IL-17F

Interleukin-17F (also known as ML-1) is a of the IL-17 family of cytokines. Members of this family are involved in tissue homeostasis and demonstrate a structural motif termed a cysteine-knot that characterizes a large superfamily of growth factors. Although most cysteine-knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1 - 3). Human IL-17F is secreted as a glycosylated, 45 kDa, disulfide-linked homodimer. It is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence and a 133 aa mature region. As noted, the molecule contains a cysteine-knot that is formed from two sets of paired beta -strands that are connected by disulfide linkages (4 - 6). There is potentially one additional isoform that utilizes an alternate start site. This adds an additional 10 aa at the N-terminus (1, 7). 

When induced, IL-17F is preferentially translated as the 153 aa precursor (1). Initially, IL-17F was also reported as IL-24 (7). Since that time, the IL-24 designation has been reassigned to MDA-7, a member of the IL-10 family of molecules (note: IL-17E is synonymous with IL-25). Mature human IL-17F is 59%, 56%, 44% and 52% aa identical to mature rat, mouse, canine and bovine IL-17F, respectively. Within the IL-17 family, IL-17F is most closely related to IL-17/IL-17A at 50% aa identity (6). Human IL-17F is active on mouse and porcine cells (6). IL-17F is produced by activated CD4+ T cells, mast cells, basophils and monocytes (1, 4), and is inducible by IL-23 (4, 8). Targets for IL-17F include respiratory epithelium and endothelial cells which produce proinflammatory cytokines that promote neutrophil activation and influx (4, 5, 9, 10).

References
  1. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  2. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
  3. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
  4. Kawaguchi, M. et al. (2001) J. Immunol. 167:4430.
  5. Starnes, T. et al. (2001) J. Immunol. 167:4137.
  6. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  7. Hromes, R.A. and T.T. Starnes (2001) GenBank
    Accession # AAK83350.
  8. Langrish, C.L. et al. (2005) J. Exp. Med. 201:233.
  9. Mcallister, F. et al. (2005) J. Immunol. 175:404.
  10. Numasaki, M. et al. (2004) Immunol. Lett. 95:175.
Long Name
Interleukin 17F
Entrez Gene IDs
112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)
Alternate Names
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; interleukin 17F

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