IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.
Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera, CF
R&D Systems | Catalog # 118-AP
Key Product Details
- R&D Systems NS0-derived Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera (118-AP)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Structure / Form
Applications
Product Specifications
Source
| Human IL-18 R beta (Phe20 - Arg356) Accession # O95256 |
DIEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL‑18 R alpha /IL‑1 R5 Fc Chimera (Catalog # 816-LR).
The ED50 for this effect is 2‑6 µg/mL in the presence of 30 µg/mL of soluble IL-18 R alpha /Fc Chimera and 40 ng/mL of human IL-18.
Reviewed Applications
Read 3 reviews rated 4.7 using 118-AP in the following applications:
Formulation, Preparation, and Storage
118-AP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: IL-18 R beta/IL-1 R7
References
- Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
- Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional IL-18 R beta/IL-1 R7 Products
Product Documents for Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera, CF
For research use only
Citations for Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera, CF
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Customer Reviews for Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera, CF (3)
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Application: Cell migration/motilityVerified Customer | Posted 12/02/2020
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Application: Immunoassay StandardVerified Customer | Posted 10/10/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 10/07/2020
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Associated Pathways
Th1 Differentiation Pathway
