Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera, CF

R&D Systems | Catalog # 118-AP

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera (118-AP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

O95256

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all IL-18 R beta/IL-1 R7 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IL-18 R beta/IL-1 R7 protein
Human IL-18 R beta
(Phe20 - Arg356)
Accession # O95256		 DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe20

Predicted Molecular Mass

65.1 kDa (monomer)

SDS-PAGE

78-93 kDa, reducing conditions

Activity

Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL‑18 R alpha /IL‑1 R5 Fc Chimera (Catalog # 816-LR).
The ED50 for this effect is 2‑6 µg/mL in the presence of 30 µg/mL of soluble IL-18 R alpha /Fc Chimera and 40 ng/mL of human IL-18.

Reviewed Applications

Read 3 reviews rated 4.7 using 118-AP in the following applications:

Formulation, Preparation, and Storage

118-AP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18 R beta/IL-1 R7

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

References

  1. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  2. Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.

Long Name

Interleukin 18 Receptor beta

Alternate Names

AcPL, CD218b, IL-1 R7, IL-18Rb, IL18R beta, IL18RAP

Entrez Gene IDs

8807 (Human); 16174 (Mouse)

Gene Symbol

IL18RAP

UniProt

O95256

Additional IL-18 R beta/IL-1 R7 Products

Product Documents for Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera, CF

For research use only

Citations for Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera, CF

Customer Reviews for Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera, CF (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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    Application: Cell migration/motility
    Verified Customer | Posted 12/02/2020
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    Application: Immunoassay Standard
    Verified Customer | Posted 10/10/2020
  • Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera, CF
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/07/2020

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