Recombinant Human IL-19 (NS0-expressed) Protein

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is typically 0.1-0.6 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Leu25-Ala177
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu25
  • Predicted Molecular Mass
    18 kDa
  • SDS-PAGE
    20-30 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9286-IL
 
9286-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human IL-19 (Catalog # 9286-IL) induces proliferation of BaF3 mouse pro-B cellstransfected with
human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is
0.1-0.6 ng/mL.
Background: IL-19
Interleukin 19 (IL-19) is a member of the IL-10 family of class II alpha -helical cytokines that contains two groups, a viral homolog and cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R beta as a signal transducing receptor (IL-10, IL-22, and IL-26), and one which uses IL-20 R beta as a signal transducing receptor (IL-19, IL-20, and IL-24) (1). The 153 amino acid (aa) mature human IL-19 is secreted as a glycosylated 35-45 kDa monomer (2-4). It shares approximately 70% aa sequence identity with mouse and rat IL-19. Human IL-19 may utilize an alternate start site that adds 38 aa to the N-terminus. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (2). IL-19 is expressed on activated keratinocytes, monocytes, and B cells (3, 5, 6). It binds a receptor complex consisting of IL-20 R alpha and IL-20 R beta (3, 7, 8). This receptor complex is also shared by IL-20 and IL-24 (9, 10). It has been reported that IL-19 both will and will not induce IL-6 and TNF-alpha production by monocytes (2, 11). It does, however, drive T helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (2, 11, 12).
  • References:
    1. Commins, S. et al. (2008) J. Allergy Clin. Immunol. 121:1108.
    2. Liao, Y-C. et al. (2002) J. Immunol. 169:4288.
    3. Gallagher, G. et al. (2000) Genes Immun. 1:442.
    4. Pletnev, S. et al. (2003) Biochemistry 42:12617.
    5. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
    6. Wolk, K. et al. (2002) J. Immunol. 168:5397.
    7. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
    8. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517.
    9. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
    10. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
    11. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
    12. Liao, S-C. et al. (2004) J. Immunol. 173:6712.
  • Long Name:
    Interleukin 19
  • Entrez Gene IDs:
    29949 (Human); 329244 (Mouse)
  • Alternate Names:
    IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19
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