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Recombinant Human IL-20 Protein

Catalog #: 1102-IL
12 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
1102-IL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1102-IL-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (12)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-20 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is 0.05-0.3 ng/mL.
Source
E. coli-derived human IL-20 protein
Leu25-Glu176, with an N-terminal Met
Accession #
AAF36679
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
17.6 kDa

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1102-IL (with carrier)

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1102-IL/CF (carrier free)

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1102-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1102-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-20

Human Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences containing a signal sequence and amphipathic helices found in helical cytokines. Human IL-20 is synthesized as a 176 amino acid (aa) precursor with a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL-20 share 77% aa sequence identity in their mature segments. Human IL-20 is active on mouse cells. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first is composed of IL-20 R alpha andIL-20 R beta. The second is composed of IL-22 R and IL-20 R beta. Whereas the IL-22 R/IL-20 R beta complex is shared with IL-24/mda-7, the IL-20 R alpha /IL-20 R beta  complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1 - 6).

References
  1. Blumberg, H. et al. (2001) Cell 104:9.
  2. Liu, L. et al. (2003) Blood 102:3206. 
  3. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.
  4. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
  5. Dumoutier, L. (2001) J. Immunol. 167:3545. 
  6. Romer, J. (2003) J. Invest. Dermatol. 121:1306.
Long Name
Interleukin 20
Entrez Gene IDs
50604 (Human); 58181 (Mouse)
Alternate Names
Cytokine Zcyto10; IL10D; IL20; IL-20; IL-20ZCYTO10four alpha helix cytokine; interleukin 20; interleukin-20; MGC96907

Citations for Recombinant Human IL-20 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation
    Authors: JR Dayton, Y Yuan, LP Pacumio, BG Dorflinger, SC Yoo, MJ Olson, SI Hernández-, MM McMahon, L Cruz-Oreng
    Frontiers in Cellular Neuroscience, 2021;15(0):683687.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. TgROP18 targets IL20RB for host-defense-related-STAT3 activation during Toxoplasma gondii infection
    Authors: L Kong, D Jiang, C He, J Xia, H Wei, L Zhou, H Peng
    Parasit Vectors, 2020;13(1):400.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. TgROP18 targets IL20RB for host-defense-related-STAT3 activation during Toxoplasma gondii infection
    Authors: L Kong, D Jiang, C He, J Xia, H Wei, L Zhou, H Peng
    Parasit Vectors, 2020;13(1):400.
    Species: Cercopithecus aethiops
    Sample Types: Transduced Whole Cells
    Applications: Bioassay
  4. Interleukin (IL)-22 from IL-20 Subfamily of Cytokines Induces Colonic Epithelial Cell Proliferation Predominantly through ERK1/2 Pathway
    Authors: M Moniruzzam, R Wang, V Jeet, MA McGuckin, SZ Hasnain
    Int J Mol Sci, 2019;20(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. IL-20 Signaling in Activated Human Neutrophils Inhibits Neutrophil Migration and Function
    Authors: P Gough, S Ganesan, SK Datta
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Addiction to the IGF2-ID1-IGF2 circuit for maintenance of the breast cancer stem-like cells
    Oncogene, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. MMP8 Is Increased in Lesions and Blood of Acne Inversa Patients: A Potential Link to Skin Destruction and Metabolic Alterations
    Authors: Robert Sabat
    Mediators Inflamm., 2016;2016(0):4097574.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Induction of phenotype modifying cytokines by FERMT1 mutations.
    Authors: Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C
    Hum. Mutat., 2011;32(4):397-406.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
    Authors: Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R
    Eur. J. Immunol., 2009;39(12):3570-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression.
    Authors: Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K
    Eur. J. Immunol., 2009;39(10):2779-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Maturing dendritic cells are an important source of IL-29 and IL-20 that may cooperatively increase the innate immunity of keratinocytes.
    Authors: Wolk K, Witte K, Witte E, Proesch S, Schulze-Tanzil G, Nasilowska K, Thilo J, Asadullah K, Sterry W, Volk HD, Sabat R
    J. Leukoc. Biol., 2008;83(5):1181-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. The effects of IL-20 subfamily cytokines on reconstituted human epidermis suggest potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis.
    Authors: Sa SM, Valdez PA, Wu J
    J. Immunol., 2007;178(4):2229-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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By Bo Chen on 12/03/2018
Application: In vitro bioactivity in cell culture