Recombinant Human IL-20 R beta Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1788-IR-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human IL-20 R beta Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑19 (Catalog # 1035‑IL) is present at 0.1 μg/mL, the concentration of Recombinant Human IL‑20 R beta Fc Chimera that produces 50% of the optimal binding response is approximately 10‑50 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-20 R beta protein
Human IL-20 R beta
(Asp30-Ala230)
Accession # Q6UXL0
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asp30
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49.2 kDa (monomer)
SDS-PAGE
65 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1788-IR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-20 R beta

IL-20 receptor beta (IL-20 R beta ), also known as IL-20 R2, is a transmembrane glycoprotein in the class II cytokine receptor family. These receptors are characterized by tandem fibronectin type III domains in their extracellular region and the lack of a WSXWS motif (1). Class II cytokine receptors form heterodimeric signaling receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1). Human IL-20 R beta consists of a 204 aa extracellular domain (ECD) with two fibronectin type III domains, a 21 aa transmembrane segment, and a 57 aa cytoplasmic domain (2). Within the ECD, human IL-20 R beta shares 81% aa sequence identity with mouse IL-20 R beta. It shares 15%-28% aa sequence identity with the human class II cytokine receptors IFN‑ alpha  R1, IFN-alpha R2, IFN-gamma R1, IFN-gamma R2, IL-10 R alpha, IL-10 R beta, IL-20 R alpha, IL-22BP, IL-22 R alpha, IL-28 R, and tissue factor. Strong IL-20 R beta expression is normally restricted to skin and testis (3). IL-20 R beta is also expressed in psoriatic skin, rheumatoid arthritis synovial membranes, and hepatocytes of LPS-treated mice, and it contributes to the local inflammatory reaction (3-6). IL-20 R beta heterodimerizes with IL-20 R alpha to form the receptor complex that mediates IL-19, IL-20 and IL-24 signals (3, 7-10). It also heterodimerizes with IL-22 R to form the functional receptor complex for IL-20 and IL-24 (7-9). Binding of these IL-10 family class II cytokines to their receptors induces activation of the JAK-STAT signal transduction pathway.

References
  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. SwissProt # Q6UXL0.
  3. Blumberg, H. et al. (2001) Cell 104:9.
  4. Hsu, Y.-H. et al. (2006) Arthritis Rheum. 54:2722.
  5. Wegenka, U.M. et al. (2007) J. Hepatol. 46:257.
  6. Chan, J.R. et al. (2006) J. Exp. Med. 203:2577.
  7. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517.
  8. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  9. Wang, M., et al. (2002) J. Biol. Chem. 277:7341.
  10. Pletnev, S. et al. (2003) Biochemistry 42:12617.
Long Name
Interleukin 20 Receptor beta
Entrez Gene IDs
53833 (Human); 213208 (Mouse)
Alternate Names
FNDC6; DIRS1; FNDC6; IL-20 R beta; IL20R beta; IL-20R2; IL20RB interleukin 20 receptor beta; IL20RB; IL-20Rb; MGC34923

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Recombinant Human IL-20 R beta Fc Chimera Protein, CF
By Anonymous on 06/07/2018
Application: Binding assay/Protein-protein interaction