Recombinant Human IL-24 Protein

Formulations:
Catalog # Availability Size / Price Qty
1965-IL-025
Product Details
Citations (15)
FAQs
Reviews

Recombinant Human IL-24 Protein Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is 15-150 pg/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-24 protein
Met28-Leu206, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Gln52 predicted, no results obtained, sequencing might be blocked.
Predicted Molecular Mass
19.5 kDa
SDS-PAGE
26-35 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1965-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1965-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-24

Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids (aa) that contains a 48 aa signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35 - 40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL‑24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN‑ gamma, IL‑1 beta, IL‑12 and TNF-alpha. At high concentrations, it is a strong inducer of apoptosis in tumor cells, but not normal cells. IL‑24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL‑20 R alpha and IL-20 R beta, which is shared with IL-19 and IL‑20. The second complex is a combination of IL‑22 R and IL-20 R beta, which is shared with IL-20.

References
  1. Jiang, H. et al. (1995) Oncogene 11:2477.
  2. Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160.
  3. Wang, M. et al. (2002) J. Biol Chem. 227:7341.
  4. Chada, S. et al. (2004) Int. Immunopharmacol. 4:649.
  5. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  6. Chen, J. et al. (2003) Molec. Ther. 8:220.
Long Name
Interleukin 24
Entrez Gene IDs
11009 (Human); 93672 (Mouse); 170819 (Rat)
Alternate Names
C49A; FISP; IL10B; IL24; IL-24; interleukin 24; MDA-7; MDA7MOB5; melanocyte-associated Mda-7; melanoma differentiation association protein 7; Melanoma differentiation-associated gene 7 protein; MOB-5; ST16; ST16interleukin-24; suppression of tumorigenicity 16 (melanoma differentiation); Suppression of tumorigenicity 16 protein

Citations for Recombinant Human IL-24 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Integrated microRNA/mRNA expression profiling of the skin of psoriasis patients
    Authors: D Deli?, K Wolk, R Schmid, O Gabrielyan, D Christou, K Rieber, M Rolser, I Jakob, F Wiech, M Griesser, C Wohnhaas, G Kokolakis, E Witte-Händ, P Baum, R Sabat
    J. Dermatol. Sci., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Iron-induced calcification in human aortic vascular smooth muscle cells through interleukin-24 (IL-24), with/without TNF-alpha
    Authors: S Kawada, Y Nagasawa, M Kawabe, H Ohyama, A Kida, N Kato-Kogoe, M Nanami, Y Hasuike, T Kuragano, H Kishimoto, K Nakasho, T Nakanishi
    Sci Rep, 2018;8(1):658.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Modulation of Th1/Tc1 and Th17/Tc17 responses in pulmonary tuberculosis by IL-20 subfamily of cytokines
    Authors: NP Kumar, K Moideen, VV Banurekha, D Nair, S Babu
    Cytokine, 2018;108(0):190-196.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Microarray Analysis Reveals Increased Expression of Matrix Metalloproteases and Cytokines of Interleukin-20 Subfamily in the Kidneys of Neonate Rats Underwent Unilateral Ureteral Obstruction: A Potential Role of IL-24 in the Regulation of Inflammation and Tissue Remodeling
    Authors: D Pap, E Sziksz, Z Kiss, R Rokonay, A Veres-Szék, R Lippai, IM Takács, É Kis, A Fekete, G Reusz, A Vannay, AJ Szabó
    Kidney Blood Press. Res, 2017;42(1):16-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas
    Authors: BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. MMP8 Is Increased in Lesions and Blood of Acne Inversa Patients: A Potential Link to Skin Destruction and Metabolic Alterations
    Authors: Robert Sabat
    Mediators Inflamm., 2016;2016(0):4097574.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Tumor necrosis factor receptor signaling in keratinocytes triggers interleukin-24-dependent psoriasis-like skin inflammation in mice.
    Authors: Kumari S, Bonnet M, Ulvmar M, Wolk K, Karagianni N, Witte E, Uthoff-Hachenberg C, Renauld J, Kollias G, Toftgard R, Sabat R, Pasparakis M, Haase I
    Immunity, 2013;39(5):899-911.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  8. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Induction of phenotype modifying cytokines by FERMT1 mutations.
    Authors: Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C
    Hum. Mutat., 2011;32(4):397-406.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells.
    Authors: Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R, Dalloul A
    Blood, 2010;115(9):1718-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression.
    Authors: Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K
    Eur. J. Immunol., 2009;39(10):2779-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
    Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
    Int. J. Cancer, 2009;125(2):367-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. The effects of IL-20 subfamily cytokines on reconstituted human epidermis suggest potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis.
    Authors: Sa SM, Valdez PA, Wu J
    J. Immunol., 2007;178(4):2229-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. IL-24 modulates IFN-gamma expression in patients with tuberculosis.
    Authors: Wu B, Huang C, Kato-Maeda M, Hopewell PC, Daley CL, Krensky AM, Clayberger C
    Immunol. Lett., 2007;117(1):57-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Systemic cancer gene therapy using adeno-associated virus type 1 vector expressing MDA-7/IL24.
    Authors: Tahara I, Miyake K, Hanawa H, Kurai T, Hirai Y, Ishizaki M, Uchida E, Tajiri T, Shimada T
    Mol. Ther., 2007;15(10):1805-11.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

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