Recombinant Human IL-29/IFN-lambda 1 Protein, CF

R&D Systems | Catalog # 11240-IL

E. coli-expressed
R&D Systems
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human IL-29/IFN-lambda 1 Protein (11240-IL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q8IU54.1

Applications

Bioactivity
View all IL-29/IFN-lambda 1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-29/IFN-lambda 1 protein
Pro23-Thr200

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro23

Predicted Molecular Mass

20 kDa

SDS-PAGE

18-22 kDa, under reducing conditions.

Activity

Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
The ED50 for this effect is 0.500-6.00 ng/mL.

Scientific Data Images for Recombinant Human IL-29/IFN-lambda 1 Protein, CF

Recombinant Human IL-29/IFN-lambda 1 Protein Bioactivity.

Recombinant Human IL‑29/IFN‑lambda 1 Protein (Catalog # 11240-IL) demonstrates anti-viral activity in HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.500-6.00 ng/mL.

Formulation, Preparation, and Storage

11240-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-29/IFN-lambda 1

IL-29, also known interferon-lambda 1 (IFN lambda 1), along with IL-28A (IFN lambda 2), IL-28B (IFN lambda 3) and IFN lambda 4, collectively comprise the type III subset of IFNs. IFN lambda s are class II cytokine receptor ligands distantly related to both members of the IL-10 family and to the type I IFN family (11-19% amino acid (aa) sequence identity). IL-29 is expressed only in humans and shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively. IFN lambda signaling was initially thought to be restricted to epithelial cells, but, recently, additional cell types have been shown to also respond. IFN lambda s have been shown to be induced by a variety of stimuli from influenza A and herpes viruses to lipopolysaccharides and double-stranded RNA. IL-29 signals through a unique receptor complex composed of IFN lambda R1 (IL-28RA) and the shared IL-10R2 chain, which is also a part of the receptor complexes for IL-10, IL-22, and IL-26. In humans, IL-29 is the most potent IFN lambda molecule, the most abundant IFN lambda molecule in serum, and the only IFN lambda molecule to display N-linked glycosylation. IL-29 is being investigated for its role in immunity, both innate and adaptive, as well as in autoimmune disorders. Additionally, the therapeutic potential of IL-29 in inflammatory diseases and its cancer fighting properties are being actively studied.

References

  1. Vilcek, J. (2003) Nature Immunol. 4:8.
  2. Sheppard, P. et al. (2003) Nature Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.
  4. Gad, H.H. et al. (2009) J. Biol. Chem. (2009) 284:20869.
  5. Miknis, Z.J. et al. (2010) J. Mol. Biol. 404:650.
  6. Donnelly, R.P. et al. (2010) J. Interferon. Cytokine Res. 30:555.
  7. Lazear, H.M. et al. (2015) Immunity. 43:15.
  8. Ye, L. et al. (2019) Nat. Rev. Immunol. 19:614.
  9. Goel, R.R. et al. (2021) Nat. Rev. Rheumatol. 17:349.
  10. Kelm, N.E. et al. (2016) Crit. Rev. Oncol. Hematol. 106:91.
  11. Wang, J.M. et al. (2019) J. Cell Mol. Med. 23:7926.

Long Name

Interleukin 29

Alternate Names

IFN-lambda 1, IL29

Entrez Gene IDs

282618 (Human)

Gene Symbol

IFNL1

UniProt

Q8IU54.1

Additional IL-29/IFN-lambda 1 Products

Product Documents for Recombinant Human IL-29/IFN-lambda 1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-29/IFN-lambda 1 Protein, CF

For research use only

Related Research Areas

Interferons

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