hh Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein, CF 6834-ILB-025/CF: R&D Systems

Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein, CF

(2 citations)   
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 0.8-4.8 ng/mL.
  • Source
    E. coli-derived Arg5-Glu157
  • Accession #
  • N-terminal Sequence
    Analysis
    Arg5
  • Predicted Molecular Mass
    17 kDa
  • SDS-PAGE
    17 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6834-ILB/CF
 
6834-ILB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human IL-36 beta/IL-1F8 (Catalog #
6834-ILB/CF) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 0.8-4.8 ng/mL.
Background: IL-36 beta/IL-1F8
Interleukin-36 beta (IL-36 beta, previously known as IL-1F8, FIL-1 eta (eta) and IL-1H2, is a member of the IL-1 family of proteins that also includes IL-1 beta, IL-1 alpha, IL-1ra,
IL-18, IL-36Ra/IL-1F5, IL-36 alpha /IL-1F6, IL-37/IL-1F7, IL-36 gamma /IL-1F9, and IL-1F10 (1, 2). IL-1 family cytokines are characterized by a 12  beta -stranded beta -trefoil configuration and share up to 50% amino acid (aa) sequence identity. The 157 aa human IL-36 beta does not have a canonical signal peptide or prosegment and is expressed as two isoforms that differ in their C-terminal 70 aa. IL-36 beta 1 lacks four of the conserved beta -strands common to the IL-1 family (3). Human IL-36 beta 2 shares 62%, 67%, 63% and 59% aa identity with the most similar isoforms of mouse, canine, bovine and equine IL-36 beta, respectively (4). IL-36 beta is expressed by keratinocytes, naïve CD4+ T cells, neurons, and glia (5-7). It is up-regulated in keratinocytes and synovial fibroblasts by inflammatory stimulation and in psoriatic lesions (5, 8, 9). IL-36 beta promotes inflammatory responses by enhancing the activation and Th1 polarization of dendritic cells and T cells (7, 10, 11). It also enhances the production of multiple pro-inflammatory cytokines, chemokines, and anti-bacterial defensin peptides by keratinocytes, synovial fibroblasts, and articular chondrocytes (5, 8-10). IL-36 proteins exert their bioactivity through a receptor complex that contains IL-1 Rrp2 and IL-1 RAcP, and this is antagonized by IL-36Ra which also binds IL-1 Rrp2 (11, 12). The potency of IL-36 beta is increased by cleavage of its first four N-terminal amino acids (13).
  • References:
    1. Garlanda, C. et al. (2013) Immunity 39:1003.
    2. Gresnigt, M.S. and F.L. van de Veerdonk (2013) Semin. Immunol. 25:458.
    3. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
    4. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
    5. Johnston, A. et al. (2011) J. Immunol. 186:2613.
    6. Wang, P. et al. (2005) Cytokine 29:245.
    7. Vigne, S. et al. (2012) Blood 120:3478.
    8. Magne, D. et al. (2005) Arthritis Res. Ther. 8:R80.
    9. Carrier, Y. et al. (2011) J. Invest. Dermatol. 131:2428.
    10. Foster, A.M. et al. (2014) J. Immunol. 192:6053.
    11. Vigne, S. et al. (2011) Blood 118:5813.
    12. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
    13. Towne, J.E. et al. (2011) J. Biol. Chem. 286:42594.
  • Long Name:
    Interleukin 36 beta/Interleukin 1 Family 8
  • Entrez Gene IDs:
    27177 (Human); 69677 (Mouse)
  • Alternate Names:
    family of interleukin 1-eta; FIL1 eta; FIL1; FIL1-(ETA); FIL1H; FILI-(ETA); IL-1 eta; IL1-ETA; IL1F8 (Canonical product IL-1F8a); IL-1F8 (FIL1-eta); IL1F8; IL-1F8; IL1H2; IL-1H2; IL36 beta; IL-36 beta; IL36B; interleukin 1 family, member 8 (eta); interleukin 1, eta; Interleukin 36, Beta; Interleukin-1 eta; interleukin-1 family member 8; Interleukin-1 homolog 2; Interleukin-1 Superfamily e; Interleukin-36 Beta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Structural and functional attributes of the Interleukin-36 receptor
    Authors: Guanghui Yi
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Signal Transduction and Intracellular Trafficking by the Interleukin 36 Receptor.
    Authors: Saha S, Singh D, Raymond E, Ganesan R, Caviness G, Grimaldi C, Woska J, Mennerich D, Brown S, Mbow M, Kao C
    J Biol Chem, 2015;290(39):23997-4006.

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