Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
6834-ILB-025/CF
Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein, CF Bioactivity
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Product Details
Citations (2)
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Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein, CF Summary

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 0.8-4.8 ng/mL.
Source
E. coli-derived human IL-36 beta/IL-1F8 protein
Arg5-Glu157
Accession #
N-terminal Sequence
Analysis
Arg5
Predicted Molecular Mass
17 kDa
SDS-PAGE
17 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6834-ILB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6834-ILB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein, CF Bioactivity View Larger

Recombinant Human IL-36 beta/IL-1F8 (Catalog #
6834-ILB/CF) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 0.8-4.8 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: IL-36 beta/IL-1F8

Interleukin-36 beta (IL-36 beta, previously known as IL-1F8, FIL-1 eta (eta) and IL-1H2, is a member of the IL-1 family of proteins that also includes IL-1 beta, IL-1 alpha, IL-1ra,
IL-18, IL-36Ra/IL-1F5, IL-36 alpha /IL-1F6, IL-37/IL-1F7, IL-36 gamma /IL-1F9, and IL-1F10 (1, 2). IL-1 family cytokines are characterized by a 12  beta -stranded beta -trefoil configuration and share up to 50% amino acid (aa) sequence identity. The 157 aa human IL-36 beta does not have a canonical signal peptide or prosegment and is expressed as two isoforms that differ in their C-terminal 70 aa. IL-36 beta 1 lacks four of the conserved beta -strands common to the IL-1 family (3). Human IL-36 beta 2 shares 62%, 67%, 63% and 59% aa identity with the most similar isoforms of mouse, canine, bovine and equine IL-36 beta, respectively (4). IL-36 beta is expressed by keratinocytes, naïve CD4+ T cells, neurons, and glia (5-7). It is up-regulated in keratinocytes and synovial fibroblasts by inflammatory stimulation and in psoriatic lesions (5, 8, 9). IL-36 beta promotes inflammatory responses by enhancing the activation and Th1 polarization of dendritic cells and T cells (7, 10, 11). It also enhances the production of multiple pro-inflammatory cytokines, chemokines, and anti-bacterial defensin peptides by keratinocytes, synovial fibroblasts, and articular chondrocytes (5, 8-10). IL-36 proteins exert their bioactivity through a receptor complex that contains IL-1 Rrp2 and IL-1 RAcP, and this is antagonized by IL-36Ra which also binds IL-1 Rrp2 (11, 12). The potency of IL-36 beta is increased by cleavage of its first four N-terminal amino acids (13).

References
  1. Garlanda, C. et al. (2013) Immunity 39:1003.
  2. Gresnigt, M.S. and F.L. van de Veerdonk (2013) Semin. Immunol. 25:458.
  3. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  4. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  5. Johnston, A. et al. (2011) J. Immunol. 186:2613.
  6. Wang, P. et al. (2005) Cytokine 29:245.
  7. Vigne, S. et al. (2012) Blood 120:3478.
  8. Magne, D. et al. (2005) Arthritis Res. Ther. 8:R80.
  9. Carrier, Y. et al. (2011) J. Invest. Dermatol. 131:2428.
  10. Foster, A.M. et al. (2014) J. Immunol. 192:6053.
  11. Vigne, S. et al. (2011) Blood 118:5813.
  12. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  13. Towne, J.E. et al. (2011) J. Biol. Chem. 286:42594.
Long Name
Interleukin 36 beta/Interleukin 1 Family 8
Entrez Gene IDs
27177 (Human); 69677 (Mouse)
Alternate Names
family of interleukin 1-eta; FIL1 eta; FIL1; FIL1-(ETA); FIL1H; FILI-(ETA); IL-1 eta; IL1-ETA; IL1F8 (Canonical product IL-1F8a); IL-1F8 (FIL1-eta); IL1F8; IL-1F8; IL1H2; IL-1H2; IL36 beta; IL-36 beta; IL36B; interleukin 1 family, member 8 (eta); interleukin 1, eta; Interleukin 36, Beta; Interleukin-1 eta; interleukin-1 family member 8; Interleukin-1 homolog 2; Interleukin-1 Superfamily e; Interleukin-36 Beta

Citations for Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Structural and functional attributes of the Interleukin-36 receptor
    Authors: Guanghui Yi
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Signal Transduction and Intracellular Trafficking by the Interleukin 36 Receptor.
    Authors: Saha S, Singh D, Raymond E, Ganesan R, Caviness G, Grimaldi C, Woska J, Mennerich D, Brown S, Mbow M, Kao C
    J Biol Chem, 2015;290(39):23997-4006.

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