Recombinant Human IL-39 Fc Chimera Protein, CF Summary
Accession # Q14213
Accession # Q9NPF7
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
Recombinant Human IL-39 Fc Chimera (Catalog # 9990-IL) induces IFN-gamma production in mouse splenocytes. The ED50for this effect is 50-250 ng/mL.
2 μg/lane of Recombinant Human IL-39 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-77 kDa and 140-160 kDa, respectively.
Background: IL-39 (IL-23p19/EBI3)
Interleukin 39 (IL-39) is a member of the IL-12 family of heterodimeric cytokines. IL-12 cytokines are composed of an alpha and beta subunit which, for IL-39 are the IL-23 p19 subunit and the EBI3 subunit, respectively (1-3). The IL-23 p19 subunit of IL-39 is synthesized as a 189 amino acid (aa) precursor protein with a 19 aa signal sequence and a 170 aa mature region. The EBI3 subunit of IL-39 is synthesized as a 229 aa precursor protein that contains a 20 aa signal sequence and a 209 aa mature region. Human and mouse IL-39 share 73% and 62% sequence homology in their IL-23 p19 and EBI3 subunits, respectively. Heterodimer (IL-39) is secreted by LPS-stimulated B cells and GL7(+) activated B cells of lupus-like mice (1, 4). IL-39 signals through IL-23 R/gp130 receptors and mediates inflammatory responses through activation of STAT1 and STAT3 in lupus-like mice (1, 2). IL‐39 induces and/or expands neutrophils in lupus‐prone mice, and IL‐39‐induced neutrophils increase the secretion of B cell activation factor (BAFF) (3). Thus, IL-39 is an important pro-inflammatory cytokine and play important roles in the pathophysiology of autoimmune diseases, including Systemic Lupus Erythematosus (SLE) (2, 4).
- Wang, X. et al. (2016) Eur J Immunol 46:1343.
- Hasegawa, H. et al. (2016) Front Immunol 7:479.
- Wang, X. et al. (2016) Clin Exp Immunol 186:144.
- Wang, X. et al. (2018) Mol Med Rep 17:1660.
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