Recombinant Human IL-39 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9990-IL-050
Recombinant Human IL-39 Fc Chimera Protein, CF Bioactivity
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Recombinant Human IL-39 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IFN-gamma production by mouse splenocytes. The ED50 for this effect is 50-250 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human IL-39 (IL-23p19/EBI3) protein
Human EBI3
(Arg21-Lys229, Gln95Cys)
Accession # Q14213
GGGSGGGSGGGS
Human IL-23p19
(Arg20-Pro189)
Accession # Q9NPF7
IEGRMDHuman IgG1
(Pro100-Lys330)
Accession #
N-terminal Sequence
Analysis
Arg21 (EBI3)
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
69 kDa
SDS-PAGE
70-77 kDa, reducing conditions

Product Datasheets

9990-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human IL-39 Fc Chimera Protein, CF Bioactivity View Larger

Recombinant Human IL-39 Fc Chimera (Catalog # 9990-IL) induces IFN-gamma production in mousesplenocytes. The ED50 for this effect is 50-250 ng/mL.

SDS-PAGE Recombinant Human IL-39 Fc Chimera Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human IL-39 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 70-77 kDa and 140-160 kDa, respectively.

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Background: IL-39 (IL-23p19/EBI3)

Interleukin 39 (IL-39) is a member of the IL-12 family of heterodimeric cytokines. IL-12 cytokines are composed of an alpha and beta subunit which, for IL-39 are the IL-23 p19 subunit and the EBI3 subunit, respectively (1-3). The IL-23 p19 subunit of IL-39 is synthesized as a 189 amino acid (aa) precursor protein with a 19 aa signal sequence and a 170 aa mature region. The EBI3 subunit of IL-39 is synthesized as a 229 aa precursor protein that contains a 20 aa signal sequence and a 209 aa mature region. Human and mouse IL-39 share 73% and 62% sequence homology in their IL-23 p19 and EBI3 subunits, respectively. Heterodimer (IL-39) is secreted by LPS-stimulated B cells and GL7(+) activated B cells of lupus-like mice (1, 4). IL-39 signals through IL-23 R/gp130 receptors and mediates inflammatory responses through activation of STAT1 and STAT3 in lupus-like mice (1, 2). IL‐39 induces and/or expands neutrophils in lupus‐prone mice, and IL‐39‐induced neutrophils increase the secretion of B cell activation factor (BAFF) (3). Thus, IL-39 is an important pro-inflammatory cytokine and play important roles in the pathophysiology of autoimmune diseases, including Systemic Lupus Erythematosus (SLE) (2, 4).

References
  1. Wang, X. et al. (2016) Eur J Immunol 46:1343.
  2. Hasegawa, H. et al. (2016) Front Immunol 7:479.
  3. Wang, X. et al. (2016) Clin Exp Immunol 186:144.
  4. Wang, X. et al. (2018) Mol Med Rep 17:1660.
Long Name
Interleukin 39 (IL-23p19/EBI3 Heterodimer)
Entrez Gene IDs
51561 (Human); 83430 (Mouse)
Alternate Names
IL-23 p19/EBI3; IL-23p19/EBI3; IL-39 (IL-23p19/EBI3)

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Primary Antibodies

Human IgG Fc Antibody

WB,Flow,CyTOF-ready

Human IgG1 Antibody

Recombinant Proteins

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