Recombinant Human ILDR1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10152-D1-050
Recombinant Human ILDR1 Fc Chimera Protein, CF SDS-PAGE
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Recombinant Human ILDR1 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human ILDR1 Fc Chimera is immobilized at 2.5 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human ILDR2 Fc Chimera that produces 50% of the optimal binding response is 2-12 μg/mL
Source
Human embryonic kidney cell, HEK293-derived human ILDR1 protein
Human ILDR1
(Leu24-His167)
Accession # Q86SU0
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Leu24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43 kDa
SDS-PAGE
40-49 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10152-D1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human ILDR1 Fc Chimera(Catalog # 10152-D1) was resolved with SDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by Coomassie®Blue staining, showing bands at 40-49 kDa and 80-100 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ILDR1

Immunoglobulin-like domain containing receptor 1, or ILDR1, is a single pass Type I membrane protein that belongs to the immunoglobulin superfamily. Human ILDR1 contains a signal peptide (aa 1-23), an Ig-like V-type extracellular domain (aa 24-167), a helical transmembrane domain amino acids (aa168-188), and a cytoplasmic domain (aa 189-546) that contains a cysteine rich region and an arginine rich region (1). The extracellular domain of human ILDR1 shares a 96.5% homology with mouse and rat respectively. ILDR1 is mainly expressed in the prostate, with lower levels also found in testis, pancreas, heart and liver (1). ILDR1 has also been found expressed by intestinal cholecystokinin (CCK)-producing cells (2). ILDR1 has three splice variants. Two of those are expressed on the cell surface, while one is expressed in the cytoplasm, except when co-expressed with one of the other two splice variants. The cytoplasmic variant is the shortest transcript and is not found in healthy tissue, but only in lymphoma samples (1). It is suggested that ILDR1 plays a role in regulating intestinal hormone levels by stimulating release of CCK in response to fatty acids (2). Loss of function of ILDR1 is associated with Autosomal-Recessive Hearing Impairment (3). Our data shows that ILDR1 binds to human immunoglobulin-like domain containing receptor 2 (ILDR2).

References
  1. Hauge, H. et al. (2004) Biochem. Biophys. Res. Commun. 323:970.
  2. Chandra, R. et al. (2013) J. Clin. Invest. 123:3343.
  3. Borck, G. et al. (2011) Am. J. Hum. Genet. 88:127.
Long Name
Immunoglobulin Like Domain Containing Receptor 1
Entrez Gene IDs
286676 (Human); 106347 (Mouse); 303914 (Rat)
Alternate Names
Angulin-2; autosomal recessive 42; DFNB42; ILDR1; ILDR1alpha; ILDR1alpha'; ILDR1beta; immunoglobulin-like domain containing receptor 1; immunoglobulin-like domain-containing receptor 1 alpha; immunoglobulin-like domain-containing receptor 1 alpha'; immunoglobulin-like domain-containing receptor 1 beta

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