Recombinant Human Insulin R/CD220 (aa 28-944) Protein

Carrier Free

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R&D Systems Recombinant Proteins and Enzymes
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Citations (7)

Recombinant Human Insulin R/CD220 (aa 28-944) Protein Summary

Product Specifications

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When 15 ng/mL of biotinylated recombinant human Insulin is added to serially diluted Recombinant Human Insulin R/CD220, the concentration of Recombinant Human Insulin R/CD220 that produces 50% of the optimal binding response is 0.03‑0.15 μg/mL.
Mouse myeloma cell line, NS0-derived human Insulin R/CD220 protein
His28-Arg750 ( alpha subunit) & Ser751-Lys944 with a C-terminal 10-His tag ( beta subunit)
Accession #
N-terminal Sequence
His28 ( alpha subunit) & Ser751 ( beta subunit)
Structure / Form
Tetramer; disulfide-linked homodimer of disulfide-linked heterodimers ( alpha & beta )
Predicted Molecular Mass
82.9 kDa ( alpha subunit), 22.9 kDa ( beta subunit)
122-135 kDa and 33-43 kDa, reducing conditions

Product Datasheets

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1544-IR (with carrier)

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1544-IR/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Insulin R/CD220

The Insulin Receptor (gene name INSR, designated CD220) is a type I transmembrane glycoprotein in the Insulin/IGF Receptor family of receptor tyrosine kinases that share structural similarity and overlapping intracellular signaling events (1-3). The 1370 amino acid (aa) human Insulin R preproprotein (A isoform) is processed by proteolysis to remove the signal peptide and produce an extracellular alpha portion (aa 28-750), and an extracellular/transmembrane/cytoplasmic beta subunit (aa 751-1370) (4). The extracellular domain (ECD) contains two homologous globular domains separated by a cysteine-rich domain and followed by three fibronectin type III domains. The intracellular region contains insulin-receptor substrate (IRS) docking sites, the kinase domain, and a phosphotyrosine-containing linker region. The human Insulin R ECD shares 96% aa sequence identity with mouse, rat, equine and canine Insulin R. As a result of alternative splicing, two INSR isoforms that differ by the absence (IR-A) or presence (IR-B) of a 12 aa residue sequence in the carboxyl terminus of the alpha subunit exist (4). IR-A expression is highest in fetal tissues and cancer cells, while IR-B is concentrated in adult differentiated cells (2-5). IR-A and IR-B may homodimerize, or heterodimerize with the IGF-I receptor (1, 3, 4). All receptor combinations bind insulin, IGF-I or IGF-II, but with differing affinities; for example, IR-A has considerably higher affinity for IGF-II as compared to IR-B (2-5). This system allows fine tuning of signaling pathways according to the concentrations of insulin, IGF-I and IGF-II, and expression of receptor subunits on the cell surface (2, 3). Insulin R signaling regulates glucose uptake and metabolism, but also contributes to cell growth, differentiation and apoptosis (2, 3, 5, 6). Mutations in the Insulin R gene have been linked severe insulin resistance (type A and Rabson-Mendenhall syndrome) that may include type II diabetes mellitus and, rarely, leprechaunism (Donohue syndrome) that also includes growth delays and endocrine system abnormalities (1, 7). The R&D Systems human Insulin R consists of the entire ECD of the IR-A isoform.

  1. Nakae, J. et al. (2001) Endoc. Rev. 22:818.
  2. Sciacca, L. et al. (2003) Endocrinology 144:2650.
  3. Belfiore, A. et al. (2009) Endocrine Rev. 30:586.
  4. Lawrence, M.C. et al. (2007) Curr. Opin. Struct. Biol. 17:699.
  5. Sacco, A. et al. (2009) Endocrinology 150:3594.
  6. Kitamura, T. et al. (2004) J. Clin. Invest. 113:209.
  7. Musso, C. et al. (2004) Medicine (Baltimore) 83:209.
Long Name
Insulin Receptor
Entrez Gene IDs
3643 (Human); 16337 (Mouse)
Alternate Names
CD 220; CD220 antigen; CD220; EC 2.7.10; EC; HHF5; INSR; Insulin R; insulin receptor; InsulinR; IR

Citations for Recombinant Human Insulin R/CD220 (aa 28-944) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. An aptamer agonist of the insulin receptor acts as a positive or negative allosteric modulator, depending on its concentration
    Authors: NO Yunn, J Lee, HS Lee, EJ Oh, M Park, S Park, SY Jin, E Shin, JWY Lee, Y Kim, SS Bae, SH Ryu
    Experimental & Molecular Medicine, 2022;54(4):531-541.
    Species: N/A
    Sample Types: Aptamer
    Applications: Bioassay
  2. A hotspot for enhancing insulin receptor activation revealed by a conformation-specific allosteric aptamer
    Authors: NO Yunn, M Park, S Park, J Lee, J Noh, E Shin, SH Ryu
    Nucleic Acids Research, 2021;49(2):700-712.
    Species: Human
    Sample Types: DNA
    Applications: Bioassay
  3. Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease
    Authors: LL Lee, AY Khakoo, V Chintalgat
    FEBS Open Bio, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Structure of the Insulin Receptor-Insulin Complex by Single Particle CryoEM analysis
    Authors: G Scapin, VP Dandey, Z Zhang, W Prosise, A Hruza, T Kelly, T Mayhood, C Strickland, CS Potter, B Carragher
    Nature, 2018;0(0):.
    Applications: Bioassay
  5. A Small Insulinomimetic Molecule Also Improves Insulin Sensitivity in Diabetic Mice
    Authors: S Mukherjee, M Chattopadh, S Bhattachar, S Dasgupta, S Hussain, SK Bharadwaj, D Talukdar, A Usmani, BS Pradhan, SS Majumdar, P Chattopadh, S Mukhopadhy, TK Maity, MK Chaudhuri, S Bhattachar
    PLoS ONE, 2017;12(1):e0169809.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  6. Inhibition of insulin receptor function by a human, allosteric monoclonal antibody: a potential new approach for the treatment of hyperinsulinemic hypoglycemia.
    Authors: Corbin, John A, Bhaskar, Vinay, Goldfine, Ira D, Issafras, Hassan, Bedinger, Daniel H, Lau, Angela, Michelson, Kristen, Gross, Lisa M, Maddux, Betty A, Kuan, Hua F, Tran, Catarina, Lao, Llewelyn, Handa, Masahisa, Watson, Susan R, Narasimha, Ajay J, Zhu, Shirley, Levy, Raphael, Webster, Lynn, Wijesuriya, Sujeewa, Liu, Naichi, Wu, Xiaorong, Chemla-Vogel, David, Lee, Steve R, Wong, Steve, Wilcock, Diane, Rubin, Paul, White, Mark L
    MAbs, 2014;6(1):262-72.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Bioassay
  7. A novel ultra-sensitive enzyme immunoassay for soluble human insulin receptor ectodomain and its measurement in urine from healthy subjects and patients with diabetes mellitus.
    Authors: Umehara A, Nishioka M, Obata T
    Clin. Biochem., 2009;42(13):1468-75.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)


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