The Insulin Receptor (gene name INSR, designated CD220) is a type I transmembrane glycoprotein in the Insulin/IGF Receptor family of receptor tyrosine kinases that share structural similarity and overlapping intracellular signaling events (1-3). The 1370 amino acid (aa) human Insulin R preproprotein (A isoform) is processed by proteolysis to remove the signal peptide and produce an extracellular alpha portion (aa 28-750), and an extracellular/transmembrane/cytoplasmic beta subunit (aa 751-1370) (4). The extracellular domain (ECD) contains two homologous globular domains separated by a cysteine-rich domain and followed by three fibronectin type III domains. The intracellular region contains insulin-receptor substrate (IRS) docking sites, the kinase domain, and a phosphotyrosine-containing linker region. The human Insulin R ECD shares 96% aa sequence identity with mouse, rat, equine and canine Insulin R. As a result of alternative splicing, two INSR isoforms that differ by the absence (IR-A) or presence (IR-B) of a 12 aa residue sequence in the carboxyl terminus of the alpha subunit exist (4). IR-A expression is highest in fetal tissues and cancer cells, while IR-B is concentrated in adult differentiated cells (2-5). IR-A and IR-B may homodimerize, or heterodimerize with the IGF-I receptor (1, 3, 4). All receptor combinations bind insulin, IGF-I or IGF-II, but with differing affinities; for example, IR-A has considerably higher affinity for IGF-II as compared to IR-B (2-5). This system allows fine tuning of signaling pathways according to the concentrations of insulin, IGF-I and IGF-II, and expression of receptor subunits on the cell surface (2, 3). Insulin R signaling regulates glucose uptake and metabolism, but also contributes to cell growth, differentiation and apoptosis (2, 3, 5, 6). Mutations in the Insulin R gene have been linked severe insulin resistance (type A and Rabson-Mendenhall syndrome) that may include type II diabetes mellitus and, rarely, leprechaunism (Donohue syndrome) that also includes growth delays and endocrine system abnormalities (1, 7). The R&D Systems human Insulin R consists of the entire ECD of the IR-A isoform.
Best Seller
Recombinant Human Insulin R/CD220 (aa 28-944) Protein
R&D Systems | Catalog # 1544-IR
Loading...
Key Product Details
- R&D Systems NS0-derived Recombinant Human Insulin R/CD220 (aa 28-944) Protein (1544-IR)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Tetramer; disulfide-linked homodimer of disulfide-linked heterodimers ( alpha & beta )
Applications
Binding Activity
Loading...
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Insulin R/CD220 protein
His28-Arg750 ( alpha subunit) & Ser751-Lys944 with a C-terminal 10-His tag ( beta subunit)
His28-Arg750 ( alpha subunit) & Ser751-Lys944 with a C-terminal 10-His tag ( beta subunit)
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His28 ( alpha subunit) & Ser751 ( beta subunit)
Predicted Molecular Mass
82.9 kDa ( alpha subunit), 22.9 kDa ( beta subunit)
SDS-PAGE
122-135 kDa and 33-43 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When 15 ng/mL of biotinylated recombinant human Insulin is added to serially diluted Recombinant Human Insulin R/CD220, the concentration of Recombinant Human Insulin R/CD220 that produces 50% of the optimal binding response is 0.03‑0.15 μg/mL.
When 15 ng/mL of biotinylated recombinant human Insulin is added to serially diluted Recombinant Human Insulin R/CD220, the concentration of Recombinant Human Insulin R/CD220 that produces 50% of the optimal binding response is 0.03‑0.15 μg/mL.
Reviewed Applications
Read 2 reviews rated 3 using 1544-IR in the following applications:
Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1544-IR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1544-IR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: Insulin R/CD220
References
- Nakae, J. et al. (2001) Endoc. Rev. 22:818.
- Sciacca, L. et al. (2003) Endocrinology 144:2650.
- Belfiore, A. et al. (2009) Endocrine Rev. 30:586.
- Lawrence, M.C. et al. (2007) Curr. Opin. Struct. Biol. 17:699.
- Sacco, A. et al. (2009) Endocrinology 150:3594.
- Kitamura, T. et al. (2004) J. Clin. Invest. 113:209.
- Musso, C. et al. (2004) Medicine (Baltimore) 83:209.
Long Name
Insulin Receptor
Alternate Names
CD220, INSR, InsulinR
Gene Symbol
INSR
UniProt
Additional Insulin R/CD220 Products
Product Documents for Recombinant Human Insulin R/CD220 (aa 28-944) Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Insulin R/CD220 (aa 28-944) Protein
For research use only
Related Research Areas
Citations for Recombinant Human Insulin R/CD220 (aa 28-944) Protein
Powered by Bioz
Customer Reviews for Recombinant Human Insulin R/CD220 (aa 28-944) Protein (2)
3 out of 5
2 Customer Ratings
Have you used Recombinant Human Insulin R/CD220 (aa 28-944) Protein?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
2 of
2 reviews
Showing All
Filter By:
-
Application: ELISAVerified Customer | Posted 12/11/2025
-
Application: Western BlotVerified Customer | Posted 01/30/2019Western blot to detect potential protein level changes
There are no reviews that match your criteria.
Loading...
Associated Pathways
Akt Signaling Pathway
MAPK Signaling Pathway: Mitogen Stimulation Pathway
mTOR Signaling Pathway
