Recombinant Human Integrin alpha 11 beta 1 Protein, CF Summary
Optimal dilutions should be determined by each laboratory for each application.
|Human Integrin alpha 11
Accession # Q9UKX5
|Human Integrin beta 1
Accession # P05556
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Integrin alpha 11 beta 1
Integrin alpha 11 beta 1 is a noncovalent, heterodimeric collagen receptor that contains a 150 kDa alpha 11 subunit complexed to a 130 kDa beta 1/CD29 subunit. alpha 11 beta 1 is the only known alpha 11-containing integrin. By contrast, at least twelve alpha -integrins share the beta 1 subunit (1-5). alpha 11 beta 1 is expressed on fibroblasts from cornea, skin and periodontal ligament (3‑9). It is also expressed on mesenchymal stem cells and is considered a marker for non‑muscle mesenchymal tissue (3, 8‑10). Expression of alpha 11 beta 1 is stimulated by TGF‑ beta 1, activin A, and type I interferons, and down‑regulated by FGF-2 (6, 9‑11). The alpha 11 extracellular domain (ECD) contains a vWFA domain which includes the ligand binding site (2‑5). The beta 1 ECD also contains a vWFA domain, which participates in binding. Each subunit has a transmembrane sequence and a short cytoplasmic tail. The dimer assumes a folded configuration at "rest". Divalent cations and intracellular (inside‑out) signaling convert it to an active, extended and open conformation (1, 2). The 1120 amino acid (aa) human alpha 11 ECD shares 90‑93% aa sequence identity with mouse, rat, equine, canine and bovine alpha 11, while the 708 aa human beta 1 ECD shares 92‑96% aa sequence identity with rat, bovine, mouse, and feline beta 1. I domain-containing beta 1 integrins alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1 all bind collagens (3‑5). alpha 11 beta 1 preferentially binds interstitial collagens such as type I (5, 8, 12). It can contract collagen matrices and influence inflammatory edema (3, 13). Depending on context, alpha 11 beta 1 can support or inhibit cell migration on collagen (7, 8). It is frequently up‑regulated in the stroma of lung adenocarcinomas and enhances tumorigenicity (14, 15).
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Citation for Recombinant Human Integrin alpha 11 beta 1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Î±11?1 Integrin is Induced in a Subset of Cancer-Associated Fibroblasts in Desmoplastic Tumor Stroma and Mediates In Vitro Cell Migration
Authors: C Zeltz, J Alam, H Liu, PM Erusappan, H Hoschuetzk, A Molven, H Parajuli, E Cukierman, DE Costea, N Lu, D Gullberg
Cancers (Basel), 2019;11(6):.
Sample Types: In Vivo
Applications: In Vivo
What is the amino acid sequence of the acidic and basic tails?
Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.
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