Recombinant Human Integrin alpha 2 beta 1 Protein, CF

Catalog # Availability Size / Price Qty
5698-A2-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Integrin alpha 2 beta 1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When bovine collagen-II is coated at 10 μg/mL, Recombinant Human Integrin alpha 2 beta 1 binds with an apparent K<1nM.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 2 beta 1 protein
Human Integrin alpha 2
(Tyr30-Thr1132)
Accession # NP_002194
HPGGGSGGGS Human c-Jun
(Arg276-His315)
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556
GGGSGGGS Human c-Fos
(Leu161-Ala199)
N-terminus C-terminus
N-terminal Sequence
Analysis
Tyr30 ( alpha 2) & No results obtained, Gln21 predicted ( beta 1)
Predicted Molecular Mass
126 kDa ( alpha 2) & 83.3 kDa ( beta 1)
SDS-PAGE
140-150 kDa ( alpha 2) & 120-130 kDa ( beta 1), reducing conditions

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5698-A2

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5698-A2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Integrin alpha 2 beta 1

Integrin alpha 2 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 subunit (1-3). It is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83-89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2, while the 708 aa human beta 1 ECD shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I-III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E‑cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8-10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non‑hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).

References
  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
  4. Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
  5. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
  6. Sarratt, K.L. et al. (2005) Blood 106:1268.
  7. Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
  8. Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
  9. McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
  10. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
  11. Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
  12. Edelson, B.T. et al. 2006) Blood 107:143.
Entrez Gene IDs
3673 (Human); 16401 (Mouse)
Alternate Names
Integrin alpha 2 beta 1

Citations for Recombinant Human Integrin alpha 2 beta 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Collagen Type XI Inhibits Lung Cancer-Associated Fibroblast Functions and Restrains the Integrin Binding Site Availability on Collagen Type I Matrix
    Authors: C Zeltz, M Khalil, R Navab, MS Tsao
    International Journal of Molecular Sciences, 2022;23(19):.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Bioassay
  2. Peptostreptococcus anaerobius promotes colorectal carcinogenesis and modulates tumour immunity
    Authors: X Long, CC Wong, L Tong, ESH Chu, C Ho Szeto, MYY Go, OO Coker, AWH Chan, FKL Chan, JJY Sung, J Yu
    Nat Microbiol, 2019;0(0):.
    Species: Bacteria - Peptostreptococcus anaerobius
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice.
    Authors: Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J
    PLoS ONE, 2013;8(11):e81165.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  4. Integrin alpha2beta1 mediates tyrosine phosphorylation of vascular endothelial cadherin induced by invasive breast cancer cells.
    Authors: Haidari M, Zhang W, Caivano A, Chen Z, Ganjehei L, Mortazavi A, Stroud C, Woodside D, Willerson J, Dixon R
    J Biol Chem, 2012;287(39):32981-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What are the functions of the C-Jun and C-Fos peptides included in the Human Integrin Alpha 2 and Human Integrin Beta 1 sequences of Recombinant Human Integrin alpha 2 beta 1 Protein, CF (Catalog # 5698-A2)?

    • The sequences present for C-Jun (Arg276-His315) and C-Fos (Leu161-Ala199) encompass the leucine zipper domains.

      The Integrin alpha 2 and beta 1 subunits are single-pass type 1 membrane proteins. However, Recombinant Human Integrin alpha 2 beta 1 Protein, CF (Catalog # 5698-A2)only expresses the extracellular domains. The inclusion of the leucine zippers form C-Jun and C-Fos allow the alpha 2 and beta 1 subunites to form a non-covalent heterodimer. 

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