Recombinant Human Integrin alpha 2 beta 1 Protein, CF

R&D Systems | Catalog # 5698-A2

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Integrin alpha 2 beta 1 Protein (5698-A2)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_002194 (Integrin alpha 2) & P05556 (Integrin beta 1)

Applications

Binding Activity
View all Integrin alpha 2 beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 2 beta 1 protein
Human Integrin alpha 2
(Tyr30-Thr1132)
Accession # NP_002194		 HPGGGSGGGS Human c-Jun
(Arg276-His315)
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556		 GGGSGGGS Human c-Fos
(Leu161-Ala199)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr30 ( alpha 2) & No results obtained, Gln21 predicted ( beta 1)

Predicted Molecular Mass

126 kDa ( alpha 2) & 83.3 kDa ( beta 1)

SDS-PAGE

140-150 kDa ( alpha 2) & 120-130 kDa ( beta 1), reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When bovine collagen-II is coated at 10 μg/mL, Recombinant Human Integrin alpha 2 beta 1 binds with an apparent Kd <1nM.

Formulation, Preparation, and Storage

5698-A2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 2 beta 1

Integrin alpha 2 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 subunit (1-3). It is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83-89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2, while the 708 aa human beta 1 ECD shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I-III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E‑cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8-10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non‑hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
  4. Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
  5. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
  6. Sarratt, K.L. et al. (2005) Blood 106:1268.
  7. Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
  8. Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
  9. McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
  10. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
  11. Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
  12. Edelson, B.T. et al. 2006) Blood 107:143.

Alternate Names

alpha 2 integrin, alpha 2 subunit of VLA-2 receptor, BR, CD49 antigen-like family member B, CD49b, collagen receptor, glycoprotein Ia, GPIa, HPA-5, human platelet alloantigen 5, human platelet alloantigen system 5, integrin alpha-2, integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor), platelet antigen Br, platelet glycoprotein GPIa, platelet membrane glycoprotein Ia, very late activation protein 2 receptor, alpha-2 subunit, very late activation receptor alpha-2 subunit, very late activation receptor subunit alpha-2, VLA-2, VLAA2

Entrez Gene IDs

3673 (Human); 16401 (Mouse)

Gene Symbol

ITGA2

UniProt

NP_002194 (Integrin alpha 2) & P05556 (Integrin beta 1)

Additional Integrin alpha 2 beta 1 Products

Product Documents for Recombinant Human Integrin alpha 2 beta 1 Protein, CF

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Product Specific Notices for Recombinant Human Integrin alpha 2 beta 1 Protein, CF

For research use only

Citations for Recombinant Human Integrin alpha 2 beta 1 Protein, CF

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FAQs for Recombinant Human Integrin alpha 2 beta 1 Protein, CF

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  • Q: What are the functions of the C-Jun and C-Fos peptides included in the Human Integrin Alpha 2 and Human Integrin Beta 1 sequences of Recombinant Human Integrin alpha 2 beta 1 Protein, CF (Catalog # 5698-A2)?

    A: The sequences present for C-Jun (Arg276-His315) and C-Fos (Leu161-Ala199) encompass the leucine zipper domains.

    The Integrin alpha 2 and beta 1 subunits are single-pass type 1 membrane proteins. However, Recombinant Human Integrin alpha 2 beta 1 Protein, CF (Catalog # 5698-A2)only expresses the extracellular domains. The inclusion of the leucine zippers form C-Jun and C-Fos allow the alpha 2 and beta 1 subunites to form a non-covalent heterodimer. 

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