Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF

R&D Systems | Catalog # 2840-A3

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein (2840-A3)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P26006 (Integrin alpha 3) & P05556 (Integrin beta 1)

Applications

Binding Activity
View all Integrin alpha 3 beta 1/VLA-3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 3 beta 1/VLA-3 protein
Human Integrin alpha 3
(Phe33-Glu991)
Accession # P26006		 acidic tail
Human Integrin beta 1
(Glu21-Asp728)
Accession # P05556		 basic tail
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe33 (Integrin alpha 3) & No results obtained: Gln21 predicted (Integrin beta 1)

Predicted Molecular Mass

110.5 kDa (Integrin alpha 3), 82.3 kDa (Integrin beta 1)

SDS-PAGE

150 kDa and 125 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Collagen IV at 10 µg/mL can bind rhIntegrin a3/ beta 1 with an apparent Kd <2 nM.

Formulation, Preparation, and Storage

2840-A3
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 3 beta 1/VLA-3

VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1-3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha - and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The alpha 3/CD49c subunit is a 130-150 kDa type I TM glycoprotein. It only associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. The human beta 1/CD29 subunit is a 130-140 kDa type I transmembrane glycoprotein (8). Unlike the alpha -subunit, it does not undergo proteolytic cleavage. The molecule contains a 708 aa extracellular region, a 23 aa TM segment, and a 47 aa cytoplasmic domain. The extracellular region contains one von Willebrand factor-like A domain and four cysteine-rich repeats. The cytoplasimc domain shows five alternate splice forms (A, B, C1, C2 and D), all which vary by 12 to 48 amino acids (9, 10). Although the functions of all are unclear, the B isoform appears to be dominant negative to the A isoform (11). Human beta 1 extracellular domain is 92%, 94%, 93% and 96% aa identical to the extracellular regions of beta 1 in rat, bovine, mouse and feline, respectively. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (12).

References

  1. Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
  2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
  3. Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
  4. Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
  5. de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
  6. Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
  7. Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
  8. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
  9. Svineng, G. et al. (1998) Biochem. J. 330:1255.
  10. van der Flier, A. et al. (1995) FEBS Lett. 369:340.
  11. Balzac, F. et al. (1994) J. Cell Biol. 127:557.
  12. Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.

Alternate Names

VLA-3, VLA3

Entrez Gene IDs

3675 (Human)

Gene Symbol

ITGA3

UniProt

P26006 (Integrin alpha 3) & P05556 (Integrin beta 1)

Additional Integrin alpha 3 beta 1/VLA-3 Products

Product Documents for Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF

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Product Specific Notices for Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF

For research use only

Citations for Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF

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FAQs for Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF

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  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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