Recombinant Human Integrin alpha 6 (X1) beta 4 Protein, CF

R&D Systems | Catalog # 5497-A6

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Integrin alpha 6 (X1) beta 4 Protein (5497-A6)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_000201 (Integrin alpha 6) & P16144 (Integrin beta 4)

Applications

Bioactivity
View all Integrin alpha 6 beta 4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 6 beta 4 protein
Human Integrin alpha 6
(Phe24-Lys878)
Accession # NP_000201		 Acidic Tail HHHHHH
Human Integrin beta 4
(Asn28-Ser710)
Accession # P16144		 Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe24 ( alpha 6) & Asn28 ( beta 4)

Predicted Molecular Mass

104 kDa ( alpha 6) & 84.8 kDa ( beta 4)

SDS-PAGE

125-135 kDa ( alpha 6) & 100-110 kDa ( beta 4), reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Mouse Laminin I (Catalog # 3400-010-02) is coated at 10 μg/mL, Recombinant Human Integrin alpha 6 beta 4 binds with an apparent KD <5 nM.

Reviewed Applications

Read 3 reviews rated 4.3 using 5497-A6 in the following applications:

Formulation, Preparation, and Storage

5497-A6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 6 beta 4

Integrin alpha 6 beta 4 is an epithelial and Schwann cell laminin-binding integrin. While alpha 6 can also pair with beta 1, beta 4 pairs only with alpha 6 (1-3). Expression of the non-covalent heterodimer composed of a ~150 kDa alpha 6/CD49f and 150-200 kDa beta 4/CD104 type I transmembrane glycoprotein subunit is required for hemidesmosome formation (2, 4). The alpha 6 subunit contains an I (inhibitory) domain and a cleavage site that creates extracellular heavy and transmembrane light chains. Alternative splicing in the alpha 6 extracellular domain (ECD) at amino acid (aa) 216 creates X1 (ubiquitous), X2 and X1X2 isoforms, while splicing at a cytoplasmic site creates A and B isoforms (5, 6). The cytoplasmic domain of beta 4 is unusually long (~1000 aa) and contains four type III fibronectin repeats that bind intracellular hemidesmosomal components (2, 4, 7). Alternative splicing between repeats # 2 and 3 creates A, B, C and (truncated) D isoforms (7). All ECD variants share similar ligand binding characteristics, while cytoplasmic variants differ in tissue distribution and signaling pathways (5-7). The 683 aa human alpha 6X1 heavy chain shares 94-95% aa identity with mouse, rat, bovine, and canine a6, and the 683 aa human beta 4 ECD shares 87-92% aa identity with mouse, rat, bovine, and equine beta 4. Mutation of alpha 6 beta 4 can cause EB-PA, or epidermolysis bullosa (detachment of epidermis from basement membrane) with pyloric atresia, that is neonatally lethal if severe (8). On Schwann cells, alpha 6 beta 4 cooperates with dystroglycan to stabilize the myelin sheath, and mediates attachment to the basal lamina (9, 10). High alpha 6 beta 4 expression correlates with invasiveness of carcinomas (2). EGF R-induced phosphorylation of beta 4 disrupts hemidesmosomes and allows tumor cell migration (11, 12). alpha 6 beta 4 signaling can also amplify tumor production of VEGF and ErbB proteins (13, 14).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Wilhelmsen, K. et al. (2006) Mol. Cell. Biol. 26:2877.
  3. Nishiuchi, R. et al. (2006) Matrix Biol. 25:189.
  4. Schaapveld, R. Q. J. et al. (1998) J. Cell Biol. 142:271.
  5. Delwel, G. O. et al. (1995) Cell Adhes. Commun. 3:143.
  6. Hogervorst, F. et al. (1993) J. Cell Biol. 121:179.
  7. van Leusden, M. R. et al. (1997) Biochem. Biophys. Res. Commun. 235:826.
  8. Pulkkinen, L. et al. (1998) Am. J. Hum. Genet. 63:1376.
  9. Nodari, A. et al. (2008) J. Neurosci. 28:6714.
  10. Amici, S. A. et al. (2006) J. Neurosci. 26:1179.
  11. Wilhelmsen, K. et al. (2007) Mol. Biol. Cell 18:3512.
  12. Yang, X. H. et al. (2008) Cancer Res. 68:3204.
  13. Lipscomb, E. A. et al. (2005) Cancer Res. 65:10970.
  14. Folgiero, V. et al. (2007) Cancer Res. 67:1645.

Entrez Gene IDs

3655 (Human); 16407 (Mouse)

Gene Symbol

ITGA6

UniProt

NP_000201 (Integrin alpha 6) & P16144 (Integrin beta 4)

Additional Integrin alpha 6 beta 4 Products

Product Documents for Recombinant Human Integrin alpha 6 (X1) beta 4 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Integrin alpha 6 (X1) beta 4 Protein, CF

For research use only

Citations for Recombinant Human Integrin alpha 6 (X1) beta 4 Protein, CF

Customer Reviews for Recombinant Human Integrin alpha 6 (X1) beta 4 Protein, CF (3)

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FAQs for Recombinant Human Integrin alpha 6 (X1) beta 4 Protein, CF

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  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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