Recombinant Human Integrin alpha 9 beta 1 Protein, CF

R&D Systems | Catalog # 5438-A9

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Integrin alpha 9 beta 1 Protein (5438-A9)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q13797 (Integrin alpha 9) & P05556 (Integrin beta 1)

Applications

Bioactivity
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Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 9 beta 1 protein
Human Integrin alpha 9
(Tyr30-Tyr977)
Accession # Q13797		 Acidic Tail HHHHHH
Human Integrin beta 1
(Gln21-Asp728)
Accession # P05556		 Basic Tail
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr30 ( alpha 9) & No results obtained: Gln21 predicted ( beta 1)

Predicted Molecular Mass

114 kDa ( alpha 9) & 86.3 kDa ( beta 1)

SDS-PAGE

130-165 kDa & 150-165 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1.
When 5 x 104 cells per well are added to Recombinant Human Integrin  alpha 9/ beta 1 coated plates (10 µg/mL, 100 µL/well), >50% will adhere after 60 minutes at 37 °C.

Formulation, Preparation, and Storage

5438-A9
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 9 beta 1

Integrin alpha 9 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 (CD29) subunit (1-3). It is the non-covalent heterodimer of 150 kDa alpha 9 and 130 kDa beta 1 type I transmembrane glycoprotein subunits (3). The alpha 9 extracellular domain (ECD) contains an N-terminal beta -propeller structure, followed by domains termed thigh, calf-1 and calf-2 (2). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 9 beta -propeller to form a binding domain when the dimer is in the active, extended and open conformation. The 952 amino acid (aa) human alpha 9 extracellular domain (ECD) shares 89-93% aa sequence identity with mouse, rat, canine, bovine and equine alpha 9, while the 708 aa human beta 1 ECD shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. Each subunit has a transmembrane sequence and a short cytoplasmic tail which, for alpha 9, contains binding sites for paxillin and SSAT (spermine/spermidine-N-acetyltransferase) (4, 5). Integrin alpha 9 beta 1 inhibits cell spreading via signaling through paxillin (4). SSAT interaction with alpha 9 beta 1 allows catalysis that lifts spermine/spermidine inhibition of KIR potassium channels and enhances cell migration (5). Lymphangiogenic vascular endothelia growth factors VEGF-C and VEGF-D are ligands for Integrin alpha 9 beta 1 (6, 7) Genetic deletion of mouse alpha 9 beta 1 results in a fatal chylothorax due to abnormal lymphatic development (8). Integrin alpha 9 beta 1 interacts in cis with G-CSF R and enhances its signals, contributing to granulopoiesis (9). It also binds VCAM-1, which promotes neutrophil trans endothelial migration, and delays apoptosis (10, 11). Other ligands for alpha 9 beta 1 include tenascin, osteopontin, nerve growth factor, and most members of the ADAM family (12, 14).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215. 
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Palmer, E. L. et al. (1993) J. Cell Biol. 123:1289.
  4. Liu, S. et al. (2001) J. Biol. Chem. 276:37086.
  5. deHart, G. W. et al. (2008) Proc. Natl. Acad. Sci. USA 105:7188.
  6. Vlahakis, N. E. et al. (2005) J. Biol. Chem. 280:4544.
  7. Vlahakis, N. E. et al. (2007) J. Biol. Chem. 282:15187.
  8. Huang, X. Z. et al. (2000) Mol. Cell. Biol. 20:5208.
  9. Chen, C. et al. (2006) Immunity 25:895.
  10. Ross, E. A. et al. (2006) Blood 107:1178.
  11. Taooka, Y. et al. (1999) J. Cell Biol. 145:413.
  12. Marcinkiewicz, C. et al. (2000) J. Biol. Chem. 275:31930.
  13. Staniszewska, I. et al. (2008) J. Cell Sci. 121:504.
  14. Eto, K. et al. (2002) J. Biol. Chem. 277:17804.

Entrez Gene IDs

3680 (Human); 104099 (Mouse); 685004 (Rat)

Gene Symbol

ITGA9

UniProt

Q13797 (Integrin alpha 9) & P05556 (Integrin beta 1)

Additional Integrin alpha 9 beta 1 Products

Product Documents for Recombinant Human Integrin alpha 9 beta 1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Integrin alpha 9 beta 1 Protein, CF

For research use only

Citations for Recombinant Human Integrin alpha 9 beta 1 Protein, CF

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FAQs for Recombinant Human Integrin alpha 9 beta 1 Protein, CF

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  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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