Recombinant Human Integrin alpha 9 beta 1 Protein, CF Summary
|Human Integrin alpha 9
Accession # Q13797
|Human Integrin beta 1
Accession # P05556
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Integrin alpha 9 beta 1
Integrin alpha 9 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 (CD29) subunit (1-3). It is the non-covalent heterodimer of 150 kDa alpha 9 and 130 kDa beta 1 type I transmembrane glycoprotein subunits (3). The alpha 9 extracellular domain (ECD) contains an N-terminal beta -propeller structure, followed by domains termed thigh, calf-1 and calf-2 (2). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 9 beta -propeller to form a binding domain when the dimer is in the active, extended and open conformation. The 952 amino acid (aa) human alpha 9 extracellular domain (ECD) shares 89-93% aa sequence identity with mouse, rat, canine, bovine and equine alpha 9, while the 708 aa human beta 1 ECD shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. Each subunit has a transmembrane sequence and a short cytoplasmic tail which, for alpha 9, contains binding sites for paxillin and SSAT (spermine/spermidine-N-acetyltransferase) (4, 5). Integrin alpha 9 beta 1 inhibits cell spreading via signaling through paxillin (4). SSAT interaction with alpha 9 beta 1 allows catalysis that lifts spermine/spermidine inhibition of KIR potassium channels and enhances cell migration (5). Lymphangiogenic vascular endothelia growth factors VEGF-C and VEGF-D are ligands for Integrin alpha 9 beta 1 (6, 7) Genetic deletion of mouse alpha 9 beta 1 results in a fatal chylothorax due to abnormal lymphatic development (8). Integrin alpha 9 beta 1 interacts in cis with G-CSF R and enhances its signals, contributing to granulopoiesis (9). It also binds VCAM-1, which promotes neutrophil trans endothelial migration, and delays apoptosis (10, 11). Other ligands for alpha 9 beta 1 include tenascin, osteopontin, nerve growth factor, and most members of the ADAM family (12, 14).
- Takada, Y. et al. (2007) Genome Biol. 8:215.
- Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
- Palmer, E. L. et al. (1993) J. Cell Biol. 123:1289.
- Liu, S. et al. (2001) J. Biol. Chem. 276:37086.
- deHart, G. W. et al. (2008) Proc. Natl. Acad. Sci. USA 105:7188.
- Vlahakis, N. E. et al. (2005) J. Biol. Chem. 280:4544.
- Vlahakis, N. E. et al. (2007) J. Biol. Chem. 282:15187.
- Huang, X. Z. et al. (2000) Mol. Cell. Biol. 20:5208.
- Chen, C. et al. (2006) Immunity 25:895.
- Ross, E. A. et al. (2006) Blood 107:1178.
- Taooka, Y. et al. (1999) J. Cell Biol. 145:413.
- Marcinkiewicz, C. et al. (2000) J. Biol. Chem. 275:31930.
- Staniszewska, I. et al. (2008) J. Cell Sci. 121:504.
- Eto, K. et al. (2002) J. Biol. Chem. 277:17804.
Citation for Recombinant Human Integrin alpha 9 beta 1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44
Authors: G Fnu, P Agrawal, GC Kundu, GF Weber
Sample Types: Recombinant Protein
What is the amino acid sequence of the acidic and basic tails?
Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.
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