Recombinant Human Integrin alpha V beta 5 Protein, CF

Catalog # Availability Size / Price Qty
2528-AV-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (6)
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Recombinant Human Integrin alpha V beta 5 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Vitronectin (Catalog # 2308-VN) is coated at 1 μg/mL, Recombinant Human Integrin  alpha V beta 5 binds with an apparent KD <0.5 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha V beta 5 protein
Human Integrin alpha V
(Phe31-Val992)
Accession # P06756
His-Pro GGGSGGGS Acidic Tail 6-His tag
Human Integrin beta 5
(Gly24-Asn719)
Accession # P18084
His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus
N-terminal Sequence
Analysis
Phe31 ( alpha V subunit) & Gly24 ( beta 5 subunit)
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
115.4 kDa ( alpha V subunit) & 84.8 kDa ( beta 5 subunit)
SDS-PAGE
100-155 kDa, reducing conditions

Product Datasheets

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2528-AV

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2528-AV

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: Integrin alpha V beta 5

Integrin alpha V beta 5 is one of five alpha V integrins and the only known beta 5 integrin (1-3). The non-covalent heterodimer of 170 kDa alpha V and 100‑110 kDa beta 5 integrin type I transmembrane glycoprotein subunits is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium (4‑8). alpha V beta 5 binds ligands containing an RGD motif, notably vitronectin (1‑10). The 962 aa human alpha V extracellular domain (ECD) shares 92-95% aa sequence identity with mouse, rat and cow alpha V, while the 696 aa human beta 5 ECD shares 89%-93% aa identity with mouse, rat, bovine, equine, and canine beta 5. The alpha V ECD contains an N-terminal beta - propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The 799 aa beta 5 contains a vWFA domain within the ECD, which interacts with the alpha V beta -propeller to form a binding domain. Each subunit has a transmembrane sequence and a short cytoplasmic tail. Potential beta 5 isoforms include a 691 aa form with an alternate start site at aa 109, a 958 aa form with an alternate N‑terminus, and a 795 aa form with an alternate C‑terminus. Post-translational modifications, such as proteolytic cleavage of the alpha V subunit or phosphorylation of the beta 5 cytoplasmic tail, can increase endocytic turnover of the alpha V beta 5 protein and/or promote cell migration (7‑10). Growth factors that increase PKC activity, such as VEGF or TGF‑ alpha, promote alpha V beta 5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF‑ alpha (11). An inhibitor of both down‑regulates tumor angiogenesis (12). During lung inflammation, up‑regulation of alpha V beta 5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF‑ beta from latency (13, 14). On retinal pigment epithelia, alpha V beta 5 is important for normal diurnal phagocytosis of outer rod segments, and contributes to adhesion of retinal cells (15).

References
  1. Hynes, R. O. (2002) Cell 110:673.
  2. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14080.
  3. Suzuki, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5354.
  4. Smith, J.W. et al. (1990) J. Biol. Chem. 265:11008.
  5. Pasqualini, R. et al. (1993) J. Cell Sci. 105:101.
  6. Braam, S.R. et al. (2008) Stem Cells 26:2257.
  7. Memmo, L.M. and P. McKeown-Longo (1998) J. Cell Sci. 111:425.
  8. Kim, J.P. et al. (1994) J. Biol. Chem. 269:26926.
  9. Berthet, V. et al. (2004) FEBS Lett. 557:159.
  10. Li, Z. et al. (2010) Mol. Biol. Cell 21:3317.
  11. Friedlander, M. et al. (1995) Science 270:1500.
  12. Desgrosellier, J.S. and D.A. Cheresh (2010) Nat. Rev. Cancer 10:9.
  13. Wipff, P.J. et al. (2007) J. Cell Biol. 179:1311.
  14. Luzina, I.G. et al. (2009) Arthritis Rheum. 60:1530.
  15. Nandrot, E.F. et al. (2004) J. Exp. Med. 200:1539.
Entrez Gene IDs
3685 (Human)
Alternate Names
CD51; Integrin alpha V beta 5; integrin subunit alpha V; ITGAV; MSK8; VNRA; VTNR

Citations for Recombinant Human Integrin alpha V beta 5 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection
    Authors: M Bieri, R Hendrickx, M Bauer, B Yu, T Jetzer, B Dreier, PRE Mittl, J Sobek, A Plückthun, UF Greber, S Hemmi
    PloS Pathogens, 2021;17(12):e1010083.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Direct targeting of oncogenic RAS mutants with a tumor-specific cytosol-penetrating antibody inhibits RAS mutant-driven tumor growth
    Authors: SM Shin, JS Kim, SW Park, SY Jun, HJ Kweon, DK Choi, D Lee, YB Cho, YS Kim
    Sci Adv, 2020;6(3):eaay2174.
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay
  3. Oral and Subcutaneous Administration of a Near-Infrared Fluorescent Molecular Imaging Agent Detects Inflammation in a Mouse Model of Rheumatoid Arthritis
    Authors: S Bhatnagar, E Khera, J Liao, V Eniola, Y Hu, DE Smith, GM Thurber
    Sci Rep, 2019;9(1):4661.
    Species: Human
    Sample Types: Imaging Agent
    Applications: Binding Assay
  4. Nonimmune cell-derived ICOS ligand functions as a renoprotective alphavbeta3 integrin-selective antagonist
    Authors: KH Koh, Y Cao, S Mangos, NJ Tardi, RR Dande, HW Lee, B Samelko, MM Altintas, VP Schmitz, H Lee, K Mukherjee, V Peev, DJ Cimbaluk, J Reiser, E Hahm
    J. Clin. Invest., 2019;129(4):1713-1726.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  5. Systematic site-directed mutagenesis of the Helicobacter pylori CagL protein of the Cag type IV secretion system identifies novel functional domains
    Sci Rep, 2016;6(0):38101.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Developmet
  6. Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice.
    Authors: Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J
    PLoS ONE, 2013;8(11):e81165.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Binding Assay

FAQs

  1. What is the amino acid sequence of the acidic and basic tails?

    • Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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