Recombinant Human ITIH4 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10269-IT-050
ITIH4 Protein Bioactivity
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Product Details
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Recombinant Human ITIH4 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human ITIH4 is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human TSG‑6 (Catalog # 2104-TS) that produces 50% of the optimal binding response is 5-25 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human ITIH4 protein
Glu29-Leu930, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu29
Predicted Molecular Mass
101 kDa
SDS-PAGE
113-117 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10269-IT

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human ITIH4 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 115 kDa under reducing conditions.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ITIH4

Inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4), also known as plasma kallikrein sensitive glycoprotein 120 (PK-120 or gp120), is a single chain protein that is synthesized mainly in liver and circulates at high levels in the plasma (1). ITIH4 can be processed by plasma kallikrein to 35kDa and 70 kDa fragments (1). ITIHs can be incorporated into inter-alpha-inhibitor complex with two HC linked to bikunin via chondroition sulfate (2,3) or associated with hyaluronan (HA) (3). Although the N-terminal 70kD fragment of ITIH4 contains a von-Willebrand type A domain and displays significant sequence similarity with other ITIHs (2,4), ITIH4 is the only member of the family with a kallikrein-sensitive domain in the c-terminus (1) and is not capable of complexing with bikunin (2,5). The unprocessed or 70kD fragment may bind to HA leading to stabilization of the extracellular matrix as for other HC (1,6). ITIH4 is up-regulated in acute inflammation (7) through interleukins (8). The 35kDa fragment is overexpressed in oestrogen-related conditions of select cancers and pregnancy (9) where increased plasma kallikrein can lead to cleavage and increased expression of pro-inflammatory cytokines while the long isoform of ITIH leads to upregulation of anti-inflammatory cytokines (10). ITIH4 is implicated as a diagnostic and prognostic marker in several diseases including hepatocellular carcinoma with NAFLD (11), acute lymphoblastic leukemia (12), colorectal adenoma (13) and Alzheimer's (14).

References
  1. Nishimura, H. et al. (1995) FEBS Lett. 357:207.
  2. Salier, JP et al. (1996) Biochem. J. 315:1.
  3. Zhuo, L. et al. (2004) J. Biol. Chem. 279:38079.
  4. Jean, L. et al. (1997) Genomics 41:139.
  5. Hashimoto, K. et al. (1996) J. Biochem. 119:577.
  6. Baranova. N.S et al. (2013) J. Biol. Chem. 288:29642.
  7. Gonzalez-Ramon, N. et al. (1995) FEBS Lett. 371:227.
  8. Daveau, M. et al. (1993) Biochem. J. 292:485.
  9. Mohamed, E. et al. (2013) Biomarker Research 1:19.
  10. Li, L. et al. (2018) EBioMedicine 37:535.
  11. Nakamura, N. et al. (2019) BMC Cancer 19:621.
  12. Guo, L. et al. (2019) Onco. Targets Ther. 12:3859.
  13. Ivancic, M.M. et al. (2019) Proc. Natl. Acad. Sci. USA 116:8471.
  14. Shi, X. et al. (2019) J. Alzheimers Dis. 68:1667.
Long Name
Inter-alpha-Trypsin Inhibitor Heavy Chain H4
Entrez Gene IDs
3700 (Human); 16427 (Mouse); 54404 (Rat)
Alternate Names
DKFZp686G21125; gp120; H4P; heavy chain-like, 1; IHRP; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein); inter-alpha-trypsin inhibitor family heavy chain-related protein; inter-alpha-trypsin inhibitor heavy chain H4; ITIH4; ITI-HC4; ITIHL1; PK120; PK-120; plasma kallikrein-sensitive glycoprotein 120; PRO1851

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