Recombinant Human ITIH4 His-tag Protein, CF Summary
Glu29-Leu930, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human ITIH4 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 115 kDa under reducing conditions.
Inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4), also known as plasma kallikrein sensitive glycoprotein 120 (PK-120 or gp120), is a single chain protein that is synthesized mainly in liver and circulates at high levels in the plasma (1). ITIH4 can be processed by plasma kallikrein to 35kDa and 70 kDa fragments (1). ITIHs can be incorporated into inter-alpha-inhibitor complex with two HC linked to bikunin via chondroition sulfate (2,3) or associated with hyaluronan (HA) (3). Although the N-terminal 70kD fragment of ITIH4 contains a von-Willebrand type A domain and displays significant sequence similarity with other ITIHs (2,4), ITIH4 is the only member of the family with a kallikrein-sensitive domain in the c-terminus (1) and is not capable of complexing with bikunin (2,5). The unprocessed or 70kD fragment may bind to HA leading to stabilization of the extracellular matrix as for other HC (1,6). ITIH4 is up-regulated in acute inflammation (7) through interleukins (8). The 35kDa fragment is overexpressed in oestrogen-related conditions of select cancers and pregnancy (9) where increased plasma kallikrein can lead to cleavage and increased expression of pro-inflammatory cytokines while the long isoform of ITIH leads to upregulation of anti-inflammatory cytokines (10). ITIH4 is implicated as a diagnostic and prognostic marker in several diseases including hepatocellular carcinoma with NAFLD (11), acute lymphoblastic leukemia (12), colorectal adenoma (13) and Alzheimer's (14).
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