Recombinant Human JAM-A Fc Chimera Protein, CF Summary
(Ser28 - Ala242)
Accession # Q9Y624
|IEGRMD||Human IgG1 |
(Pro100 - Lys330)
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-A, also known as platelet adhesion molecule 1 (PAM-1) and platelet F11 receptor (3), is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (1 - 4). It is also expressed on circulating blood cells including neutrophils, monocytes, platelets, erythrocytes and lymphocytes (5). Human JAM-A cDNA predicts a 299 amino acid (aa) residue precursor protein with a putative 27 aa signal peptide, a 210 aa extracellular region containing two Ig-like V-subset domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. The human and mouse proteins share approximately 67% aa sequence homology. Human JAM-A also shares approximately 35% and 32% aa sequence homology with human JAM-B and JAM-C, respectively. JAM-A exhibits homophilic interactions to regulate tight junction assembly and modulate paracellular permeability. This homophilic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (3). JAM-A binds heterotypically with the beta 2 integrin lymphocyte function-associated antigen-1 (LFA-1). This JAM-A-LFA-1 interaction is involved in leukocyte adhesion and transmigration (6). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (7).
- Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
- Aurand-Lions, M. et al. (2001) Blood 98:3699.
- Sobocka, M.B. et al. (2000) Blood 95:2600.
- Martin-Padura, I. et al. (1998) J. Cell Biol. 142:117.
- Williams, L.A. et. al. (1999) Mol. Immunol. 36:1175.
- Ostermann, G. et al. (2002) Nature Immunol. 3:151.
- Barton, E.S. et al. (2001) Cell 104:441.
Citations for Recombinant Human JAM-A Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Development of near-infrared imaging agents for detection of junction adhesion molecule-A protein
Authors: E Walker, SM Turaga, X Wang, R Gopalakris, S Shukla, JP Basilion, JD Lathia
Translational Oncology, 2021;14(3):101007.
Sample Types: Whole Cells
JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals.
Authors: Williams D, Anastos K, Morgello S, Berman J
J Leukoc Biol, 2015;97(2):401-12.
Sample Types: Plasma
Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
Authors: Haarmann A, Deiss A, Prochaska J, Foerch C, Weksler B, Romero I, Couraud PO, Stoll G, Rieckmann P, Buttmann M
PLoS ONE, 2010;5(10):e13568.
Sample Types: N/A
Applications: ELISA (Standard)
Regulated release and functional modulation of junctional adhesion molecule A by disintegrin metalloproteinases.
Authors: Koenen RR, Pruessmeyer J, Soehnlein O, Fraemohs L, Zernecke A, Schwarz N, Reiss K, Sarabi A, Lindbom L, Hackeng TM, Weber C, Ludwig A
Sample Types: Recombinant Protein
Applications: Enzyme Assay
Sushi repeat protein X-linked 2, a novel mediator of angiogenesis.
Authors: Miljkovic-Licina M, Hammel P, Garrido-Urbani S, Bradfield PF, Szepetowski P, Imhof BA
FASEB J., 2009;23(12):4105-16.
Sample Types: Cell Lysates
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