Recombinant Human Kallikrein 2 His-tag Protein, CF

R&D Systems | Catalog # 11691-SE

CHO expressed
R&D Systems
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Kallikrein 2 His-tag Protein (11691-SE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P20151.1

Applications

Enzyme Activity
View all Kallikrein 2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Kallikrein 2 protein
Ile25-Pro261, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile25

Predicted Molecular Mass

28 kDa

SDS-PAGE

22-32 kDa, under non-reducing conditions

Activity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC).
The specific activity is >250 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human Kallikrein 2 His-tag Protein, CF

Recombinant Human Kallikrein 2 His-tag Protein Enzyme Activity.

Recombinant Human Kallikrein 2 His-tag (Catalog # 11691-SE) is measured by its ability to cleave the fluorogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC).

Formulation, Preparation, and Storage

11691-SE
Formulation Supplied as a 0.2 μm filtered solution in Sodium Citrate and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Kallikrein 2

Recombinant human Kallikrein 2 (KLK2) is also known as glandular kallikrein or the prostate-specific glandular kallikrein as it is structurally related to KLK3, the prostate-specific antigen (PSA). KLK2 is a secreted serine protease member of the tissue kallikrein subfamily family of the peptidase S1 family (1). It is highly expressed in the human prostate gland (2) and synthesized as a pre-proenzyme like other kallikriens. Removal of the propeptide occurs through cleavage by trypsin-like activity to activate the protein (1).  KLK2 also contains a catalytic domain with an extended 99 or kallikrein loop (3) and glycan that regulate its activity (4). KLK2 is highly specific for cleavage after arginine residues and is able to autoactivate and play a role in activation of KLK cascades (5). In addition, KLK2 has been reported to activate other key proteins including the urokinase-type plasminogen activator (6), IGFBPs (7), and IL10 (8). Its activity is highly regulated by activation cascades, endogenous inhibitors such as serpins protein C inhibitor, antichymotrypsin, and plasminogen activator inhibitor 1 (9-11), pH, and metal dependency (1). It is heavily implicated to play a role in prostate cancer and is attractive as a biomarker and therapeutic target (12-15). 

References

  1. Prassas, I. et al. (2015) Nat. Rev. 14:183. 
  2. Chapdelaine, P. et al. (1988) FEBS Lett. 236:205.
  3. Skala, W. et al. (2014) J. Biol. Chem. 289:34267.
  4. Guo, S. et al. (2016) J. Biol. Chem. 291:593. 
  5. Yoon, H. et al. (2007) J. Biol. Chem. 282:31852.
  6. Frenette, G. et al. (1997) Int. J. Cancer 71:897.
  7. Emami, N. and E.P. Diamandis (2007) Mol. Oncol. 1:269.
  8. Oliveira, J.R. et al. (2024) Biochemistry 63:2023.
  9. Deperthes, D. et al. (1995) Biochim. Biophys. Acta 1245:311.
  10. Grauer, L.S. et al. (1998) J. Androl. 19:407.
  11. Mikolajczyk S.D. et al. (1999) Cancer Res. 59:3927.
  12. Sardana, G. et al. (2008) Clin. Chem. 54:1951.
  13. Shang, Z. et al. (2014) Tumor Biol. 35:1881.
  14. Bonk, S. et al. (2020) Prostate 80:1097.
  15. Zhang, J. and J.S. Chadha (2024) Cancers 16:3098.

Alternate Names

Klk1c2, KLK2, KLK2A2, Ton

Entrez Gene IDs

3817 (Human); 24841 (Rat); 102139474 (Cynomolgus Monkey)

Gene Symbol

KLK2

UniProt

P20151.1

Additional Kallikrein 2 Products

Product Documents for Recombinant Human Kallikrein 2 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Kallikrein 2 His-tag Protein, CF

For research use only

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Protocols

View specific protocols for Recombinant Human Kallikrein 2 His-tag Protein, CF (11691-SE):

Materials
  • Assay Buffer: 50 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 7.5 (TCNB) 
  • Recombinant Human Kallikrein 2 (rhKLK2) (Catalog # 11691-SE) 
  • Substrate: Pro-Phe-Arg-AMC, 10 mM stock in DMSO
  • Black 96-well plate
  • Plate Reader with Fluorescence Read Capability
  1. Dilute rhKLK2 to 4 µg/mL in Assay Buffer.  
  2. Dilute Substrate to 200 µM in Assay Buffer.  
  3. In a plate, load 50 µL of 4 µg/mL rhKLK2, and start the reaction by adding 50 µL of the 200 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 200 µM Substrate. 
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity: 

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

    

*Adjusted for Substrate Blank      
**Derived using calibration standard 7-Amino, 4-Methyl Coumarin. 
Per Well:
  • rhKLK2: 0.2 µg  
  • Substrate: 100 µM
























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