Recombinant Human Kallikrein 2 Protein, CF

R&D Systems | Catalog # 4104-SE

New Version Available: 11691-SE
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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Kallikrein 2 Protein (4104-SE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P20151

Applications

Enzyme Activity
View all Kallikrein 2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Kallikrein 2 protein
Met1-Pro261, with a C-terminal 10-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile25 & Leu124

Predicted Molecular Mass

28 kDa & 16 kDa

SDS-PAGE

34 kDa & 15-18 kDa, reducing conditions

Activity

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC).
The specific activity is >125 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation, and Storage

4104-SE
Formulation Supplied as a 0.2 μm filtered solution in Sodium Citrate and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Kallikrein 2

Kallikrein 2 (KLK2) is a secreted serine protease that is highly expressed in the human prostate gland (1). It is also known as the prostate-specific glandular kallikrein. The enzyme is highly specific for cleavage after arginine residues. KLK2 is able to activate the urokinase-type plasminogen activator (2). KLK2 is inhibited by serpins such as protein C inhibitor, antichymotrypsin, and plasminogen activator inhibitor 1 (3 ‑ 5). KLK2 is structurally related to KLK3, the prostate-specific antigen (PSA). Like PSA, KLK2 is considered to be a biomarker for prostate cancer (6).

References

  1. Chapelaine, P. et al. (1988) FEBS Lett. 236:205.
  2. Frenette, G. et al. (1997) Int. J. Cancer 71:897.
  3. Deperthes, D. et al. (1995) Biochim. Biophys. Acta 1245:311.
  4. Grauer, L.S. et al. (1998) J. Androl. 19:407.
  5. Mikolajczyk S.D. et al. (1999) Cancer Res. 59:3927.
  6. Sardana, G. et al. (2008) Clin. Chem. 54:1951.

Alternate Names

Klk1c2, KLK2, KLK2A2, Ton

Entrez Gene IDs

3817 (Human); 24841 (Rat); 102139474 (Cynomolgus Monkey)

Gene Symbol

KLK2

UniProt

P20151

Additional Kallikrein 2 Products

Product Documents for Recombinant Human Kallikrein 2 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Kallikrein 2 Protein, CF

For research use only

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Protocols

View specific protocols for Recombinant Human Kallikrein 2 Protein, CF (4104-SE):

Materials
  • Assay Buffer: 50 mM Tris, 0.15 M NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 7.5 (TCNB)
  • Recombinant Human Kallikrein 2 (rhKLK2) (Catalog # 4104-SE)
  • Substrate: Pro-Phe-Arg-AMC (Bachem, Catalog # I-1295), 10 mM stock in DMSO
  • 96-well black plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhKLK2 to 4 µg/mL in Assay Buffer.
  2. Dilute Substrate to 200 µM in Assay Buffer.
  3. In a plate, load 50 µL of 4 µg/mL rhKLK2, and start the reaction by adding 50 µL of the 200 µM Substrate. As a Substrate Blank combine 50 µL of Assay Buffer with 50 µL of the 200 µM Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 0 to 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-Amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).

Per Well:
  • rhKLK2: 0.2 µg
  • Substrate: 100 µM

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