Kallikrein 8 (KLK8), also known as neuropsin or ovasin, is a member of the human tissue kallikrein family (1). Two alternatively spliced forms exist, resulting in 260 (isoform 1) and 305 (isoform 2) amino acid sequences, respectively (2). Isoform 1 consists of a signal peptide (residues 1 to 28), a short pro peptide (residues 29 to 32) and the mature chain (residues 33 to 260). Isoform 2 is identical to isoform 1, except that a 45 amino acid segment is inserted in isoform 2 between residues 23 and 24 in isoform 1. Isoform 1 is predominantly expressed in pancreas whereas isoform 2 is preferentially expressed in adult brain and hippocampus, although both forms are expressed in fetal brain and placenta in comparable levels. The brain function of KLK8 seems evident in neuropsin knockout mice that showed abnormalities of synapses and neurons and predisposation to global seizure activity (3, 4). KLK8 is a novel marker for ovarian and cervical cancer carcinomas (5, 6). Recombinant human KLK8, after being activated by lysyl endopeptidase, can cleave fibronectin and several small peptide substrates (7, 8). This activity can be inhibited by recombinant human Serpin A5, recombinant human Serpin F2 and AEBSF (Catalog # http://www.rndsystems.com/product_results.aspx?k=1266-PI">1266-PI, http://www.rndsystems.com/product_results.aspx?k=1470-PI">1470-PI and http://www.rndsystems.com/product_results.aspx?k=EI001">EI001, respectively). Recombinant human KLK8 produced by R&D Systems corresponds to isoform 1.
Recombinant Human Kallikrein 8/Neuropsin Protein, CF
R&D Systems | Catalog # 2025-SE
Loading...
Key Product Details
- R&D Systems NS0-derived Recombinant Human Kallikrein 8/Neuropsin Protein (2025-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Pro form
Applications
Enzyme Activity
Loading...
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Kallikrein 8/Neuropsin protein
Gln29-Gly260, with a C-terminal 10-His tag
Gln29-Gly260, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln29 predicted
Predicted Molecular Mass
26 kDa
SDS-PAGE
37-39 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).
The specific activity is >500 pmol/min/µg, as measured under the described conditions.
The specific activity is >500 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
2025-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Kallikrein 8/Neuropsin
References
- Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
- Mitsui, S. et al. (1999) Eur. J. Biochem. 260:627.
- Hirata, A. et al. (2001) Mol. Cell. Neurosci. 17:600.
- Davies, B. et al. (2001) J. Neurosci. 21:6993.
- Kishi, T. et al. (2003) Cancer Res. 63:2771.
- Cane, S. et al. (2004) Am. J. Obstet. Gynecol. 190:60.
- Oka, T. et al. (2002) J. Biol. Chem. 277:14724.
- Shimizu, C. et al. (1998) J. Biol. Chem. 273:11189.
Alternate Names
KLK8, Neuropsin, Ovasin, PRSS19
Entrez Gene IDs
11202 (Human)
Gene Symbol
KLK8
UniProt
Additional Kallikrein 8/Neuropsin Products
Product Documents for Recombinant Human Kallikrein 8/Neuropsin Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Kallikrein 8/Neuropsin Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human Kallikrein 8/Neuropsin Protein, CF
Powered by Bioz
Customer Reviews for Recombinant Human Kallikrein 8/Neuropsin Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Kallikrein 8/Neuropsin Protein, CF and earn rewards!
Have you used Recombinant Human Kallikrein 8/Neuropsin Protein, CF?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
View specific protocols for Recombinant Human Kallikrein 8/Neuropsin Protein, CF (2025-SE):
Materials
- Activation Buffer: 50 mM Tris, pH 8.0
- Assay Buffer: 50 mM Tris, pH 9.0
- Recombinant Human Kallikrein 8/Neuropsin (rhKLK8) (Catalog # 2025-SE)
- Lysyl-Endopeptidase, 20 U/mL stock in PBS
- Substrate: BOC-Val-Pro-Arg-AMC (Catalog # ES011), 10 mM stock in DMSO
- Black 96-well Plate
- Plate Reader with Fluorescence Read Capability
- Dilute rhKLK8 to 200 µg/mL in Activation Buffer.
- Dilute Lysyl-Endopeptidase to 0.4 mU/mL in Activation Buffer.
- Activate rhKLK8 by combining equal volumes of diluted rhKLK8 and Lysyl-Endopeptidase.
- Incubate at 37 °C for 30 minutes.
- Dilute activated rhKLK8 to 1 ng/µL in Assay Buffer.
- Dilute Substrate to 200 µM in Assay Buffer.
- Load 50 µL of 1 ng/µL rhKLK8 into the plate, and start the reaction by adding 50 µL of 200 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 200 µM Substrate.
- Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 7-Amino, 4-Methyl Coumarin (AMC).
Per Well:
- rhKLK8: 0.050 µg
- Substrate: 100 µM
Loading...