Recombinant Human KGF/FGF-7 Protein, CF

R&D Systems | Catalog # BT-KGF

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human KGF/FGF-7 Protein (BT-KGF)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P21781.1

Applications

Bioactivity
View all KGF/FGF-7 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human KGF/FGF-7 protein
Cys32-Thr194, with an N-terminal Met

Purity

>97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys32 & Asn33

Predicted Molecular Mass

19 kDa

SDS-PAGE

20 kDa, reducing conditions.

Activity

Measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb.
The ED50 for this effect is 6.00-60.0 ng/mL.

Scientific Data Images for Recombinant Human KGF/FGF-7 Protein, CF

Recombinant Human KGF/FGF‑7 Protein SEC-MALS.

Recombinant Human KGF/FGF‑7 Protein (Catalog # BT-KGF) has a molecular weight (MW) of 24.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.

Recombinant Human KGF/FGF‑7 Protein Bioactivity.

Recombinant Human KGF/FGF‑7 Protein (Catalog # BT-KGF) induced cell proliferation in Ba/F3 mouse pro-B cells transfected with human FGF RIIb. The ED50 for this effect is 6.00-60.0 ng/mL.

Recombinant Human KGF/FGF‑7 Protein SDS-PAGE.

2 μg/lane of Recombinant Human KGF/FGF‑7 Protein (Catalog # BT-KGF) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 20 kDa.

Formulation, Preparation, and Storage

BT-KGF
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with Trehalose.
Reconstitution Reconstitute the 20 μg size at 200 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KGF/FGF-7

Keratinocyte Growth Factor (KGF), or Fibroblast Growth Factor-7 (FGF-7), is an important component in many cell culture protocols. It is widely used to promote the growth and differentiation of epithelial cells, including skin keratinocytes, and lung, corneal, and intestinal epithelia. It is a part of tissue engineering and organoid culture protocols, where it can enhance the generation of complex tissues such as liver, gastrointestinal tract, lung, and mammary gland. Furthermore, KGF/FGF-7 has been shown to stimulate the proliferation of pancreatic endocrine progenitor cells and promote their differentiation into mature insulin-secreting beta cells. In these capacities KGF/FGF-7 has significant clinical relevance in studies to better understand gastrointestinal disorders, wound healing, respiratory disease, and diabetes. 

Long Name

Keratinocyte Growth Factor

Alternate Names

FGF-7, FGF7, HBGF-7, HBGF7

Entrez Gene IDs

2252 (Human); 403915 (Canine)

Gene Symbol

FGF7

UniProt

P21781.1

Additional KGF/FGF-7 Products

Product Documents for Recombinant Human KGF/FGF-7 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human KGF/FGF-7 Protein, CF

For research use only

Related Research Areas

FGF Family
FGF Ligands
gamma delta T Cells

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