Recombinant Human KGF/FGF-7 Protein, CF
Recombinant Human KGF/FGF-7 Protein, CF Summary
- R&D Systems E. coli-derived Recombinant Human KGF/FGF-7 Protein (BT-KGF)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Cys32-Thr194, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
BT-KGF
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with Trehalose. |
| Reconstitution | Reconstitute the 20 μg size at 200 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human KGF/FGF‑7 Protein (Catalog # BT-KGF) has a molecular weight (MW) of 24.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
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Recombinant Human KGF/FGF‑7 Protein (Catalog # BT-KGF) induced cell proliferation in Ba/F3 mouse pro-B cells transfected with human FGF RIIb. The ED50 for this effect is 6.00-60.0 ng/mL.
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2 μg/lane of Recombinant Human KGF/FGF‑7 Protein (Catalog # BT-KGF) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 20 kDa.
Reconstitution Calculator
Background: KGF/FGF-7
Keratinocyte Growth Factor (KGF), or Fibroblast Growth Factor-7 (FGF-7), is an important component in many cell culture protocols. It is widely used to promote the growth and differentiation of epithelial cells, including skin keratinocytes, and lung, corneal, and intestinal epithelia. It is a part of tissue engineering and organoid culture protocols, where it can enhance the generation of complex tissues such as liver, gastrointestinal tract, lung, and mammary gland. Furthermore, KGF/FGF-7 has been shown to stimulate the proliferation of pancreatic endocrine progenitor cells and promote their differentiation into mature insulin-secreting beta cells. In these capacities KGF/FGF-7 has significant clinical relevance in studies to better understand gastrointestinal disorders, wound healing, respiratory disease, and diabetes.
FAQs
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