Recombinant Human KIR2DL5/CD158f Fc Chimera Protein, CF

R&D Systems | Catalog # 6634-KR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human KIR2DL5/CD158f Fc Chimera Protein (6634-KR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_065396

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all KIR2DL5/CD158f Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human KIR2DL5/CD158f protein
Human KIR2DL5
(His22 - His240)
Accession # NP_065396		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His22

Predicted Molecular Mass

50.1 kDa (monomer)

SDS-PAGE

66 kDa, reducing conditions

Activity

Measured by its ability to bind HLA on MDA‑MB‑231 human breast cancer cells.

The ED50 for this effect is 0.9-3.6 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.

Reviewed Applications

Read 2 reviews rated 4.5 using 6634-KR in the following applications:

Formulation, Preparation, and Storage

6634-KR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KIR2DL5/CD158f

KIR2DL5 (2DL5, designated CD158f) is a 60 kDa type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family (1, 2). KIRs are expressed on CD56dim NK cells and T cell subsets where they regulate effector functions in the innate immune system (1 - 3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails (3). Like other inhibiting KIRs, KIR2DL5 has two ITIM domains within its long tail; however, one of these is atypical and may cause KIR2DL5 to be less inhibitory than most inhibiting KIRs (4). Gene duplication is thought to have resulted in individual genes coding for KIR2DL5 A and B proteins that vary by only 2 amino acids (5). Individuals may express either KIR2DL5A or KIR2DL5B, both proteins, or neither protein (5). Within the KIR family, KIR2DL5 and KIR2DL4 form a subfamily. The two share 79% amino acid sequence identity, have longer cytoplasmic tails than other KIR, and each has one D0 and one D2-type Ig-like domain (1). However, KIR2DL5 is only inhibitory, while KIR2DL4 has characteristics of both activating and inhibiting KIR.

References

  1. Vilches, C. et al. (2000) J. Immunol. 164:5797.
  2. Estefania, E. et al. (2007) J. Immunol. 178:4402.
  3. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  4. Yusa, S. et al. (2004) J. Immunol. 172:7385.
  5. Gomez-Lozano, N. et al. (2002) Immunogenetics 54:314.

    Long Name

    Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 5

    Alternate Names

    CD158f, KIR2DL5A

    Entrez Gene IDs

    57292 (Human)

    Gene Symbol

    KIR2DL5A

    UniProt

    NP_065396

    Additional KIR2DL5/CD158f Products

    Product Documents for Recombinant Human KIR2DL5/CD158f Fc Chimera Protein, CF

    Certificate of Analysis

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    Note: Certificate of Analysis not available for kit components.

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    Product Specific Notices for Recombinant Human KIR2DL5/CD158f Fc Chimera Protein, CF

    For research use only

    Citations for Recombinant Human KIR2DL5/CD158f Fc Chimera Protein, CF

    Customer Reviews for Recombinant Human KIR2DL5/CD158f Fc Chimera Protein, CF (2)

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      Application: Binding assay/Protein-protein interaction
      Verified Customer | Posted 11/30/2018

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