Recombinant Human KIR2DL5/CD158f Fc Chimera Protein, CF Summary
The ED50 for this effect is 0.9-3.6 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
(His22 - His240)
Accession # NP_065396
(Pro100 - Lys330)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 300 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
KIR2DL5 (2DL5, designated CD158f) is a 60 kDa type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family (1, 2). KIRs are expressed on CD56dim NK cells and T cell subsets where they regulate effector functions in the innate immune system (1 - 3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails (3). Like other inhibiting KIRs, KIR2DL5 has two ITIM domains within its long tail; however, one of these is atypical and may cause KIR2DL5 to be less inhibitory than most inhibiting KIRs (4). Gene duplication is thought to have resulted in individual genes coding for KIR2DL5 A and B proteins that vary by only 2 amino acids (5). Individuals may express either KIR2DL5A or KIR2DL5B, both proteins, or neither protein (5). Within the KIR family, KIR2DL5 and KIR2DL4 form a subfamily. The two share 79% amino acid sequence identity, have longer cytoplasmic tails than other KIR, and each has one D0 and one D2-type Ig-like domain (1). However, KIR2DL5 is only inhibitory, while KIR2DL4 has characteristics of both activating and inhibiting KIR.
- Vilches, C. et al. (2000) J. Immunol. 164:5797.
- Estefania, E. et al. (2007) J. Immunol. 178:4402.
- Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
- Yusa, S. et al. (2004) J. Immunol. 172:7385.
- Gomez-Lozano, N. et al. (2002) Immunogenetics 54:314.
Citation for Recombinant Human KIR2DL5/CD158f Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
HIV-1 Nef-mediated downregulation of CD155 results in viral restriction by KIR2DL5+ NK cells
Authors: P Fittje, A Hœlzemer, WF Garcia-Bel, S Vollmers, A Niehrs, K Hagemann, G Martrus, C Körner, F Kirchhoff, D Sauter, M Altfeld
Sample Types: Recombinant Proteins
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