Recombinant Human KIR3DL1 Fc Chimera Protein, CF

(1 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to bind HLA on MDA‑MB‑231 human breast cancer cells. The ED50 for this effect is 0.02-0.12 μg/mL.
    Optimal dilutions should be determined by each laboratory for each application.
  • Source
    Mouse myeloma cell line, NS0-derived
    Human KIR3DL1
    His22-His340 (Met23Val)
    Accession #P43629
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    His22
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    61.7 kDa (monomer)
  • SDS-PAGE
    70-90 kDa, reducing conditions
1225-KR
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human KIR3DL1 Fc Chimera (Catalog # 1225-KR) binds to MDA-MB-231 human breast cancer cells. The ED50 for this effect is 0.02-0.12 μg/mL.
Background: KIR3DL1

KIR3DL1 (3DL1, previously called NKB1 or NKAT3, designated CD158e) is a 70 kDa type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. KIRs are expressed on CD56dim NK cells and T cell subsets where they regulate effector functions in the innate immune system (1 - 3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. Like other inhibiting KIRs, KIR3DL1 has two ITIM domains within its long tail (2). The 319 amino acid (aa) ECD of KIR3DL1 shows 97% aa identity with an activating KIR, KIR3DS1, and the two segregate as alleles (3, 4). KIR3DL1 binds to HLA antigens. This includes HLA-A and -B molecules. Among the HLA-B variants, only the Bw4 epitope, which is present within only one third of all HLA-B alleles, is recognized by KIR3DL1 (4). An NK cell expressing KIR3DL1 is prevented from killing a cell expressing the Bw4 epitope on its surface. However, if the epitope is downregulated on the cell surface due to viral infection, the NK cell is released from inhibition and now kills the infected cell. KIR genes are highly polymorphic, and specific KIR3DL1 alleles vary in surface expression and activity. For example, the allele KIR3DL1*004 is associated with slow progression to AIDS in HIV infected individuals that also express Bw4 (6). Unlike most alleles that are surface-expressed, this allele is mainly retained within the cell (7). KIR3DL1/S1 is the only KIR receptor to have an ortholog in non-primates, including selected mouse strains in which it is also called KIRL1 (KIR-like 1). Although the ECD of human KIR3DL1 shares 40 - 48% aa identity with mouse, rat and bovine KIR3DL1, the transmembrane and cytoplasmic regions in the non-primate species show no obvious activating or inhibiting motifs (8, 9).

  • References:
    1. Colonna, M. and J. Samaridis (1995) Science 268:405.
    2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
    3. Uhrberg, M. et al. (1997) Immunity 7:753.
    4. O’Connor, G. M. et al. (2007) J. Immunol. 178:235.
    5. Thananchai, H. et al. (2007) J. Immunol. 178:33.
    6. Martin, M.P. et al. (2007) Nat. Genet. 39:733.
    7. Pando, M.J. et al. (2003) J. Immunol. 171:6640.
    8. Hoelsbrekken, S.E. et al. (2003) J. Immunol. 170:2259.
    9. Wilson, E.B. et al. (2007) Immunogenetics 59:641.
  • Long Name:
    Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 1
  • Entrez Gene IDs:
    3811 (Human)
  • Alternate Names:
    AMB11; CD158 antigen-like family member E; CD158e antigen; CD158E; CD158e1; CD158e1/2; CD158e2; cl-11; cl-2; HLA-BW4-specific inhibitory NK cell receptor; killer cell immunoglobulin-like receptor 3DL1; killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail1,nkat3; killer Ig receptor; KIR antigen 3DL1; KIR3DL1; KIR3DL1/S1; KIR3DS1; MGC119726; MGC119728; MGC126589; MGC126591; MHC class I NK cell receptor; Natural killer-associated transcript 3; Nkat3; NKAT-3; NKAT3KIR; NKB1; NKB1B; NKB1CD158E1; NK-receptor; p70 killer cell inhibitory receptor; p70 natural killer cell receptor clones CL-2/CL-11; p70 NK receptor CL-2/CL-11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. ERAP1 regulates natural killer cell function by controlling the engagement of inhibitory receptors.
    Authors: Cifaldi L, Romania P, Falco M, Lorenzi S, Meazza R, Petrini S, Andreani M, Pende D, Locatelli F, Fruci D
    Cancer Res, 2015;75(5):824-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Recombinant Proteins
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Recombinant Human IgG1 Fc, CF

BA 110-HG 66
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