Recombinant Human LAIR2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10166-LR-050
Recombinant Human LAIR2 Fc Chimera Protein, CF Binding Activity
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Recombinant Human LAIR2 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Bovine Collagen I is coated at 10 µg/mL, 100 μL/well, Recombinant Human LAIR2 Fc Chimera binds with an ED50 of 0.04-0.32 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human LAIR2 protein
Human LAIR2
(Gln22-Pro152)
Accession # Q6ISS4
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Glu23 inferred from enzymatic pyroglutamate treatment revealing Gln22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
41 kDa
SDS-PAGE
50-56 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10166-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Bovine Collagen I is coated at 10 µg/mL, 100 μL/well,Recombinant Human LAIR2 Fc Chimera (Catalog # 10166-LR) binds with an ED50of 0.04-0.32 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human LAIR2 Fc Chimera (Catalog # 10166-LR) was resolvedwith SDS-PAGE under reducing (R) and non-reducing (NR) conditions andvisualized by Coomassie® Blue staining, showing bands at 50-56 kDa and 100-120kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAIR2

LAIR2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR1, its transmembrane counterpart, LAIR2 shares 83% aa identity across the signal sequence and extracellular domains (1-3). Although one is secreted and the other is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR2 may compete with LAIR1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR-2 is truncated at the C-terminus, but retains the entire Ig domain (1-3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported. LAIR2 is a soluble collagen-receptor, and it can be detected in the synovial fluid of rheumatoid arthritis patients, urine of pregnant women, and as well as primary cells (5, 6). In vitro studies have demonstrated LAIR2 can compete with LAIR1 for the same collagen binding site and suggesting LAIR2 may play an important role in immune cell activation (5, 6). LAIR2 can interact with complement component 1q (C1q) and mannose-binding lectin (MBL) and act as a complement inhibitor for the treatment and prevention of antibody-mediated allograft rejection and antibody-mediated clinical conditions (7).

References
  1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
  2. Meyaard, L. et al. (1999) J. Immunol. 162:5800.
  3. Meyaard, L. et al. (1997) Immunity 7:283.
  4. Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.
  5. Lebbink, R.J. et al. (2008) J. Immunol. 180:1662.
  6. Olde Nordkamp, M.J. et al. (2011) Arthritis Rheum. 63:3749.
  7. Olde Nordkamp, M.J. et al. (2014) J. Innate Immun. 6:284.
Long Name
Leukocyte-associated Ig-like Receptor 2
Entrez Gene IDs
3904 (Human)
Alternate Names
CD306 antigen; CD306; CD306leukocyte-associated Ig-like receptor-2; LAIR2; LAIR-2; leukocyte-associated Ig-like receptor 2; leukocyte-associated immunoglobulin-like receptor 2; MGC71634

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