Recombinant Human Latent BMP-9 Protein

Carrier Free

Catalog # Availability Size / Price Qty
9624-BP-025/CF

With Carrier

Catalog # Availability Size / Price Qty
9624-BP-025
Recombinant Human Latent BMP-9 Protein Bioactivity
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Product Details
FAQs
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Recombinant Human Latent BMP-9 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 1-6 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-9 protein
Lys23-Arg319 (pro) & Ser320-Arg429 (mature)
Accession #
N-terminal Sequence
Analysis
Lys23 (pro) & Ser320 (mature)
Structure / Form
Non covalent complex between the prodomain and mature domain.
Predicted Molecular Mass
33 kDa (pro) & 12 kDa (mature)
SDS-PAGE
39-44 kDa (pro) & 11 kDa (mature)

Product Datasheets

9624-BP (with carrier)

9624-BP/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9624-BP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9624-BP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human Latent BMP-9 Protein Bioactivity View Larger

Recombinant Human Latent BMP-9 (Catalog # 9624-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 1-6 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-9

Human BMP-9, also known as growth and differentiation factor 2 (GDF2), is a member of the BMP subgroup of the TGF beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by nonparenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK1 (4, 6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8, 10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

References
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136.
  4. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
  5. Song, J.J. et al. (1995) Endocrinology 136:4293.
  6. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
  7. Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
  8. LopezCoviella,I. et al. (2000) Science 289:313.
  9. LopezCoviella,I. et al. (2005) Proc. Natl. Acad. Sci. 102:6984.
  10. LopezCoviella,I. et al. (2002) J. Physiol. Paris 96:53.
  11. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
  12. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
  13. Ploemacher, R.E. et al. (1999) Leukemia 13:428.
Long Name
Bone Morphogenetic Protein 9
Entrez Gene IDs
2658 (Human); 12165 (Mouse)
Alternate Names
BMP9; BMP-9; BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2

FAQs

  1. What is the half-life of a recombinant or natural protein in culture at 37 °C?

    • R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

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