Recombinant Human Latrophilin 3 Protein Fc Chimera, CF

R&D Systems | Catalog # 8915-LN

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Latrophilin 3 Protein Fc Chimera (8915-LN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_056051.2

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Latrophilin 3/LPHN3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Latrophilin 3/LPHN3 protein
Human Latrophilin 3
(Phe20-Leu854)
Accession # NP_056051		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe20

Predicted Molecular Mass

120 kDa

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Latrophilin 3/LPHN3 Fc Chimera is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human FLRT3 (Catalog # 2795-FL) that produces 50% of the optimal binding response is approximately 0.07-0.35 μg/mL

Formulation, Preparation, and Storage

8915-LN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Latrophilin 3/LPHN3

Latrophilin-3 (LPHN3) is a 7-transmembrane protein in the family of adhesion GPCRs with large N-terminal extracellular regions (1, 2). The 847 amino acid (aa)
N-terminal region of human Latrophilin-3 contains a SUEL-like lectin domain, an Olfactomedin-like domain, an O-glycosylated mucin-like region, a hormone receptor domain, and a GPCR proteolysis site (GPS). Within this approximately 120 kDa region, human Latrophilin-3 shares 96% aa sequence identity with mouse and rat Latrophilin-3. Alternative splicing of human Latrophilin-3 generates additional isoforms with a 13 aa insertion or premature truncation in the N-terminal ECD (3). Latrophilin-3 is expressed in the brain cortex (3). It binds to FLRT1, 2, and 3 and Tenurin 1 (4, 5). Its binding in trans to post-synaptic FLRT3 at glutamatergic synapses supports the maintenance of excitatory synapses as well as the amplitude of glutamatergic transmission (4, 5). Polymorphisms of Latrophilin-3 are associated with susceptibility to attention deficit/hyperactivity disorder (ADHD) (3, 6). Latrophilin-3 is also expressed in fetal adrenal glands and is down-regulated in the adult (7).

References

  1. Promel, S. et al. (2012) Cell Rep. 2:321.
  2. Matsushita, H. et al. (1999) FEBS Lett. 443:348.
  3. Arcos-Burgos, M. et al. (2010) Mol. Pschiatry 15:1053.
  4. O’Sullivan, M.L. et al. (2012) Neuron 73:903.
  5. O’Sullivan, M.L. et al. (2014) Neural Dev. 9:7.
  6. Franke, B. et al. (2012) Mol. Psychiatry 17:960.
  7. Xing, Y. et al. (2009) Mol. Cell. Endocrinol. 300:43.

Alternate Names

CIRL3, LEC3, Lectomedin-3, LPHN3

Entrez Gene IDs

23284 (Human); 319387 (Mouse); 170641 (Rat)

Gene Symbol

ADGRL3

UniProt

NP_056051.2

Additional Latrophilin 3/LPHN3 Products

Product Documents for Recombinant Human Latrophilin 3 Protein Fc Chimera, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Latrophilin 3 Protein Fc Chimera, CF

For research use only

Citations for Recombinant Human Latrophilin 3 Protein Fc Chimera, CF

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