Recombinant Human LBP Protein, CF

Newer Version Available: 6445-LP/CF
A New rh LBP is Now Available! The new protein has a His-tag!
Formulations:
Catalog # Availability Size / Price Qty
870-LP-025/CF
Product Details
Citations (12)
FAQs
Reviews

Recombinant Human LBP Protein, CF Summary

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP‑1 human acute monocytic leukemia cells. The ED50 for this effect is 0.25‑2.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human LBP protein
Ala26-Val481
Accession #
N-terminal Sequence
Analysis
Ala26
Predicted Molecular Mass
51 kDa
SDS-PAGE
55-60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

870-LP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

870-LP

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LBP

Lipopolysaccharide-binding protein (LBP), a 58 kDa glycoprotein synthesized in hepatocytes, belongs to a family of lipid-binding proteins which includes bactericidal/permeability increasing protein (BPI), phospholipid ester transfer protein (PLTP), and cholesterol ester transfer protein (CETP). LBP binds to the lipid A portion of lipopolysaccharide (LPS) to facilitate the process of LPS monomerization, catalyze the binding of LPS monomers to CD14, and promote LPS-induced immune response. LBP is present at low concentrations in normal human serum and may increase up to 30-fold during the acute phase response. Studies indicate that LBP also acts catalytically in the transfer of LPS to HDL, thus accelerating LPS detoxification. In addition, LBP and soluble CD14 can also function in phospholipid transport.

References
  1. Hailman, E. et al. (1994) J. Exp. Med. 179:269.
  2. Jack, R.S. et al. (1997) Nature 389:742.
  3. Schumann, R.R. et al. (2000) Chem. Immunol. 74:42.
Long Name
Lipopolysaccharide-binding Protein
Entrez Gene IDs
3929 (Human); 16803 (Mouse)
Alternate Names
LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; LPS-binding protein; MGC22233

Citations for Recombinant Human LBP Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming
    Authors: AJ Hoogendijk, F Pourfarzad, CEM Aarts, ATJ Tool, IH Hiemstra, L Grassi, M Frontini, AB Meijer, M van den Bi, TW Kuijpers
    Cell Rep, 2019;29(8):2505-2519.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Differential regulation of the transcriptomic and secretomic landscape of sensor and effector functions of human airway epithelial cells
    Authors: R Lehmann, MM Müller, TE Klassert, D Driesch, M Stock, A Heinrich, T Conrad, C Moore, UK Schier, R Guthke, H Slevogt
    Mucosal Immunol, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Tie2 protects the vasculature against thrombus formation in systemic inflammation
    Authors: SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4
    Authors: B S Lashkari, DO Anumba
    PLoS ONE, 2017;12(3):e0173646.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. 3LPS-binding protein and its interactions with P. gingivalis LPS modulate pro-inflammatory response and Toll-like receptor signaling in human oral keratinocytes
    Authors: PH Ding, RP Darveau, CY Wang, L Jin
    PLoS ONE, 2017;12(4):e0173223.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Granzymes A and K differentially potentiate LPS-induced cytokine response
    Authors: Annette C Wensink
    Cell Death Discov, 2016;2(0):16084.
    Applications: Bioassay
  7. Human CalDAG-GEFI gene (RASGRP2) mutation affects platelet function and causes severe bleeding.
    Authors: Canault M, Ghalloussi D, Grosdidier C, Guinier M, Perret C, Chelghoum N, Germain M, Raslova H, Peiretti F, Morange P, Saut N, Pillois X, Nurden A, Cambien F, Pierres A, van den Berg T, Kuijpers T, Alessi M, Tregouet D
    J Exp Med, 2014;211(7):1349-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Directed evolution of an LBP/CD14 inhibitory peptide and its anti-endotoxin activity.
    Authors: Fang L, Xu Z, Wang G, Ji F, Mei C, Liu J, Wu G
    PLoS ONE, 2014;9(7):e101406.
    Species: Human
    Sample Types: Virus
    Applications: Enzyme Assay
  9. Proteasome proteolysis supports stimulated platelet function and thrombosis.
    Authors: Gupta N, Li W, Willard B, Silverstein R, McIntyre T
    Arterioscler Thromb Vasc Biol, 2014;34(1):160-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Ovocalyxin-36 is a pattern recognition protein in chicken eggshell membranes.
    Authors: Cordeiro, Cristian, Esmaili, Hamed, Ansah, George, Hincke, Maxwell
    PLoS ONE, 2013;8(12):e84112.
    Species: Chicken
    Sample Types: Tissue Homogenates
    Applications: Bioassay
  11. Staphylococcus aureus leukotoxin GH promotes formation of neutrophil extracellular traps.
    Authors: Malachowa N, Kobayashi S, Freedman B, Dorward D, DeLeo F
    J Immunol, 2013;191(12):6022-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding.
    Authors: Sakaguchi M, Murata H, Yamamoto K, Ono T, Sakaguchi Y, Motoyama A, Hibino T, Kataoka K, Huh NH
    PLoS ONE, 2011;6(8):e23132.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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