Recombinant Human LIGHT/TNFSF14 Protein, CF

(10 citations)   
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cytotoxicity assay using HT‑29 human colon adenocarcinoma cells Yu, K.Y. et al. (1999) J. Biol. Chem. 274:13733; Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548; Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142. Recombinant Human LIGHT/TNFSF14 at 10 ng/mL causes a 2-4.5 fold inhibition of HT29 proliferation, in the presence of 10 U/mL (1 ng/mL) Recombinant Human IFN-gamma (Catalog # 285-IF). Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-4 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived
    Met 10-His tag GGGSGGGSGGGSIEGR Human Light
    (Asp74-Val240)
    Accession # O43557
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    20.9 kDa
  • SDS-PAGE
    25 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
664-LI/CF
 
664-LI
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images

Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-4 ng/mL.

1 µg/lane of Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 25 kDa.

Background: LIGHT/TNFSF14

Human LIGHT is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma. The full roles of LIGHT remain to be elucidated.

  • References:
    1. Mauri, D.N. et al. (1998) Immunity 8:21.
    2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
    3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
    4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.
  • Long Name:
    TNF Ligand Superfamily Member 14
  • Entrez Gene IDs:
    8740 (Human); 50930 (Mouse)
  • Alternate Names:
    CD258 antigen; CD258; delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator ligand; HVEM-L; HVEMLherpesvirus entry mediator-ligand; ligand for herpesvirus entry mediator; LIGHT; LIGHTherpesvirus entry mediator A; LTg; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. LIGHT (TNFSF14) Increases the Survival and Proliferation of Human Bone Marrow-Derived Mesenchymal Stem Cells
    PLoS ONE, 2016;11(11):e0166589.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. A TNFRSF14-Fc?RI-mast cell pathway contributes to development of multiple features of asthma pathology in mice
    Nat Commun, 2016;7(0):13696.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
    Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
    J Immunol, 2015;195(5):2429-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis.
    Authors: Herro R, Antunes R, Aguilera A, Tamada K, Croft M
    J Invest Dermatol, 2015;135(8):2109-18.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Lymphotoxin beta receptor signaling induces IL-8 production in human bronchial epithelial cells.
    Authors: Mikami Y, Matsuzaki H, Horie M, Noguchi S, Jo T, Narumoto O, Kohyama T, Takizawa H, Nagase T, Yamauchi Y
    PLoS ONE, 2014;9(12):e114791.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-kappaB pathway.
    Authors: Jin HR, Jin X, Lee JJ
    Biochem. Biophys. Res. Commun., 2010;400(4):581-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Inflammatory interaction between LIGHT and proteinase-activated receptor-2 in endothelial cells: potential role in atherogenesis.
    Authors: Sandberg WJ, Halvorsen B, Yndestad A, Smith C, Otterdal K, Brosstad FR, Froland SS, Olofsson PS, Damas JK, Gullestad L, Hansson GK, Oie E, Aukrust P
    Circ. Res., 2009;104(1):60-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes.
    Authors: Kang YM, Kim SY, Kang JH
    Arthritis Rheum., 2007;56(4):1106-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis.
    Authors: Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A
    Arthritis Rheum., 2006;54(5):1451-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
    Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
    Blood, 2006;108(3):928-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Primary Antibodies
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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ICFlow IC050G  
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ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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