Recombinant Human LILRA6/CD85b/ILT8 Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human LILRA6/CD85b/ILT8 is coated onto a microplate at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 = 0.1-0.6 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived human LILRA6/CD85b protein
    Gly24-Asn447, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Gly24
  • Predicted Molecular Mass
    47 kDa
  • SDS-PAGE
    62-69 kDa, reducing conditions
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9088-T4
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 500 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
When Recombinant Human LILRA6/CD85b/ILT8 is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7 (Catalog #
914-AN) binds with a typical ED50 of
0.1-0.6 µg/mL.
Background: LILRA6/CD85b
LILRA6, also known as ILT8 and CD85b, is a transmembrane molecule that belongs to the LILR family of immune regulatory proteins (1, 2). Among family members, LILRA6 is most closely related to LILRB3/CD85a/ILT5; these proteins share 94% amino acid (aa) sequence identity in their extracellular domains (ECD). Mature human LILRA6 consists of a 424 aa ECD with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 13 aa cytoplasmic domain (3). It contains a positively charged arginine residue in the transmembrane segment which may mediate association with the signaling protein Fc epsilon  RI gamma. Alternative splicing generates a short isoform that is truncated near the middle of the ECD. LILRA6 is highly polymorphic, and its gene is found in variably copy numbers (4, 5). LILRA6 is primarily expressed on monocytes (4). R&D Systems in-house testing indicates that LILRA6 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (6).
  • References:
    1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
    2. Hirayasu, K. and H. Arase (2015) J. Hum. Genet. 60:703.
    3. Wende, H. et al. (2000) Immunogenetics 51:703.
    4. Bashirova, A.A. et al. (2014) Immunogenetics 66:1.
    5. Lopez-Alvarez, M.R. et al. (2014) Immunogenetics 66:73.
    6. Zheng, J. et al. (2012) Nature 485:656.
  • Long Name:
    Leukocyte Immunoglobulin-like Receptor A6
  • Entrez Gene IDs:
    79168 (Human); 18726 (Mouse)
  • Alternate Names:
    7M1; CD85b; ILT8; LILRA6; Pira3
Related Research Areas

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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 28  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 3  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag Antibody

WB , Flow , AP MAB050R 2  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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