Recombinant Human LILRA6/CD85b/ILT8 Protein, CF

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Recombinant Human LILRA6/CD85b/ILT8 Protein Bioactivity
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Recombinant Human LILRA6/CD85b/ILT8 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human LILRA6/CD85b/ILT8 is coated onto a microplate at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 = 0.1-0.6 μg/mL.
Human embryonic kidney cell, HEK293-derived human LILRA6/CD85b protein
Gly24-Asn447, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
47 kDa
62-69 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human LILRA6/CD85b/ILT8 Protein Bioactivity View Larger

When Recombinant Human LILRA6/CD85b/ILT8 is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7 (Catalog # 914-AN) binds with a typical ED50 of 0.1-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: LILRA6/CD85b

LILRA6, also known as ILT8 and CD85b, is a transmembrane molecule that belongs to the LILR family of immune regulatory proteins (1, 2). Among family members, LILRA6 is most closely related to LILRB3/CD85a/ILT5; these proteins share 94% amino acid (aa) sequence identity in their extracellular domains (ECD). Mature human LILRA6 consists of a 424 aa ECD with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 13 aa cytoplasmic domain (3). It contains a positively charged arginine residue in the transmembrane segment which may mediate association with the signaling protein Fc epsilon  RI gamma. Alternative splicing generates a short isoform that is truncated near the middle of the ECD. LILRA6 is highly polymorphic, and its gene is found in variably copy numbers (4, 5). LILRA6 is primarily expressed on monocytes (4). R&D Systems in-house testing indicates that LILRA6 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (6).

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Hirayasu, K. and H. Arase (2015) J. Hum. Genet. 60:703.
  3. Wende, H. et al. (2000) Immunogenetics 51:703.
  4. Bashirova, A.A. et al. (2014) Immunogenetics 66:1.
  5. Lopez-Alvarez, M.R. et al. (2014) Immunogenetics 66:73.
  6. Zheng, J. et al. (2012) Nature 485:656.
Long Name
Leukocyte Immunoglobulin-like Receptor A6
Entrez Gene IDs
79168 (Human); 18726 (Mouse)
Alternate Names
7M1; CD85b; CD85b;ILT5;ILT8;ILT-8;LILRB3;LILRB6; ILT8; LILRA6; Pira3


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By Anonymous on 05/06/2020
Application: Binding assay/Protein-protein interaction
Reason for Rating: pure material/ no binding observed (non-target IgG)

Performed binding specificity experiments for the lead molecule (xLILRA6) on its ligand family panels (RA1, RA2, RA5, RA6, RB1, RB2, RB3, RB4, RB5)