>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human LILRA6/CD85b/ILT8 is coated onto a microplate at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7
binds with a typical ED50 = 0.1-0.6 μg/mL.
Human embryonic kidney cell, HEK293-derived Gly24-Asn447, with a C-terminal 6-His tag
When Recombinant Human LILRA6/CD85b/ILT8 is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7 (Catalog # 914-AN) binds with a typical ED50 of 0.1-0.6 µg/mL.
LILRA6, also known as ILT8 and CD85b, is a transmembrane molecule that belongs to the LILR family of immune regulatory proteins (1, 2). Among family members, LILRA6 is most closely related to LILRB3/CD85a/ILT5; these proteins share 94% amino acid (aa) sequence identity in their extracellular domains (ECD). Mature human LILRA6 consists of a 424 aa ECD with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 13 aa cytoplasmic domain (3). It contains a positively charged arginine residue in the transmembrane segment which may mediate association with the signaling protein Fc epsilon RI gamma. Alternative splicing generates a short isoform that is truncated near the middle of the ECD. LILRA6 is highly polymorphic, and its gene is found in variably copy numbers (4, 5). LILRA6 is primarily expressed on monocytes (4). R&D Systems in-house testing indicates that LILRA6 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (6).
Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
Hirayasu, K. and H. Arase (2015) J. Hum. Genet. 60:703.
Wende, H. et al. (2000) Immunogenetics 51:703.
Bashirova, A.A. et al. (2014) Immunogenetics 66:1.
Lopez-Alvarez, M.R. et al. (2014) Immunogenetics 66:73.
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