Recombinant Human Angiopoietin-like 7 Protein

R&D Systems | Catalog # 914-AN

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human Angiopoietin-like 7 Protein (914-AN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

O43827

Applications

Bioactivity
View all Angiopoietin-like Protein 7/ANGPTL7 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 7/ANGPTL7 protein
Met1-Pro346, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln27 predicted

Predicted Molecular Mass

38.6 kDa

SDS-PAGE

49-55 kDa, reducing conditions

Activity

Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL).
The ED50 for this effect is 50-200 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).

Reviewed Applications

Read 1 review rated 5 using 914-AN in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 914-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 914-AN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 7/ANGPTL7

Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (1 - 3). ANGPTL7 shares 89% aa sequence identity with mouse and rat ANGPTL7. It is secreted as a 45 ‑ 50 kDa monomer that forms disulfide-linked homotrimers and tetramers via the coiled coil domain (4, 5). ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor (3, 5). Its production is up‑regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha (5 - 7). Overexpression of ANGPTL7 in trabecular meshwork cells inhibits the production of collagen and proteoglycans (8). When overexpressed in tumor cells it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis (4). Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells (9).

References

  1. Oike, Y. et al. (2009) Circ. J. 73:2192.
  2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
  3. Peek, R. et al. (1998) Invest. Opthalmol. Visual Sci. 39:1782.
  4. Peek, R. et al. (2002) J. Biol. Chem. 277:686.
  5. Kuchtey, J. et al. (2008) Invest. Opthalmol. Visual Sci. 49:3438.
  6. Nehme, A. et al. (2009) BMC Med. Genomics 2:58.
  7. Stevens, A.L. et al. (2008) Arthritis Rheum. 58:489.
  8. Comes, N. et al. (2010) Genes Cells 16:243.
  9. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Long Name

Angiopoietin-like Protein 7

Alternate Names

ANGPTL7, AngX, CDT6

Entrez Gene IDs

10218 (Human); 654812 (Mouse); 102127960 (Cynomolgus Monkey); 714204 (Rhesus Macaque)

Gene Symbol

ANGPTL7

UniProt

O43827

Additional Angiopoietin-like Protein 7/ANGPTL7 Products

Product Documents for Recombinant Human Angiopoietin-like 7 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 914-AN (with carrier)
Download SDS 914-AN/CF (carrier free)

Product Specific Notices for Recombinant Human Angiopoietin-like 7 Protein

For research use only

Citations for Recombinant Human Angiopoietin-like 7 Protein

Customer Reviews for Recombinant Human Angiopoietin-like 7 Protein (1)

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    Recombinant Human Angiopoietin-like 7 Protein 914-AN

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FAQs for Recombinant Human Angiopoietin-like 7 Protein

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  • Q:

    This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    A: The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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