Recombinant Human LILRB5/CD85c/LIR-8 Protein, CF

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Recombinant Human LILRB5/CD85c/LIR-8 Protein Binding Activity
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Recombinant Human LILRB5/CD85c/LIR-8 Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human LILRB5/CD85c/LIR-8 is coated at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an apparent Kd <4 nM.
Mouse myeloma cell line, NS0-derived human LILRB5/CD85c/LIR-8 protein
Arg18-His456, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
49 kDa
64-77 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human LILRB5/CD85c/LIR-8 Protein Binding Activity View Larger

When Recombinant Human LILRB5/CD85c/LIR-8 is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an apparent Kd<4 nM.

Reconstitution Calculator

Reconstitution Calculator

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Background: LILRB5/CD85c/LIR-8

LIR8, also known as CD85c and LILRB5, belongs to the Leukocyte immunoglobulin-like receptors (LILR) family of transmembrane glycoproteins involved in regulating immune responses (1,2). There are at least thirteen LILR family members and are divided into activating (LILRA) or inhibiting (LILRB) molecules (1,2). Mature human LIR8 consists of an extracellular domain (ECD) with four Ig-like domains, a transmembrane segment, and a cytoplasmic domain with two immunoreceptor tyrosine-based inhibitory motifs (ITIM). Alternative splicing of human LIR8 generates at least 2 isoforms, one lacking the second Ig-like domain. The LILR family appears to be primate-specific receptors in terms of sequence homology. LIR8 is expressed on NK cells and in the tryptic granules of mast cells and negatively regulates immune cell activation (3, 4). It is present on the mast cell surface following cell activation and degranulation (4). Activated mast cells may also release soluble forms of LIR8 (3). LIR8 has also been shown to be expressed on T cells and induce CD8+ T cell proliferation (5).  Consistent with the demonstrated binding of LILRB2 to Angiopoietin-like 2 and 5 (6), R&D Systems in-house testing indicates that LIR8 binds to Angiopoietin-like 7. Recently, a common missense variant of LIR8 was found to be associated with statin intolerance and myalgia (7).

  1. Brown, D. et al. (2004) Tissue antigens. 64:215.
  2. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  3. Borges, L. et al. (1997) J. Immunol. 159:5192.
  4. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  5. Hogan, H. E. et al. (2016) Sci Rep. 6:21780.
  6. Zheng, J. et al. (2012) Nature 485:656.
  7. Siddigui, M. et al. (2017) Eur. Heart J. 38:3569.
Long Name
Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM And ITIM Domains), Member 5
Entrez Gene IDs
10990 (Human); 102129427 (Cynomolgus Monkey)
Alternate Names
CD85 antigen-like family member C; CD85c antigen; CD85c; Leukocyte immunoglobulin-like receptor 8; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB5; LIR8; LIR-8; LIR8CD85C; LIR-8leukocyte immunoglobulin-like receptor subfamily B member 5; member 5


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By Anonymous on 05/06/2020
Application: Binding assay/Protein-protein interaction
Reason for Rating: good material/ no binding observed on our lead molecule

Performed binding specificity experiments for the lead molecule (xLILRA6) on its ligand family panels (RA1, RA2, RA5, RA6, RB1, RB2, RB3, RB4, RB5)