Recombinant Human LILRB5/CD85c/LIR-8 Protein, CF

R&D Systems | Catalog # 8487-T4

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human LILRB5/CD85c/LIR-8 Protein (8487-T4)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

O75023

Applications

Bioactivity
View all LILRB5/CD85c/LIR-8 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human LILRB5/CD85c/LIR-8 protein
Arg18-His456, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg18

Predicted Molecular Mass

49 kDa

SDS-PAGE

64-77 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LILRB5/CD85c/LIR-8 is coated at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an apparent Kd <4 nM.

Reviewed Applications

Read 1 review rated 5 using 8487-T4 in the following applications:

Scientific Data Images for Recombinant Human LILRB5/CD85c/LIR-8 Protein, CF

Recombinant Human LILRB5/CD85c/LIR-8 Protein Binding Activity

Recombinant Human LILRB5/CD85c/LIR-8 Protein Binding Activity

When Recombinant Human LILRB5/CD85c/LIR-8 is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an apparent Kd<4 nM.

Formulation, Preparation, and Storage

8487-T4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRB5/CD85c/LIR-8

LIR8, also known as CD85c and LILRB5, belongs to the Leukocyte immunoglobulin-like receptors (LILR) family of transmembrane glycoproteins involved in regulating immune responses (1,2). There are at least thirteen LILR family members and are divided into activating (LILRA) or inhibiting (LILRB) molecules (1,2). Mature human LIR8 consists of an extracellular domain (ECD) with four Ig-like domains, a transmembrane segment, and a cytoplasmic domain with two immunoreceptor tyrosine-based inhibitory motifs (ITIM). Alternative splicing of human LIR8 generates at least 2 isoforms, one lacking the second Ig-like domain. The LILR family appears to be primate-specific receptors in terms of sequence homology. LIR8 is expressed on NK cells and in the tryptic granules of mast cells and negatively regulates immune cell activation (3, 4). It is present on the mast cell surface following cell activation and degranulation (4). Activated mast cells may also release soluble forms of LIR8 (3). LIR8 has also been shown to be expressed on T cells and induce CD8+ T cell proliferation (5).  Consistent with the demonstrated binding of LILRB2 to Angiopoietin-like 2 and 5 (6), R&D Systems in-house testing indicates that LIR8 binds to Angiopoietin-like 7. Recently, a common missense variant of LIR8 was found to be associated with statin intolerance and myalgia (7).

References

  1. Brown, D. et al. (2004) Tissue antigens. 64:215.
  2. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  3. Borges, L. et al. (1997) J. Immunol. 159:5192.
  4. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  5. Hogan, H. E. et al. (2016) Sci Rep. 6:21780.
  6. Zheng, J. et al. (2012) Nature 485:656.
  7. Siddigui, M. et al. (2017) Eur. Heart J. 38:3569.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM And ITIM Domains), Member 5

Alternate Names

CD85c, LIR-8, LIR8

Entrez Gene IDs

10990 (Human); 102129427 (Cynomolgus Monkey)

Gene Symbol

LILRB5

UniProt

O75023

Additional LILRB5/CD85c/LIR-8 Products

Product Documents for Recombinant Human LILRB5/CD85c/LIR-8 Protein, CF

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Product Specific Notices for Recombinant Human LILRB5/CD85c/LIR-8 Protein, CF

For research use only

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  • Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/06/2020
    Performed binding specificity experiments for the lead molecule (xLILRA6) on its ligand family panels (RA1, RA2, RA5, RA6, RB1, RB2, RB3, RB4, RB5)
    Recombinant Human LILRB5/CD85c/LIR-8 Protein, CF 8487-T4

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