Recombinant Human LRG1 Protein, CF

Catalog # Availability Size / Price Qty
7890-LR-025
Product Details
Citations (1)
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Recombinant Human LRG1 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When human Cytochrome c is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human LRG1 that produces 50% of the optimal binding response is 3-15 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human LRG1 protein
Val36-Gln347, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Val36
Predicted Molecular Mass
35.2 kDa
SDS-PAGE
40-60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7890-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: LRG1

LRG1 (leucine-rich alpha -2-glycoprotein 1) is an approximately 50 kDa secreted glycoprotein present in the plasma and other body fluids (1‑4). The human LRG1 cDNA encodes 347 amino acids (aa) that include a 35 aa signal sequence, eight leucine‑rich repeats (LRR), and an LRRCT (LRR C‑terminal) domain (1). Mature human LRG1 shares 66%, 63%, 75% and 72% aa sequence identity with mouse, rat, canine and porcine LRG1, respectively. LRG1 is up‑regulated during differentiation of neutrophils and present with myeloperoxidase in primary neutrophilic granules (1, 2). It can be produced by hepatocytes as an acute phase protein (5). LRG1 can interact directly with Cytochrome c, and is thought to act as a survival factor for lymphocytes in the presence of extracellular Cytochrome c (3, 6). Serum or plasma LRG1 is reported to be elevated in toxic shock syndrome, cardiac damage (produced by cardiomyocytes and correlating with BNP), autoimmune syndromes such as rheumatoid arthritis and Crohn’s disease, and some cancers such as those in the ovary and biliary tract (4, 7‑10). It is elevated in coronary sinus fluid during ventricular dysfunction and heart failure, and in peritoneal fluid of patients with uterine lyomyomas (8, 11).

References
  1. O’Donnell, L.C. et al. (2002) J. Leukoc. Biol. 72:478.
  2. Ai, J. et al. (2008) J. Leukoc. Biol. 83:1277.
  3. Cummings, C. et al. (2006) Apoptosis 11:1121.
  4. Weivoda, S. et al. (2008) J. Immunol. Methods 336:22.
  5. Shirai, R. et al. (2009) Biochem. Biophys. Res. Commun. 382:776.
  6. Codina, R. et al. (2010) Apoptosis 15:139.
  7. Serada, S. et al. (2010) Ann. Rheum. Dis. 69:770.
  8. Watson, C.J. et al. (2011) Circ. Heart Fail. 4:188.
  9. Andersen, J.D. et al. (2010) J. Ovarian Res. 3:21.
  10. Sandanayake, N.S. et al. (2011) Br. J. Cancer 105:1370.
  11. Ferrero, S. et al. (2009) Arch. Gynecol. Obstet. 279:365.
Long Name
Leucine-rich alpha-2-glycoprotein 1
Entrez Gene IDs
116844 (Human); 76905 (Mouse); 367455 (Rat)
Alternate Names
1300008B03Rik; 2310031E04Rik; HMFT1766; leucine-rich alpha-2-glycoprotein 1; leucine-rich alpha-2-glycoprotein; LRG1; LRGleucine rich alpha 2 glycoprotein

Citation for Recombinant Human LRG1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. LRG1 modulates epithelial-mesenchymal transition and angiogenesis in colorectal cancer via HIF-1alpha activation.
    Authors: Zhang J, Zhu L, Fang J, Ge Z, Li X
    J Exp Clin Cancer Res, 2016;35(1):29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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