Recombinant Human LRG1 Protein, CF
Recombinant Human LRG1 Protein, CF Summary
Val36-Gln347, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
LRG1 (leucine-rich alpha -2-glycoprotein 1) is an approximately 50 kDa secreted glycoprotein present in the plasma and other body fluids (1‑4). The human LRG1 cDNA encodes 347 amino acids (aa) that include a 35 aa signal sequence, eight leucine‑rich repeats (LRR), and an LRRCT (LRR C‑terminal) domain (1). Mature human LRG1 shares 66%, 63%, 75% and 72% aa sequence identity with mouse, rat, canine and porcine LRG1, respectively. LRG1 is up‑regulated during differentiation of neutrophils and present with myeloperoxidase in primary neutrophilic granules (1, 2). It can be produced by hepatocytes as an acute phase protein (5). LRG1 can interact directly with Cytochrome c, and is thought to act as a survival factor for lymphocytes in the presence of extracellular Cytochrome c (3, 6). Serum or plasma LRG1 is reported to be elevated in toxic shock syndrome, cardiac damage (produced by cardiomyocytes and correlating with BNP), autoimmune syndromes such as rheumatoid arthritis and Crohn’s disease, and some cancers such as those in the ovary and biliary tract (4, 7‑10). It is elevated in coronary sinus fluid during ventricular dysfunction and heart failure, and in peritoneal fluid of patients with uterine lyomyomas (8, 11).
- O’Donnell, L.C. et al. (2002) J. Leukoc. Biol. 72:478.
- Ai, J. et al. (2008) J. Leukoc. Biol. 83:1277.
- Cummings, C. et al. (2006) Apoptosis 11:1121.
- Weivoda, S. et al. (2008) J. Immunol. Methods 336:22.
- Shirai, R. et al. (2009) Biochem. Biophys. Res. Commun. 382:776.
- Codina, R. et al. (2010) Apoptosis 15:139.
- Serada, S. et al. (2010) Ann. Rheum. Dis. 69:770.
- Watson, C.J. et al. (2011) Circ. Heart Fail. 4:188.
- Andersen, J.D. et al. (2010) J. Ovarian Res. 3:21.
- Sandanayake, N.S. et al. (2011) Br. J. Cancer 105:1370.
- Ferrero, S. et al. (2009) Arch. Gynecol. Obstet. 279:365.
Citations for Recombinant Human LRG1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Biomarker Panel for the Diagnosis of Pancreatic Ductal Adenocarcinoma
Authors: H Kim, KN Kang, YS Shin, Y Byun, Y Han, W Kwon, CW Kim, JY Jang
Cancers (Basel), 2020;12(6):.
Sample Types: Plasma
Applications: ELISA (standard)
LRG1 modulates epithelial-mesenchymal transition and angiogenesis in colorectal cancer via HIF-1alpha activation.
Authors: Zhang J, Zhu L, Fang J, Ge Z, Li X
J Exp Clin Cancer Res, 2016;35(1):29.
Sample Types: Whole Cells
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